Patents by Inventor Johnathan S. Coursey

Johnathan S. Coursey has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Patent number: 10363558
    Abstract: The present invention relates to systems and methods for the real time processing of nucleic acid during polymerase chain reaction (PCR) and thermal melt applications. According to an aspect of the invention, a system for the rapid serial processing of multiple nucleic acid assays is provided. In one embodiment, the system includes, but is not limited to: a microfluidic cartridge having microfluidic (flow-through) channels, a fluorescence imaging system, a temperature measurement and control system; a pressure measurement and control system for applying variable pneumatic pressures to the microfluidic cartridge; a storage device for holding multiple reagents (e.g., a well-plate); a liquid handling system comprising at least one robotic pipettor for aspirating, mixing, and dispensing reagent mixtures to the microfluidic cartridge; systems for data storage, processing, and output; and a system controller to coordinate the various devices and functions.
    Type: Grant
    Filed: August 24, 2015
    Date of Patent: July 30, 2019
    Assignee: Canon U.S. Life Sciences, Inc.
    Inventors: Ivor T. Knight, Kenton C. Hasson, Johnathan S. Coursey, Hongye Liang, Sami Kanderian, Gregory H. Owen, Weidong Cao, Ying-Xin Wang, Scott Corey, Ben Lane, Conrad Laskowski, Alex Flamm, Brian Murphy, Eric Schneider, Takayoshi Hanagata, Hiroshi Inoue, Shulin Zeng, Brian Bean, Franklin Regan
  • Publication number: 20180193839
    Abstract: The present invention, in one aspect, provides methods and systems for controlling slugs using temperature dependent fluorescent dyes. In some embodiments, the present invention uses one or more techniques to enhance the visibility of slugs, enhance a system's ability to differentiate between slugs, and enhance a system's ability to identify the positions of slugs.
    Type: Application
    Filed: January 5, 2018
    Publication date: July 12, 2018
    Applicant: Canon U.S. Life Sciences, Inc.
    Inventors: Johnathan S. Coursey, Kenton C. Hasson, Sami Kanderian, Gregory H. Owen, Hongye Liang, Scott Corey, Brian Bean
  • Publication number: 20180179602
    Abstract: Methods, devices, and systems for performing polymerase chain reaction (PCR) amplification and melt data acquisition according to a single slug approach in which a single slug in a microfluidic channel fills an entire thermal zone of the microfluidic channel, and the thermal zone used for both PCR temperature cycling and melt data acquisition. A detector may be configured to detect fluorescence from the thermal zone during the PCR temperature cycling for real-time PCR and/or during temperature ramping in the melt data acquisition. Slug position control may be achieved by detecting leading or trailing edges in a slug build target zone into which a slug passes after passing through the thermal zone. The single slug approach may break coupling between one or more events of the PCR amplification and melt data acquisition and enable events to be independently optimized.
    Type: Application
    Filed: February 26, 2018
    Publication date: June 28, 2018
    Applicant: Canon U.S. Life Sciences, Inc.
    Inventors: Johnathan S. Coursey, Kenton C. Hasson, Brian Bean, Scott Corey
  • Patent number: 9939336
    Abstract: The invention relates to systems and methods for calibrating and using resistance temperature detectors. In one embodiment, the system includes a calibration circuit comprising a resistance temperature detector in a bridge circuit with at least one potentiometer, and a programmable gain amplifier coupled to the bridge circuit. Embodiments of the invention further comprise methods for calibrating the bridge circuit and the programmable gain amplifier for use with the resistance temperature detector and methods for determining the self heating voltage of the bridge circuit.
    Type: Grant
    Filed: August 4, 2014
    Date of Patent: April 10, 2018
    Assignee: Canon U.S. Life Sciences, Inc.
    Inventors: Johnathan S. Coursey, Kenton C. Hasson, Gregory H. Owen
  • Patent number: 9919314
    Abstract: Systems and methods for air cooling a microfluidic device using confinement channels to isolate cooling air from exposed liquids are disclosed. The systems and methods may also thermally condition the cooling airflow for improved robustness of the microfluidic device. In one embodiment, the air cooling system includes a split-level cooling manifold including an inlet duct that directs cooling air to a microfluidic device and an outlet duct that directs air heated by the microfluidic device away from the microfluidic device. The temperature of cooling air may be measured. The cooling air may be preheated to a temperature that is higher than an expected ambient temperature. The temperature of the cooling air after being heated by a microfluidic device may be measured.
    Type: Grant
    Filed: October 26, 2015
    Date of Patent: March 20, 2018
    Assignee: Canon U.S. Life Sciences, Inc.
    Inventors: Johnathan S. Coursey, Kenton C. Hasson, Ben Lane, Eric Schneider
  • Patent number: 9903003
    Abstract: Methods, devices, and systems for performing polymerase chain reaction (PCR) amplification and melt data acquisition according to a single slug approach in which a single slug in a microfluidic channel fills an entire thermal zone of the microfluidic channel, and the thermal zone used for both PCR temperature cycling and melt data acquisition. A detector may be configured to detect fluorescence from the thermal zone during the PCR temperature cycling for real-time PCR and/or during temperature ramping in the melt data acquisition. Slug position control may be achieved by detecting leading or trailing edges in a slug build target zone into which a slug passes after passing through the thermal zone. The single slug approach may break coupling between one or more events of the PCR amplification and melt data acquisition and enable events to be independently optimized.
    Type: Grant
    Filed: March 15, 2013
    Date of Patent: February 27, 2018
    Assignee: Canon U.S. Life Sciences, Inc.
    Inventors: Johnathan S. Coursey, Kenton C. Hasson, Brian Bean, Scott Corey
  • Patent number: 9873122
    Abstract: The invention relates to methods and devices for control of an integrated thin-film device with a plurality of microfluidic channels. In one embodiment, a microfluidic device is provided that includes a microfluidic chip having a plurality of microfluidic channels and a plurality of multiplexed heater electrodes, wherein the heater electrodes are part of a multiplex circuit including a common lead connecting the heater electrodes to a power supply, each of the heater electrodes being associated with one of the microfluidic channels. The microfluidic device also includes a control system configured to regulate power applied to each heater electrode by varying a duty cycle, the control system being further configured to determine the temperature of each heater electrode by determining the resistance of each heater electrode.
    Type: Grant
    Filed: February 14, 2013
    Date of Patent: January 23, 2018
    Assignee: Canon U.S. Life Sciences, Inc.
    Inventor: Johnathan S. Coursey
  • Patent number: 9861985
    Abstract: The present invention, in one aspect, provides methods and systems for controlling slugs using temperature dependent fluorescent dyes. In some embodiments, the present invention uses one or more techniques to enhance the visibility of slugs, enhance a system's ability to differentiate between slugs, and enhance a system's ability to identify the positions of slugs.
    Type: Grant
    Filed: August 31, 2011
    Date of Patent: January 9, 2018
    Assignee: Canon U.S. Life Sciences, Inc.
    Inventors: Johnathan S. Coursey, Kenton C. Hasson, Sami Kanderian, Gregory H. Owen, Hongye Liang, Scott Corey, Brian Bean
  • Patent number: 9829389
    Abstract: The invention relates to methods and devices for control of an integrated thin-film device with a plurality of microfluidic channels. In one embodiment, a microfluidic device is provided that includes a microfluidic chip having a plurality of microfluidic channels and a plurality of multiplexed heater electrodes, wherein the heater electrodes are part of a multiplex circuit including a common lead connecting the heater electrodes to a power supply, each of the heater electrodes being associated with one of the microfluidic channels. The microfluidic device also includes a control system configured to regulate power applied to each heater electrode by varying a duty cycle, the control system being further configured to determine the temperature of each heater electrode by determining the resistance of each heater electrode.
    Type: Grant
    Filed: February 22, 2016
    Date of Patent: November 28, 2017
    Assignee: Canon U.S. Life Sciences, Inc.
    Inventors: Johnathan S. Coursey, Kenton C. Hasson, Gregory H. Owen, Gregory A. Dale
  • Publication number: 20170325288
    Abstract: The present invention relates to methods and systems that result in high quality, reproducible, thermal melt analysis on a microfluidic platform. The present invention relates to methods and systems using thermal systems including heat spreading devices, including interconnection methods and materials developed to connect heat spreaders to microfluidic devices. The present invention also relates to methods and systems for controlling, measuring, and calibrating the thermal systems of the present invention.
    Type: Application
    Filed: January 23, 2017
    Publication date: November 9, 2017
    Applicant: Canon U.S. Life Sciences, Inc.
    Inventors: Johnathan S. Coursey, Kenton C. Hasson
  • Patent number: 9766139
    Abstract: The present invention, in one aspect, provides systems and methods for using a single slug or multiple slugs containing one or more calibrators to determine a relationship between temperature and an electrical characteristic of the thermal sensor for use in connection with calibrating thermal sensors. In some embodiments, the present invention uses the described calibrator systems and methods to calibrate thermal control elements on a microfluidic device. In non-limiting embodiment, the calibrator can be one or more of droplets, plugs, slugs, segments or a continuous flow of any appropriate solution that, when heated, yields a thermal response profile with a plurality of features (e.g., maxima, minima, inflection points, linear regions, etc.).
    Type: Grant
    Filed: March 20, 2013
    Date of Patent: September 19, 2017
    Assignee: Canon U.S. Life Sciences, Inc.
    Inventors: Johnathan S. Coursey, Kenton C. Hasson
  • Patent number: 9701997
    Abstract: The present invention relates to compositions and methods for in-system priming of microfluidic devices. Aspects of the present invention relate to compositions and methods for in-system priming of microfluidic devices utilizing a priming solution comprising adding and/or increasing concentrations of surfactant in a buffer solution.
    Type: Grant
    Filed: June 22, 2015
    Date of Patent: July 11, 2017
    Assignee: Canon U.S. Life Sciences, Inc.
    Inventors: Ying-Xin Wang, Johnathan S. Coursey, Jason Schmidt, Kenton C. Hasson
  • Patent number: 9657331
    Abstract: The present invention relates to compositions and methods for in-system priming of microfluidic devices. Aspects of the present invention relate to compositions and methods for in-system priming of microfluidic devices utilizing a priming solution comprising adding and/or increasing concentrations of surfactant in a buffer solution.
    Type: Grant
    Filed: June 22, 2015
    Date of Patent: May 23, 2017
    Assignee: Canon U.S. Life Sciences, Inc.
    Inventors: Ying-Xin Wang, Johnathan S. Coursey, Jason Schmidt, Kenton C. Hasson
  • Publication number: 20170108384
    Abstract: The invention relates to methods and devices for control of an integrated thin-film device with a plurality of microfluidic channels. In one embodiment, a microfluidic device is provided that includes a microfluidic chip having a plurality of microfluidic channels and a plurality of multiplexed heater electrodes, wherein the heater electrodes are part of a multiplex circuit including a common lead connecting the heater electrodes to a power supply, each of the heater electrodes being associated with one of the microfluidic channels. The microfluidic device also includes a control system configured to regulate power applied to each heater electrode by varying a duty cycle, the control system being further configured to determine the temperature each heater electrode by determining the resistance of each heater electrode.
    Type: Application
    Filed: December 23, 2016
    Publication date: April 20, 2017
    Applicant: Canon U.S. Life Sciences, Inc.
    Inventors: Kenton C. Hasson, Johnathan S. Coursey, Gregory H. Owen, Gregory A. Dale
  • Publication number: 20170067784
    Abstract: The invention relates to methods and devices for control of an integrated thin-film device with a plurality of microfluidic channels. In one embodiment, a microfluidic device is provided that includes a microfluidic chip having a plurality of microfluidic channels and a plurality of multiplexed heater electrodes, wherein the heater electrodes are part of a multiplex circuit including a common lead connecting the heater electrodes to a power supply, each of the heater electrodes being associated with one of the microfluidic channels. The microfluidic device also includes a control system configured to regulate power applied to each heater electrode by varying a duty cycle, the control system being further configured to determine the temperature each heater electrode by determining the resistance of each heater electrode.
    Type: Application
    Filed: November 18, 2016
    Publication date: March 9, 2017
    Applicant: Canon U.S. Life Sciences, Inc.
    Inventors: Kenton C. Hasson, Johnathan S. Coursey, Gregory H. Owen, Gregory A. Dale
  • Patent number: 9554422
    Abstract: The present invention relates to methods and systems that result in high quality, reproducible, thermal melt analysis on a microfluidic platform. The present invention relates to methods and systems using thermal systems including heat spreading devices, including interconnection methods and materials developed to connect heat spreaders to microfluidic devices. The present invention also relates to methods and systems for controlling, measuring, and calibrating the thermal systems of the present invention.
    Type: Grant
    Filed: May 17, 2012
    Date of Patent: January 24, 2017
    Assignee: Canon U.S. Life Sciences, Inc.
    Inventors: Johnathan S. Coursey, Kenton C. Hasson
  • Patent number: 9527083
    Abstract: The invention relates to methods and devices for control of an integrated thin-film device with a plurality of microfluidic channels. In one embodiment, a microfluidic device is provided that includes a microfluidic chip having a plurality of microfluidic channels and a plurality of multiplexed heater electrodes, wherein the heater electrodes are part of a multiplex circuit including a common lead connecting the heater electrodes to a power supply, each of the heater electrodes being associated with one of the microfluidic channels. The microfluidic device also includes a control system configured to regulate power applied to each heater electrode by varying a duty cycle, the control system being further configured to determine the temperature each heater electrode by determining the resistance of each heater electrode.
    Type: Grant
    Filed: June 30, 2008
    Date of Patent: December 27, 2016
    Assignee: Canon U.S. Life Sciences, Inc.
    Inventors: Kenton C. Hasson, Johnathan S. Coursey, Gregory H. Owen, Gregory A. Dale
  • Publication number: 20160341605
    Abstract: The invention relates to methods and devices for control of an integrated thin-film device with a plurality of microfluidic channels. In one embodiment, a microfluidic device is provided that includes a microfluidic chip having a plurality of microfluidic channels and a plurality of multiplexed heater electrodes, wherein the heater electrodes are part of a multiplex circuit including a common lead connecting the heater electrodes to a power supply, each of the heater electrodes being associated with one of the microfluidic channels. The microfluidic device also includes a control system configured to regulate power applied to each heater electrode by varying a duty cycle, the control system being further configured to determine the temperature of each heater electrode by determining the resistance of each heater electrode.
    Type: Application
    Filed: February 22, 2016
    Publication date: November 24, 2016
    Applicant: Canon U.S. Life Sciences, Inc.
    Inventors: Johnathan S. Coursey, Kenton C. Hasson, Gregory H. Owen, Gregory A. Dale
  • Publication number: 20160051985
    Abstract: The present invention relates to systems and methods for the real time processing of nucleic acid during polymerase chain reaction (PCR) and thermal melt applications. According to an aspect of the invention, a system for the rapid serial processing of multiple nucleic acid assays is provided. In one embodiment, the system includes, but is not limited to: a microfluidic cartridge having microfluidic (flow-through) channels, a fluorescence imaging system, a temperature measurement and control system; a pressure measurement and control system for applying variable pneumatic pressures to the microfluidic cartridge; a storage device for holding multiple reagents (e.g., a well-plate); a liquid handling system comprising at least one robotic pipettor for aspirating, mixing, and dispensing reagent mixtures to the microfluidic cartridge; systems for data storage, processing, and output; and a system controller to coordinate the various devices and functions.
    Type: Application
    Filed: August 24, 2015
    Publication date: February 25, 2016
    Applicant: Canon U.S. Life Sciences, Inc.
    Inventors: Ivor T. KNIGHT, Kenton C. Hasson, Johnathan S. Coursey, Hongye Liang, Sami Kanderian, Gregory H. Owen, Weidong Cao, Ying-Xin Wang, Scott Corey, Ben Lane, Conrad Laskowski, Alex Flamm, Brian Murphy, Eric Schneider, Takayoshi Hanagata, Hiroshi Inoue, Shulin Zeng, Brian Bean, Franklin Regan
  • Patent number: 9267852
    Abstract: The invention relates to methods and devices for control of an integrated thin-film device with a plurality of microfluidic channels. In one embodiment, a microfluidic device is provided that includes a microfluidic chip having a plurality of microfluidic channels and a plurality of multiplexed heater electrodes, wherein the heater electrodes are part of a multiplex circuit including a common lead connecting the heater electrodes to a power supply, each of the heater electrodes being associated with one of the microfluidic channels. The microfluidic device also includes a control system configured to regulate power applied to each heater electrode by varying a duty cycle, the control system being further configured to determine the temperature of each heater electrode by determining the resistance of each heater electrode.
    Type: Grant
    Filed: November 5, 2012
    Date of Patent: February 23, 2016
    Assignee: Canon U.S. Life Sciences, Inc.
    Inventors: Johnathan S. Coursey, Kenton C. Hasson, Gregory H. Owen, Gregory A Dale