Patents by Inventor John W. Randles

John W. Randles has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Use of a virus DNA as promoter
    Patent number: 6303345
    Abstract: Described is the characterization and the use of strong viral promoters for expressing genes, in bacteria and fungi, in particular yeasts. The invention is based on the surprising finding that CFDV DNA (coconut foliar decay virus DNA) and CFDV DNA fragments contain a region which is active as promoter even in bacteria and fungi, in particular yeasts, although they are derived from a virus which infects monocotyledonous plants. The activity of the promoters described in E. coli is distinctly higher than that of the CaMV 35S promoter, which is also active in bacteria.
    Type: Grant
    Filed: May 17, 2000
    Date of Patent: October 16, 2001
    Assignee: Max-Planck-Gesellschaft zur Forderung der Wissenschaften E.V.
    Inventors: Wolfgang Rohde, Dieter Becker, John W. Randles, Alain Hehn, Francesco Salamini
  • Ribozyme-mediated synthesis of circular RNA
    Patent number: 6210931
    Abstract: Circular RNAs may be synthesized by inserting DNA fragments into a plasmid containing sequences having the capability of spontaneous cleavage and self-circularization. Insertion of the DNA fragments allows RNAs of predetermined size to be constructed. In addition, a two-dimensional polyacrylamide gel electrophoresis system having a second dimension more highly cross-linked than the first dimension permits the separation and analysis as well as the precise sizing of both linear and circular RNAs produced by the synthetic method.
    Type: Grant
    Filed: November 30, 1998
    Date of Patent: April 3, 2001
    Assignee: The United States of America as represented by the Secretary of Agriculture
    Inventors: Paul A. Feldstein, Robert A. Owens, Laurene Levy, John W. Randles