Patents by Inventor Jon A. Hoshizaki
Jon A. Hoshizaki has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
-
Publication number: 20140272980Abstract: A method of sensing nucleotide reactions includes flowing at least one nucleotide solution from a container of at least two containers of a sensor system. The sensor system includes a sensor sensitive to a byproduct of nucleotide incorporation. Each container of the at least two containers includes a different nucleotide solution. The sensor system enters an idle mode after flowing. The method further includes cycling the at least two containers through at least two cycles. Each cycle includes depressurizing the at least two containers for a first period and pressurizing the at least two containers for a second period. The method also includes pressurizing the at least two containers when the sensor system enters an active mode.Type: ApplicationFiled: March 13, 2013Publication date: September 18, 2014Applicant: LIFE TECHNOLOGIES CORPORATIONInventors: James Ball, David Kupec, David Marran, Jon A. Hoshizaki
-
Patent number: 8602958Abstract: Assemblies for and methods of coupling a microtiter plate and receptacle for centrifugation of liquid from the microtiter plate to the receptacle are provided. In some embodiments, a coupling frame can be used. In other embodiments, the microtiter plate couples directly to the receptacle. In some embodiments, relative motion between the receptacle and the microtiter plate is limited in the x-y plane. In some embodiments, relative motion between the receptacle and the microtiter plate is limited in the x-z plane. In some embodiments, relative motion between the receptacle and the microtiter plate is limited in the y-z plane.Type: GrantFiled: November 26, 2008Date of Patent: December 10, 2013Assignee: Life Technologies CorporationInventors: Patrick D. Kinney, Michele E. Wisniewski, Jon A. Hoshizaki, David M. Liu, Joon Mo Yang
-
Publication number: 20130288873Abstract: An apparatus includes a robotic system providing movement in three orthogonal directions to an arm operable to receive a pipette tip and to facilitate movement of fluid into and out of the pipette tip. In addition, the apparatus can include a tray for receiving pipette tips, receptacles for receiving tubes, an apparatus for forming an emulsion, a device for forming particles that include copies of the polynucleotide, a device for enriching the particles and an apparatus for loading such particles onto a sensor array. The apparatus can further include receptacles for holding containers of reagent solutions. Optionally, the robot can include a gripper arm in addition to the pipette receiving arm.Type: ApplicationFiled: March 14, 2013Publication date: October 31, 2013Applicant: LIFE TECHNOLOGIES CORPORATIONInventors: Kristopher BARBEE, Ryan Jones, Sean McCusker, Maximilan Carpino, John Leamon, Jonathan Schultz, Jon A. Hoshizaki
-
Publication number: 20110128545Abstract: Various embodiments of a sequencing system capable of rapidly imaging samples at multiple wavelengths are provided herein. In one embodiment, the system includes a fast-indexing filter wheel having a plurality of excitation and emission filters capable of being rapidly rotated into and out of communication with an excitation source (e.g., an arc lamp, a laser. For example, the filter wheel can be configured to index in an amount of time falling within a range of about 40 ms to about 60 ms, preferably 50 ms. The system can also be configured to account for vibrations resulting from the quick starts and stops of the fast-indexing filter wheel as well as vibrations resulting from other sources. Various methods of rapidly imaging a sample at multiple wavelengths are also provided herein.Type: ApplicationFiled: August 31, 2010Publication date: June 2, 2011Applicant: LIFE TECHNOLOGIES CORPORATIONInventors: David Cox, Marc Haberstroh, Patrick Kinney, Albert Carrillo, Jon Hoshizaki, Maryam Shariati, Howard King, Joe Lee, Matthew Chan
-
Publication number: 20110124111Abstract: Various embodiments of a low-volume sequencing system are provided herein. The system can include a low-volume flowcell having at least one reaction chamber of a defined volume (e.g., less than about 100 ?l). The system can also include an automated reagent delivery mechanism configured to reversibly couple with the inlet port corresponding to a target reaction chamber thereby placing allowing for reagent to be accurately moved from a storage container to the reaction chamber with minimal reagent waste. The flowcells can include a plurality of reaction chambers (e.g., 6) thereby allowing for parallel analysis of multiple samples. Various methods of analyzing a biomolecule are also provided herein.Type: ApplicationFiled: August 31, 2010Publication date: May 26, 2011Applicant: LIFE TECHNOLOGIES CORPORATIONInventors: Jon HOSHIZAKI, Joon Mo YANG, Maryam SHARIATI, David COX, Kirk HIRANO, John BRIDGHAM, Sam WOO, George GOLDA
-
Patent number: 7599060Abstract: An optical system includes a sample substrate having a surface, the surface defining a 2-dimensional sample plane. The system includes an excitation source configured to provide excitation light to the sample substrate. The system further includes an optical detector configured to receive emission light from the sample substrate and generate detection data. The system also includes a scan head configured at least (i) to direct the excitation light towards the sample substrate, (ii) to receive emission light from the sample substrate and direct the emission light towards the optical detector, and (iii) for scanning relative to the sample substrate.Type: GrantFiled: August 18, 2008Date of Patent: October 6, 2009Assignee: Applied Biosystems, LLCInventors: Jon A. Hoshizaki, Howard G. King, Johannes P. Sluis, Steven J. Boege, Mark F. Oldham
-
Publication number: 20080316482Abstract: Methods and optical systems for scanning of a target sample, including methods and systems using a low mass scan head. The present invention also relates to methods and systems for performing sample assays, and for producing and measuring optical responses and signatures.Type: ApplicationFiled: August 18, 2008Publication date: December 25, 2008Inventors: Jon A. Hoshizaki, Howard Gregg King, Johannes P. Sluis, Steven J. Boege, Mark F. Oldham
-
Patent number: 7452510Abstract: A multi-well microfiltration apparatus and method are provided and feature a manual touch-off system for transferring pendent drops hanging from discharge-conduits of a discharge-conduit array to respective receiving wells or receiving holes of a corresponding receiving array, with minimum or no cross-contamination between the discharge conduits, or the receiving wells or receiving holes. The manual touch-off is achieved by manually shifting a carriage that supports one of the arrays, into a position whereat pendent drops of fluid hanging from the distal ends of the discharge conduits contact the inner sidewalls of the corresponding receiving wells or receiving holes of the receiving array.Type: GrantFiled: January 24, 2006Date of Patent: November 18, 2008Assignee: Applied Biosystems Inc.Inventors: Todd A. Weinfield, Gary Lim, Donald R. Sandell, Kevin S. Bodner, Mark Borodkin, Mark Oldham, Jon Hoshizaki
-
Patent number: 7423750Abstract: Methods and optical systems for scanning of a target sample, including methods and systems using a low mass scan head and methods and systems for conducting a scanned optically transduced assay where the scanning includes at least one first relative angular motion and at least one second angular motion or at least one linear motion. The present invention also relates to methods and systems for performing sample assays, and for producing and measuring optical responses and signatures.Type: GrantFiled: November 3, 2005Date of Patent: September 9, 2008Assignee: Applera CorporationInventors: Jon A. Hoshizaki, Howard Gregg King, Johannes P. Sluis, Steven J. Boege, Mark F. Oldham
-
Patent number: 7295316Abstract: A fluorometry device and method adapted to determine concentration of spectrally distinguishable species in a biological sample with a plurality of movable optical devices.Type: GrantFiled: January 14, 2004Date of Patent: November 13, 2007Assignee: Applera CorporationInventors: Steven J. Boege, Jon A. Hoshizaki, Mark F. Oldham, Liana Ilkova
-
Publication number: 20060191893Abstract: A multi-well microfiltration apparatus and method are provided and feature a manual touch-off system for transferring pendent drops hanging from discharge-conduits of a discharge-conduit array to respective receiving wells or receiving holes of a corresponding receiving array, with minimum or no cross-contamination between the discharge conduits, or the receiving wells or receiving holes. The manual touch-off is achieved by manually shifting a carriage that supports one of the arrays, into a position whereat pendent drops of fluid hanging from the distal ends of the discharge conduits contact the inner sidewalls of the corresponding receiving wells or receiving holes of the receiving array.Type: ApplicationFiled: January 24, 2006Publication date: August 31, 2006Applicant: Applera CorporationInventors: Todd Weinfield, Gary Lim, Donald Sandell, Kevin Bodner, Mark Borodkin, Mark Oldham, Jon Hoshizaki
-
Publication number: 20060121602Abstract: Methods and optical systems for scanning of a target sample, including methods and systems using a low mass scan head. The present invention also relates to methods and systems for performing sample assays, and for producing and measuring optical responses and signatures.Type: ApplicationFiled: November 3, 2005Publication date: June 8, 2006Inventors: Jon Hoshizaki, Howard King, Johannes Sluis, Steven Boege, Mark Oldham
-
Patent number: 7019267Abstract: A heater module is described that includes a heat distribution plate including a bottom portion having first and second sides and a plurality of projections extending away from one of the sides. A heat source is provided for heating the heat distribution plate, and, optionally, a heating tray can be used to receive the heat source and heat distribution plate. The heater module is adapted to engage a sample purification tray having a plurality of purification and/or discharge columns which can extend through openings in the heater module and direct a sample into a sample receiving tray. Methods of heating samples using the heater module are also described.Type: GrantFiled: May 3, 2005Date of Patent: March 28, 2006Assignee: Applera CorporationInventors: Todd A. Weinfield, Gary Lim, Donald R. Sandell, Kevin S. Bodner, Mark Borodkin, Mark Oldham, Jon Hoshizaki
-
Publication number: 20050194371Abstract: A heater module is described that includes a heat distribution plate including a bottom portion having first and second sides and a plurality of projections extending away from one of the sides. A heat source is provided for heating the heat distribution plate, and, optionally, a heating tray can be used to receive the heat source and heat distribution plate. The heater module is adapted to engage a sample purification tray having a plurality of purification and/or discharge columns which can extend through openings in the heater module and direct a sample into a sample receiving tray. Methods of heating samples using the heater module are also described.Type: ApplicationFiled: May 3, 2005Publication date: September 8, 2005Applicant: Applera CorporationInventors: Todd Weinfield, Gary Lim, Donald Sandell, Kevin Bodner, Mark Borodkin, Mark Oldham, Jon Hoshizaki
-
Publication number: 20050151972Abstract: A fluorometry device and method adapted to determine concentration of spectrally distinguishable species in a biological sample with a plurality of movable optical devices.Type: ApplicationFiled: January 14, 2004Publication date: July 14, 2005Applicant: Applera CorporationInventors: Steven Boege, Jon Hoshizaki, Mark Oldham, Liana Ilkova
-
Patent number: 6906292Abstract: A heater module is described that includes a heat distribution plate including a bottom portion having first and second sides and a plurality of projections extending away from one of the sides. A heat source is provided for heating the heat distribution plate, and, optionally, a heating tray can be used to receive the heat source and heat distribution plate. The heater module is adapted to engage a sample purification tray having a plurality of purification and/or discharge columns which can extend through openings in the heater module and direct a sample into a sample receiving tray. Methods of heating samples using the heater module are also described.Type: GrantFiled: February 6, 2003Date of Patent: June 14, 2005Assignee: Applera CorporationInventors: Todd A. Weinfield, Gary Lim, Donald R. Sandell, Kevin S. Bodner, Mark Borodkin, Mark Oldham, Jon Hoshizaki
-
Patent number: 6896849Abstract: A multi-well microfiltration apparatus and method are provided and feature a manual touch-off system for transferring pendent drops hanging from discharge-conduits of a discharge-conduit array to respective receiving wells or receiving holes of a corresponding receiving array, with minimum or no cross-contamination between the discharge conduits, or the receiving wells or receiving holes. The manual touch-off is achieved by manually shifting a carriage that supports one of the arrays, into a position whereat pendent drops of fluid hanging from the distal ends of the discharge conduits contact the inner sidewalls of the corresponding receiving wells or receiving holes of the receiving array.Type: GrantFiled: March 22, 2002Date of Patent: May 24, 2005Assignee: Applera CorporationInventors: Mark T. Reed, Jon Hoshizaki
-
Patent number: 6783732Abstract: The present invention provides multi-well plates and column arrays in which samples (e.g., cell lysates containing nucleic acids of interest, such as RNA) can be analyzed and/or processed. In one embodiment, the microfiltration arrangement is a multilayer structure, including (i) a column plate having an array of minicolumns into which samples can be placed, (ii) a discrete filter element disposed in each minicolumn, (iii) a drip-director plate having a corresponding array of drip directors through which filtrate may egress, and (iv) a receiving-well plate having a corresponding array of receiving wells into which filtrate can flow. The invention provides multi-well microfiltration arrangements that are relatively simple to manufacture and that overcome many of the problems associated with the prior arrangements relating to (i) cross-contamination due to wicking across a common filter sheet or (ii) individual filter elements entrapping sample constituents within substantial dead volumes.Type: GrantFiled: July 19, 2002Date of Patent: August 31, 2004Assignee: Applera CorporationInventors: Alfred P Madden, Jon Hoshizaki, Jason H. Halsey, Stephen E. Moring, Mark F. Oldham, Kevin S. Bodner
-
Publication number: 20040033619Abstract: A heater module is described that includes a heat distribution plate including a bottom portion having first and second sides and a plurality of projections extending away from one of the sides. A heat source is provided for heating the heat distribution plate, and, optionally, a heating tray can be used to receive the heat source and heat distribution plate. The heater module is adapted to engage a sample purification tray having a plurality of purification and/or discharge columns which can extend through openings in the heater module and direct a sample into a sample receiving tray. Methods of heating samples using the heater module are also described.Type: ApplicationFiled: February 6, 2003Publication date: February 19, 2004Inventors: Todd A. Weinfield, Gary Lim, Donald R. Sandell, Kevin S. Bodner, Mark Borodkin, Mark Oldham, Jon Hoshizaki
-
Patent number: 6506343Abstract: The present invention provides multi-well plates and column arrays in which samples (e.g., cell lysates containing nucleic acids of interest, such as RNA) can be analyzed and/or processed. In one embodiment, the microfiltration arrangement is a multilayer structure, including (i) a column plate having an array of minicolumns into which samples can be placed, (ii) a discrete filter element disposed in each minicolumn, (iii) a drip-director plate having a corresponding array of drip directors through which filtrate may egress, and (iv) a receiving-well plate having a corresponding array of receiving wells into which filtrate can flow. The invention provides multi-well microfiltration arrangements that are relatively simple to manufacture and that overcome many of the problems associated with the prior arrangements relating to (i) cross-contamination due to wicking across a common filter sheet or (ii) individual filter elements entrapping sample constituents within substantial dead volumes.Type: GrantFiled: May 4, 2000Date of Patent: January 14, 2003Assignee: Applera CorporationInventors: Kevin S. Bodner, Alfred P. Madden, Jason H. Halsey, Mark F. Oldham, Stephen E. Moring, Jon Hoshizaki