Abstract: The present invention is directed to the purification and commercialization of therapeutic polypeptides that have. In one aspect of the invention, a method of purifying Interferon ?-1b using size exclusion chromatography is provided. In another aspect of the invention, a method of purifying a polypeptide using size chromatography is provided. A third aspect provides a method of commercializing a polypeptide.

Abstract: A waveguide system for radiating sound waves. The system includes a low loss waveguide for transmitting sound waves, having walls are tapered so that said cross-sectional area of the exit end is less than the cross-sectional area of the inlet end. In a second aspect of the invention, a waveguide for radiating sound waves, has segments of length approximately equal to A ? ( y ) = A inlet ? [ 1 - 2 ? Y B + ( y B ) 2 ] where l is the effective length of said waveguide and n is a positive integer. The product of a first set of alternating segments is greater than the product of a second set of alternating segments, in one embodiment, by a factor of three. In a third aspect of the invention, the first two aspects are combined.