Patents by Inventor Jon G. Wilkes
Jon G. Wilkes has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 9194868Abstract: In various embodiments, the present disclosure describes methods and systems for detecting microbes in a sample. The methods are generally applicable to quantifying the number of target bacteria in a sample counted from a detection region of a flow cytometer histogram. The detection methods can be employed in the presence of other microorganisms and other non-target microbe components to selectively quantify the amount of a target microbe. The methods are advantageous over those presently existing for testing of foodstuffs and diagnostic evaluation in their speed, accuracy and ease of use. Various swab collection devices and kits useful for practicing the present disclosure are also described herein.Type: GrantFiled: August 17, 2009Date of Patent: November 24, 2015Assignees: The United States of America, Vivione Biosciences, LLCInventors: Dan A. Buzatu, Jon G. Wilkes, Ted A. Moskal, Bill Nevius, Jason T. Taylor, Randal K. Tucker, Melinda Miller, Shawn Ramsanoop
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Patent number: 9103788Abstract: Methods for detecting one or more target bacteria in a test sample are provided. It is shown herein that photosensitizers combined with intense light exposure reduce fluorescing background due to non-bacterial particles. This permits detection of subsequently labeled target bacterial cells (e.g., using a fluorescently labeled antibody) against a largely black background. In particular examples, the methods include incubating the test sample in a growth medium that permits growth of bacteria present in the sample, contacting the sample with a photo-sensitizer; exposing the sample to light under conditions sufficient for the photo-sensitizer to photobleach contaminating non-bacterial particulates present in the sample. The bacteria can then be substantially separated from the sample, thereby generating an isolated bacterial sample. The method can also include contacting the isolated bacterial sample with a binding agent specific for the one or more target bacteria, and detecting the one or more target bacteria.Type: GrantFiled: November 30, 2012Date of Patent: August 11, 2015Assignees: THE UNITED STATES OF AMERICA, AS REPRESENTED BY THE SECRETARY, DEPARTMENT OF HEALTH AND HUMAN SERVICES, VIVIONE BIOSCIENCES, LLCInventors: Jon G. Wilkes, Dan Buzatu, Randal Tucker, Thaddeus John Moskal, Jr.
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Publication number: 20150065853Abstract: A system and method for preprocessing magnetic resonance spectroscopy (MRS) data of brain tissue for pattern-based diagnostics is disclosed. The MRS preprocessing system includes an MRS preprocessing module that executes an operation that normalizes MRS spectrum data, recalibrates and scales the normalized MRS spectrum data, and then renormalizes the scaled MRS spectrum data. The resulting preprocessed MRS data is used to assist in identifying abnormalities in tissues shown in MRS scans.Type: ApplicationFiled: September 5, 2014Publication date: March 5, 2015Applicant: The United States of America, as represented by the Secretary, Dept. of Health and Human ServicesInventors: Jon G. Wilkes, Dan A. Buzatu, Pierre Alusta, Bruce Pearce, Ryan M. Kretzer, Inessa IM, Richard D. Beger
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Patent number: 8880354Abstract: A system and method for preprocessing magnetic resonance spectroscopy (MRS) data of brain tissue for pattern-based diagnostics is disclosed. The MRS preprocessing system includes an MRS preprocessing module that executes an operation that normalizes MRS spectrum data, recalibrates and scales the normalized MRS spectrum data, and then renormalizes the scaled MRS spectrum data. The resulting preprocessed MRS data is used to assist in identifying abnormalities in tissues shown in MRS scans.Type: GrantFiled: November 12, 2010Date of Patent: November 4, 2014Assignee: The United States of America, as Represented by the Secretary, Department of Health and Human ServicesInventors: Jon G. Wilkes, Dan A. Buzatu, Pierre Alusta, Bruce Pearce, Ryan M. Kretzer, Inessa Im, Richard D. Beger
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Patent number: 8704169Abstract: Disclosed is a mass spectrometer for analyzing a sample that has or is suspected of having microorganisms. The disclosed mass spectrometer has been uniquely configured to include a sample platform which functions as a counter electrode or discharge electrode and a surface to provide the sample to be analyzed. The mass spectrometer also includes an ion source positioned adjacent to the sample platform for ionizing and volatizing molecules within the sample, wherein the sample platform and the ion source are positioned such that during operation of the mass spectrometer an electrical discharge takes place between the ion source and the sample platform. Also disclosed are methods for generating a mass spectrum profile/fingerprint of a sample. The methods include positioning a sample platform having a sample adjacent to an ion source.Type: GrantFiled: October 11, 2011Date of Patent: April 22, 2014Assignee: The United States of America, as represented by the Secretary, Department of Health and Human ServicesInventors: Pierre Alusta, Cameron Dorey, William Ryan Parker, Jon G. Wilkes, Dan Buzatu
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Publication number: 20130131992Abstract: A system and method for preprocessing magnetic resonance spectroscopy (MRS) data of brain tissue for pattern-based diagnostics is disclosed. The MRS preprocessing system includes an MRS preprocessing module that executes an operation that normalizes MRS spectrum data, recalibrates and scales the normalized MRS spectrum data, and then renormalizes the scaled MRS spectrum data. The resulting preprocessed MRS data is used to assist in identifying abnormalities in tissues shown in MRS scans.Type: ApplicationFiled: November 12, 2010Publication date: May 23, 2013Applicant: The Government USA as Represented by the Secretary Dept. of Health and Human ServicesInventors: Jon G. Wilkes, Dan A. Buzatu, Pierre Alusta, Bruce Pearce, Ryan M. Kretzer, Inessa Im, Richard D. Beger
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Publication number: 20130087700Abstract: Disclosed is a mass spectrometer for analyzing a sample that has or is suspected of having microorganisms. The disclosed mass spectrometer has been uniquely configured to include a sample platform which functions as a counter electrode or discharge electrode and a surface to provide the sample to be analyzed. The mass spectrometer also includes an ion source positioned adjacent to the sample platform for ionizing and volatizing molecules within the sample, wherein the sample platform and the ion source are positioned such that during operation of the mass spectrometer an electrical discharge takes place between the ion source and the sample platform. Also disclosed are methods for generating a mass spectrum profile/fingerprint of a sample. The methods include positioning a sample platform having a sample adjacent to an ion source.Type: ApplicationFiled: October 11, 2011Publication date: April 11, 2013Inventors: Pierre Alusta, Cameron Dorey, William Ryan Parker, Jon G. Wilkes, Dan Buzatu
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Publication number: 20110217694Abstract: In various embodiments, the present disclosure describes methods and systems for detecting microbes in a sample. The methods are generally applicable to quantifying the number of target bacteria in a sample counted from a detection region of a flow cytometer histogram. The detection methods can be employed in the presence of other microorganisms and other non-target microbe components to selectively quantify the amount of a target microbe. The methods are advantageous over those presently existing for testing of foodstuffs and diagnostic evaluation in their speed, accuracy and ease of use. Various swab collection devices and kits useful for practicing the present disclosure are also described herein.Type: ApplicationFiled: August 17, 2009Publication date: September 8, 2011Inventors: Dan A. Buzatu, Jon G. Wilkes, Ted A. Moskal, Bill Nevius, Jason T. Taylor, Randal K. Tucker, Melinda Miller, Shawn Ramsanoop
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Patent number: 7996156Abstract: Structure-activity methods based on molecular descriptors that are a combination of structural information about the through-space and through-bond relationships between components of a molecule's structure and spectral data attributable to those components are disclosed. In some embodiments, a molecule is described by multiple sets of such descriptors to account for flexibility in the structure of the molecule. In a particularly disclosed embodiment, predicted 13C—13C COSY data and 13C—13C distance data are used as descriptors. Models of molecular properties may be established using the disclosed spectral data-activity methods and used to predict the properties of molecules.Type: GrantFiled: March 7, 2003Date of Patent: August 9, 2011Assignee: The United States of America as represented by the Secretary, Department of Health and Human ServicesInventors: Richard D. Beger, Jon G. Wilkes
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Publication number: 20110085971Abstract: The present invention provides a novel approach to cancer therapy and diagnostics that utilizes nanotubes and other similar nanostructures as both an indirect source of radiation therapy (BNCT), and as delivery vehicles for other types of radio- and chemo-therapeutic materials, as well as imaging agents for diagnostic purposes.Type: ApplicationFiled: October 15, 2010Publication date: April 14, 2011Inventors: Dan A. Buzatu, Jon G. Wilkes, Dwight Miller, Jerry A. Darsey, Tom Heinze, Alex Birls, Richard Beger
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Publication number: 20110085972Abstract: The present invention provides a novel approach to cancer therapy and diagnostics that utilizes nanotubes and other similar nanostructures as both an indirect source of radiation therapy (BNCT), and as delivery vehicles for other types of radio- and chemo-therapeutic materials, as well as imaging agents for diagnostic purposes.Type: ApplicationFiled: October 15, 2010Publication date: April 14, 2011Inventors: Dan A. Buzatu, Jon G. Wilkes, Dwight Miller, Jerry A. Darsey, Tom Heinze, Alex Birts, Richard Beger
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Publication number: 20110027174Abstract: The present invention provides a novel approach to cancer therapy and diagnostics that utilizes nanotubes and other similar nanostructures as both an indirect source of radiation therapy (BNCT), and as delivery vehicles for other types of radio- and chemo-therapeutic materials, as well as imaging agents for diagnostic purposes.Type: ApplicationFiled: October 15, 2010Publication date: February 3, 2011Inventors: Dan A. Buzatu, Jon G. Wilkes, Dwight Miller, Jerry A. Darsey, Tom Heinze, Alex Birls, Richard Beger
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Patent number: 7824660Abstract: The present invention provides a novel approach to cancer therapy and diagnostics that utilizes nanotubes and other similar nanostructures as both an indirect source of radiation therapy (BNCT), and as delivery vehicles for other types of radio- and chemo-therapeutic materials, as well as imaging agents for diagnostic purposes.Type: GrantFiled: April 30, 2008Date of Patent: November 2, 2010Inventors: Dan A. Buzatu, Jon G. Wilkes, Dwight Miller, Jerry A. Darsey, Tom Heinze, Alex Birls, Richard Beger
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Patent number: 7684934Abstract: A method for reproducibly analyzing mass spectra from different sample sources is provided. The method deconvolutes the complex spectra by collapsing multiple peaks of different molecular mass that originate from the same molecular fragment into a single peak. The differences in molecular mass are apparent differences caused by different charge states of the fragment and/or different metal ion adducts and/or reactant products of one or more of the charge states. The deconvoluted spectrum is compared to a library of mass spectra acquired from samples of known identity to unambiguously determine the identity of one or more components of the sample undergoing analysis.Type: GrantFiled: June 7, 2004Date of Patent: March 23, 2010Assignee: The United States of America as represented by the Department of Health and Human ServicesInventors: Alexandre Shvartsburg, Jon G. Wilkes, Paul Chiarelli, Ricky D. Holland, Dan A. Buzatu, Michael A. Beaudoin
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Patent number: 7608240Abstract: The present invention provides a novel approach to cancer therapy and diagnostics that utilizes nanotubes and other similar nanostructures as both an indirect source of radiation therapy (BNCT), and as delivery vehicles for other types of radio- and chemo-therapeutic materials, as well as imaging agents for diagnostic purposes.Type: GrantFiled: December 6, 2004Date of Patent: October 27, 2009Assignee: Board of Trustees of the University of ArkansasInventors: Dan A. Buzatu, Jon G. Wilkes, Dwight Miller, Jerry A. Darsey, Tom Heinze, Alex Biris, Richard Berger, Mark Diggs
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Publication number: 20090022655Abstract: The present invention provides a novel approach to cancer therapy and diagnostics that utilizes nanotubes and other similar nanostructures as both an indirect source of radiation therapy (BNCT), and as delivery vehicles for other types of radio- and chemo-therapeutic materials, as well as imaging agents for diagnostic purposes.Type: ApplicationFiled: April 30, 2008Publication date: January 22, 2009Inventors: Dan A. Buzatu, Jon G. Wilkes, Dwight Miller, Jerry A. Darsey, Tom Heinze, Alex Birls, Richard Beger
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Patent number: 7014816Abstract: A food quality indicator device an indicator compound provided on a substrate. The indicator compound changes color due to the presence of volatile compounds, such as volatile bases, in spoiled food, even when the food is frozen. Alternatively, the indicator compound detects the presence of an unwanted amine-producing biological agent, such as bacteria or fungi. The indicator compound is typically contained within a polymeric matrix disposed on the substrate. Examples of suitable indicator compounds include halogenated azo dyes, sulfonated xanthene dyes, and sulfonated hydroxy-functional triphenylmethane dyes.Type: GrantFiled: December 4, 2001Date of Patent: March 21, 2006Assignee: The United States of America as represented by the Department of Health and Human ServicesInventors: Dwight W. Miller, Jon G. Wilkes, Eric D. Conte
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Patent number: 6996472Abstract: Methods of compensating for drift in fingerprint spectra of microorganisms caused by changes in their environment are disclosed. These methods of compensating for drift permit identification of microorganisms from their fingerprint spectra regardless of the environment from which the microorganisms are obtained. Furthermore, the disclosed methods may be used to construct coherent databases of fingerprint spectra that may be expanded even though the standard database conditions are no longer experimentally achievable. In particular embodiments, methods of compensating for drift in pyrolysis mass spectra, constructing coherent pyrolysis mass spectral databases, and identifying bacteria from their pyrolysis mass spectra are disclosed.Type: GrantFiled: October 10, 2001Date of Patent: February 7, 2006Assignee: The United States of America as represented by the Department of Health and Human ServicesInventors: Jon G. Wilkes, Fatemeh Rafii, Katherine L. Glover, Manuel Holcomb, Xiaoxi Cao, John B. Sutherland
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Patent number: 6898533Abstract: Methods are disclosed for establishing a quantitative relationship between spectral properties of molecules and a biological, chemical, or physical endpoint of the molecules. Spectral data including data from nuclear magnetic resonance, mass spectrometric, infrared, and ultraviolet-visible techniques are used along with endpoint data to train a pattern-recognition program. The training yields a spectral data-activity relationship that may be used to predict the endpoint value of a molecule from its spectral data alone. Methods for rapidly screening isolated compounds or mixtures of compounds based upon their spectral data are included.Type: GrantFiled: July 31, 2000Date of Patent: May 24, 2005Assignee: The United States of America as represented by the Department of Health and Human ServicesInventors: Dwight W. Miller, Richard Beger, Jackson O. Lay, Jr., Jon G. Wilkes, James P. Freeman
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Publication number: 20040220749Abstract: Methods are disclosed for establishing a quantitative relationship between spectral properties of molecules and a biological, chemical, or physical endpoint of the molecules. Spectral data including data from nuclear magnetic resonance, mass spectrometric, infrared, and ultraviolet-visible techniques are used along with endpoint data to train a pattern-recognition program. The training yields a spectral data-activity relationship that may be used to predict the endpoint value of a molecule from its spectral data alone. Methods for rapidly screening isolated compounds or mixtures of compounds based upon their spectral data are included.Type: ApplicationFiled: June 8, 2004Publication date: November 4, 2004Applicant: The Govt. of the USA as represented by the Secretary of the Dept. of Health & Human ServicesInventors: Dwight W. Miller, Richard Beger, Jackson O. Lay, Jon G. Wilkes, James P. Freeman