Patents by Inventor John W. Rumsey

John W. Rumsey has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Method for culturing skeletal muscle for tissue engineering
    Patent number: 10160953
    Abstract: The invention provides a nutrient medium composition and associated methods for lengthening the useful life of a culture of muscle cells. Disclosed is a method of culturing mammalian muscle cells, including preparing one or more carriers coated with a covalently bonded monolayer of trimethoxy-silylpropyl-diethylenetriamine (DETA); verifying DETA monolayer formation by one or more associated optical parameters; suspending isolated fetal rat skeletal muscle cells in serum-free medium according to medium composition 1; plating the suspended cells onto the prepared carriers at a predetermined density; leaving the carriers undisturbed for cells to adhere to the DETA monolayer; covering the carriers with a mixture of medium 1 and medium 2; and incubating.
    Type: Grant
    Filed: October 19, 2015
    Date of Patent: December 25, 2018
    Assignee: University of Central Florida Research Foundation, Inc.
    Inventors: James J. Hickman, Mainak Das, John W. Rumsey
  • METHOD FOR CULTURING SKELETAL MUSCLE FOR TISSUE ENGINEERING
    Publication number: 20160068812
    Abstract: The invention provides a nutrient medium composition and associated methods for lengthening the useful life of a culture of muscle cells. Disclosed is a method of culturing mammalian muscle cells, including preparing one or more carriers coated with a covalently bonded monolayer of trimethoxy-silylpropyl-diethylenetriamine (DETA); verifying DETA monolayer formation by one or more associated optical parameters; suspending isolated fetal rat skeletal muscle cells in serum-free medium according to medium composition 1; plating the suspended cells onto the prepared carriers at a predetermined density; leaving the carriers undisturbed for cells to adhere to the DETA monolayer; covering the carriers with a mixture of medium 1 and medium 2; and incubating.
    Type: Application
    Filed: October 19, 2015
    Publication date: March 10, 2016
    Inventors: James J. Hickman, Mainak Das, John W. Rumsey
  • Method for culturing skeletal muscle for tissue engineering
    Patent number: 9163216
    Abstract: The invention provides a nutrient medium composition and associated methods for lengthening the useful life of a culture of muscle cells. Disclosed is a method of culturing mammalian muscle cells, including preparing one or more carriers coated with a covalently bonded monolayer of trimethoxy-silylpropyl-diethylenetriamine (DETA); verifying DETA monolayer formation by one or more associated optical parameters; suspending isolated fetal rat skeletal muscle cells in serum-free medium according to medium composition 1; plating the suspended cells onto the prepared carriers at a predetermined density; leaving the carriers undisturbed for cells to adhere to the DETA monolayer; covering the carriers with a mixture of medium 1 and medium 2; and incubating.
    Type: Grant
    Filed: April 22, 2010
    Date of Patent: October 20, 2015
    Assignee: University of Central Florida Research Foundation, Inc.
    Inventors: James J. Hickman, Mainak Das, John W. Rumsey