Abstract: The breathing regulatory aid is a self-help tool, for people to become aware of their breathing and to train good breathing habits, with restful stops in the breathing cycle. The invention is a new easy to carry tool, that makes use of conscious thumb movements, to prompt and regulate the more unconscious breathing movements of the body. The device provides a platform for simple pendulum like movement of the thumb and the device has two elevated markers, one towards each end of the said pendulum movement. People can use the tool discreetly to help the mind stay focused on the task of breathing right. By using this device the awareness of the ebb and flow of the breathing cycle is increased. As a training tool it helps people to replace a short shallow breathing habit, with a fuller breathing pattern and include stop moments in the breathing cycle.

Abstract: Methods are provided for separating linear nucleic acid fragments based on their conformation. The methods are based on novel two-dimensional gel electrophoresis techniques. In one aspect the method comprises electrophoresing a sample of nucleic acids in a first dimension said sample through a gel matrix under a first set of pre-determined electrophoresis conditions; electrophoresing said gel matrix in a second dimension under a second set of electrophoresis conditions, such that linear nucleic acid fragments of equal length but having different conformation are separated; said first and second electrophoresis conditions are different, such that in one of said dimensions electrophoresis allows separation of linear nucleic acid fragments based on conformation and length, and in the other of said dimensions electrophoresis allows separation of the sample fragments based substantially on length.