Abstract: Five fluorogenic probe hydrolysis reverse transcriptase-polymerase chain reaction (RT-PCR) (TaqMan™) assays are described for serotype-specific detection of dengue 1–4 and group-specific detection of dengue viruses. Type- and group-specific oligonucleotide primers and fluorogenic probes were designed against conserved regions of the dengue genome. The invention provides TaqMan PCR assays, which are rapid, sensitive, and specific screening and serotyping tools for the epidemiological study of dengue infections.

Abstract: Five fluorogenic probe hydrolysis reverse transcriptase-polymerase chain reaction (RT-PCR) (TaqMan™) assays are described for serotype-specific detection of dengue 1-4 and group-specific detection of dengue viruses. Type- and group-specific oligonucleotide primers and fluorogenic probes were designed against conserved regions of the dengue genome. The invention provides TaqMan PCR assays, which are rapid, sensitive, and specific screening and serotyping tools for the epidemiological study of dengue infections.

Abstract: Five fluorogenic probe hydrolysis reverse transcriptase-polymerase chain reaction (RT-PCR) (TaqMan™) assays are described for serotype-specific detection of dengue 1-4 and group-specific detection of dengue viruses. Type- and group-specific oligonucleotide primers and fluorogenic probes were designed against conserved regions of the dengue genome. The invention provides TaqMan PCR assays, which are rapid, sensitive, and specific screening and serotyping tools for the epidemiological study of dengue infections.

Abstract: The invention relates to diagnostic methods, probes, detection systems and kits for the identification of foot and mouth disease virus (FMDV) infection in a biological sample obtained from a farm animal. It was discovered that a highly conserved region of sequence existed with the 3D coding region of the FMDV genome. This region was found to be strikingly similar, and often identical or with only one or two nucleotide substitution, between the various serotypes of FMDV. Thus, by performing PCR analysis with probes comprising sequences form this region or ELISA with antibodies directed to polypeptide products expressed from this region, a plurality of serotypes of FMDV could be detected from a single test. Further, by including dried PCR reagents plus trehelose, kits could be stored at room temperatures for long periods of time without any significant loss in sensitivity or specificity.