Abstract: A method for extracting ATP from a biological sample is disclosed. The method involves introducing a cationic extractant and an anionic substance and then extracting ATP. The method may be used to assay for the presence of ATP in a biological sample or to determine the amount of ATP extracted from a biological sample. The method is particularly useful in detecting contamination on surfaces and in food products. A reagent, a test device and a test kit that involve the use of the method to detect contamination are also disclosed.

Abstract: A method for extracting ATP from a biological sample is disclosed. The method involves introducing a cationic extractant and an anionic substance and then extracting ATP. The method may be used to assay for the presence of ATP in a biological sample or to determine the amount of ATP extracted from a biological sample. The method is particularly useful in detecting contamination on surfaces and in food products. A reagent, a test device and a test kit that involve the use of the method to detect contamination are also disclosed.

Abstract: Apparatus for performing and measuring chemical reactions includes a reaction test apparatus having reaction wells wherein reactants are controllably mixed, and exposure apparatus which receives and positions the reaction test apparatus adjacent a photographic film. Each of the reaction wells includes at least two reaction chambers, arranged in a side by side fashion. All but the final reaction chamber have upwardly sloping sides, so that liquid placed in one reaction chamber can flow to the next reaction chamber when the apparatus is tilted. In a preferred embodiment, the reaction wells are supported in a plate that is structurally integral with the wells but separates the wells from each other. The test plate is retained in the exposure apparatus, and liquid is controllably flowed from one reaction chamber to the next by tilting the exposure apparatus.

Abstract: At least some of the reagents required for a chemiluminescent reaction are dried on a piece of a carrier material. The reagents are stable for extended periods of time, without refrigeration, in this form. The sheet of carrier material is of a shape and size to fit flat against the interior surface of a transparent wall of a test well through which light output of the reaction is measured. Light output is thereby concentrated in a region adjacent the wall, minimizing attenuation of the light in passing from the well to permit measurement of low light output levels.

Abstract: Apparatus for performing and measuring chemical reactions includes a reaction test apparatus having reaction wells wherein reactants are controllably mixed, and exposure apparatus which receives and positions the reaction test apparatus adjacent a photographic film. Each of the reaction wells includes at least two reaction cups, arranged one above the other. The uppermost reaction cups have orifices in the bottoms, so that liquid can be mixed and reacted in the uppermost cup, and then controllably transferred to the lower cup to be mixed with additional reactants. In a preferred embodiment, the reaction cups are supported in plates that are structurally integrated with the cups, and are superimposed to make a test block. The test block is retained in the exposure apparatus, and liquid is forced from the upper cup to the lower cup by application of pressure to the top of the upper cup.

Abstract: Lecithin is coated onto the surface of plastics such as polystyrene and polyethylene to enhance the wettability of aqueous liquids to the plastic. In one application, plastic test wells are coated with lecithin to enhance the ability of liquids placed into a well with sloping sides to flow in a regular manner into a well with vertical sides. The lecithin is applied to the plastic by dissolving the lecithin in an organic solvent such as heptane, coating the solution onto the plastic surface, and evaporating the solvent.

Abstract: Apparatus for performing and measuring chemical reactions includes a reaction test apparatus having reaction wells wherein reactants are controllably mixed, and exposure apparatus which receives and positions the reaction test apparatus adjacent a photographic film. Each of the reaction wells includes at least two reaction cups, arranged one above the other. The uppermost reaction cups have orifices in the bottoms, so that liquid can be mixed and reacted in the uppermost cup, and then controllably transferred to the lower cup to be mixed with additional reactants. In a preferred embodiment, the reaction cups are supported in plates that are structurally integral with the cups, and are superimposed to make a test block. The test block is retained in the exposure apparatus, and liquid is forced from the upper cup to the lower cup by application of pressure to the top of the upper cup.

Abstract: Apparatus for performing and measuring chemical reactions includes a reaction test apparatus having reaction wells wherein reactants are controllably mixed, and exposure apparatus which receives and positions the reaction test apparatus adjacent a photographic film. Each of the reaction wells includes at least two reaction chambers, arranged in a side by side fashion. All but the final reaction chamber have upwardly sloping sides, so that liquid placed in one reaction chamber can flow to the next reaction chamber when the apparatus is tilted. In a preferred embodiment, the reaction wells are supported in a plate that is structurally integral with the wells but separates the wells from each other. The test plate is retained in the exposure apparatus, and liquid is controllably flowed from one reaction chamber to the next by tilting the exposure apparatus.