Patents by Inventor Jon K. Magnuson
Jon K. Magnuson has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20240076700Abstract: Recombinant Aspergillus genetically modified to increase expression of g8846, renamed herein as aconitic acid exporter (aexA), are provided, which in some examples are also genetically inactivated for an endogenous cis-aconitic acid decarboxylase (cadA) gene. Such recombinant Aspergillus produce more aconitic acid as compared to native Aspergillus. Also provided are methods of using such recombinant Aspergillus to increase production of aconitic acid and other organic acids, such as citric acid, itaconic acid, and 3-hydroxypropionic acid (3-HP).Type: ApplicationFiled: October 24, 2023Publication date: March 7, 2024Applicant: Battelle Memorial InstituteInventors: Shuang Deng, Jon K. Magnuson, Joonhoon Kim, Kyle R. Pomraning, Ziyu Dai, Beth A. Hofstad
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Publication number: 20240052382Abstract: Provided herein are recombinant Aspergillus niger capable of producing 3-hydroxypropionic acid (3-HP). Also provided are methods of producing 3-hydroxypropionic acid (3-HP) and related kits.Type: ApplicationFiled: August 3, 2023Publication date: February 15, 2024Inventors: Kyle R. Pomraning, Ziyu Dai, Jon K. Magnuson, Beth A. Hofstad, Jeffrey J. Czajka, Joonhoon Kim, Shuang Deng
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Patent number: 11873523Abstract: Recombinant Aspergillus genetically modified to increase expression of g8846, renamed herein as aconitic acid exporter (aexA), are provided, which in some examples are also genetically inactivated for an endogenous cis-aconitic acid decarboxylase (cadA) gene. Such recombinant Aspergillus produce more aconitic acid as compared to native Aspergillus. Also provided are methods of using such recombinant Aspergillus to increase production of aconitic acid and other organic acids, such as citric acid, itaconic acid, and 3-hydroxypropionic acid (3-HP).Type: GrantFiled: June 14, 2021Date of Patent: January 16, 2024Assignee: Battelle Memorial InstituteInventors: Shuang Deng, Jon K. Magnuson, Joonhoon Kim, Kyle R. Pomraning, Ziyu Dai, Beth A. Hofstad
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Patent number: 11718854Abstract: Fungi that are genetically inactivated for the mstC gene (or a homolog thereof) are provided, which can also be genetically modified to increase production of heterologous proteins from a glucoamylase promoter. Methods of using these fungi, for example to degrade a biomass, are also provided.Type: GrantFiled: January 28, 2021Date of Patent: August 8, 2023Assignees: Battelle Memorial Institute, National Technology & Engineering Solutions of Sandia, LLCInventors: Scott E. Baker, Jon K. Magnuson, Morgann C. Reilly, Joonhoon Kim, John Gladden, Jed J. Lynn
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Patent number: 11414653Abstract: The present invention provides for an Aspergillus niger host cell comprising a gene of interest operatively linked to an ecm33 promoter of an ascomycete fungi, wherein the gene of interest is heterologous to the ecm33 promoter and/or to Aspergillus niger. In some embodiments, the gene of interest is a glycoside hydrolase enzyme. In some embodiments, the glycoside hydrolase enzyme is a glucosidase.Type: GrantFiled: September 6, 2018Date of Patent: August 16, 2022Assignees: National Technology & Engineering Solutions of Sandia, LLC, Battelle Memorial Institute, The Regents of the University of CaliforniaInventors: John M. Gladden, Saori Amaike Campen, Jinxiang Zhang, Jon K. Magnuson, Scott E. Baker, Blake A. Simmons
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Publication number: 20210388399Abstract: Recombinant Aspergillus genetically modified to increase expression of g8846, renamed herein as aconitic acid exporter (aexA), are provided, which in some examples are also genetically inactivated for an endogenous cis-aconitic acid decarboxylase (cadA) gene. Such recombinant Aspergillus produce more aconitic acid as compared to native Aspergillus. Also provided are methods of using such recombinant Aspergillus to increase production of aconitic acid and other organic acids, such as citric acid, itaconic acid, and 3-hydroxypropionic acid (3-HP).Type: ApplicationFiled: June 14, 2021Publication date: December 16, 2021Applicant: Battelle Memorial InstituteInventors: Shuang Deng, Jon K. Magnuson, Joonhoon Kim, Kyle R. Pomraning, Ziyu Dai, Beth A. Hofstad
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Publication number: 20210254077Abstract: Fungi that are genetically inactivated for the mstC gene (or a homolog thereof) are provided, which can also be genetically modified to increase production of heterologous proteins from a glucoamylase promoter. Methods of using these fungi, for example to degrade a biomass, are also provided.Type: ApplicationFiled: January 28, 2021Publication date: August 19, 2021Applicants: Battelle Memorial Institute, National Technology & Engineering Solutions of Sandia, LLCInventors: Scott E. Baker, Jon K. Magnuson, Morgann C. Reilly, Joonhoon Kim, John Gladden, Jed J. Lynn
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PRODUCTION OF ORGANIC ACIDS FROM ASPERGILLUS CIS-ACONITIC ACID DECARBOXYLASE (CADA) DELETION STRAINS
Publication number: 20210163966Abstract: This application provides recombinant Aspergillus fungi having an endogenous cis-aconitic acid decarboxylase (cadA) gene genetically inactivated, which allows aconitic acid production by the recombinant fungi. Such recombinant fungi can further include an exogenous nucleic acid molecule encoding aspartate decarboxylase (panD), an exogenous nucleic acid molecule encoding ?-alanine-pyruvate aminotransferase (BAPAT), and an exogenous nucleic acid molecule encoding 3-hydroxypropironate dehydrogenase (HPDH). Kits including these fungi, and methods of using these fungi to produce aconitic acid and 3-hydroxypropionic acid (3-HP) are also provided.Type: ApplicationFiled: February 10, 2021Publication date: June 3, 2021Applicant: Battelle Memorial InstituteInventors: Shuang Deng, Ziyu Dai, Jon K. Magnuson -
Patent number: 10947548Abstract: This application provides recombinant Aspergillus fungi having an endogenous cis-aconitic acid decarboxylase (cadA) gene genetically inactivated, which allows aconitic acid production by the recombinant fungi. Such recombinant fungi can further include an exogenous nucleic acid molecule encoding aspartate decarboxylase (panD), an exogenous nucleic acid molecule encoding ?-alanine-pyruvate aminotransferase (BAPAT), and an exogenous nucleic acid molecule encoding 3-hydroxypropionate dehydrogenase (HPDH). Kits including these fungi, and methods of using these fungi to produce aconitic acid and 3-hydroxypropionic acid (3-HP) are also provided.Type: GrantFiled: April 24, 2019Date of Patent: March 16, 2021Assignee: Battelle Memorial InstituteInventors: Shuang Deng, Ziyu Dai, Jon K. Magnuson
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Patent number: 10934551Abstract: Fungi that are genetically inactivated for the mstC gene (or a homolog thereof) are provided, which can also be genetically modified to increase production of heterologous proteins from a glucoamylase promoter. Methods of using these fungi, for example to degrade a biomass, are also provided.Type: GrantFiled: October 17, 2018Date of Patent: March 2, 2021Assignees: Battelle Memorial Institute, Natl Tech & Engineering Solutions of Sandia, LLCInventors: Scott E. Baker, Jon K. Magnuson, Morgann C. Reilly, Joonhoon Kim, John Gladden, Jed J. Lynn
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PRODUCTION OF ORGANIC ACIDS FROM ASPERGILLUS CIS-ACONITIC ACID DECARBOXYLASE (CADA) DELETION STRAINS
Publication number: 20190323020Abstract: This application provides recombinant Aspergillus fungi having an endogenous cis-aconitic acid decarboxylase (cadA) gene genetically inactivated, which allows aconitic acid production by the recombinant fungi. Such recombinant fungi can further include an exogenous nucleic acid molecule encoding aspartate decarboxylase (panD), an exogenous nucleic acid molecule encoding ?-alanine-pyruvate aminotransferase (BAPAT), and an exogenous nucleic acid molecule encoding 3-hydroxypropironate dehydrogenase (HPDH). Kits including these fungi, and methods of using these fungi to produce aconitic acid and 3-hydroxypropionic acid (3-HP) are also provided.Type: ApplicationFiled: April 24, 2019Publication date: October 24, 2019Applicant: Battelle Memorial InstituteInventors: Shuang Deng, Ziyu Dai, Jon K. Magnuson -
Publication number: 20190169584Abstract: The present invention provides for an Asperigillus niger host cell comprising a gene of interest operatively linked to an ecm33 promoter of an ascomycete fungi, wherein the gene of interest is heterologous to the ecm33 promoter and/or to Asperigillus niger. In some embodiments, the gene of interest is a glycoside hydrolase enzyme. In some embodiments, the glycoside hydrolase enzyme is a glucosidase.Type: ApplicationFiled: September 6, 2018Publication date: June 6, 2019Applicant: The Regents of the University of CaliforniaInventors: John M. Gladden, Saori Amaike Campen, Jinxiang Zhang, Jon K. Magnuson, Scott E. Baker, Blake A. Simmons
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Publication number: 20190112611Abstract: Fungi that are genetically inactivated for the mstC gene (or a homolog thereof) are provided, which can also be genetically modified to increase production of heterologous proteins from a glucoamylase promoter. Methods of using these fungi, for example to degrade a biomass, are also provided.Type: ApplicationFiled: October 17, 2018Publication date: April 18, 2019Inventors: Scott E. Baker, Jon K. Magnuson, Morgann C. Reilly, Joonhoon Kim, John Gladden, Jed J. Lynn
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Patent number: 9914932Abstract: This disclosure provides Agrobacterium-mediated transformation methods for the oil-producing (oleaginous) yeast Lipomyces sp., as well as yeast produced by the method. Such methods utilize Agrobacterium sp. cells that have a T-DNA binary plasmid, wherein the T-DNA binary plasmid comprises a first nucleic acid molecule encoding a first protein and a second nucleic acid molecule encoding a selective marker that permits growth of transformed Lipomyces sp. cells in selective culture media comprising an antibiotic.Type: GrantFiled: November 13, 2014Date of Patent: March 13, 2018Assignee: Battelle Memorial InstituteInventors: Ziyu Dai, Jon K. Magnuson, Shuang Deng, Kenneth S. Bruno, David E. Culley
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Publication number: 20160138031Abstract: This disclosure provides Agrobacterium-mediated transformation methods for the oil-producing (oleaginous) yeast Lipomyces sp., as well as yeast produced by the method. Such methods utilize Agrobacterium sp. cells that have a T-DNA binary plasmid, wherein the T-DNA binary plasmid comprises a first nucleic acid molecule encoding a first protein and a second nucleic acid molecule encoding a selective marker that permits growth of transformed Lipomyces sp. cells in selective culture media comprising an antibiotic.Type: ApplicationFiled: November 13, 2014Publication date: May 19, 2016Applicant: Battelle Memorial InstituteInventors: Ziyu Dai, Jon K. Magnuson, Shuang Deng, Kenneth S. Bruno, David E. Culley
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Patent number: 8735562Abstract: The present invention encompasses isolated gene regulatory elements and gene transcription terminators that are differentially expressed in a native fungus exhibiting a first morphology relative to the native fungus exhibiting a second morphology. The invention also encompasses a method of utilizing a fungus for protein or chemical production. A transformed fungus is produced by transforming a fungus with a recombinant polynucleotide molecule. The recombinant polynucleotide molecule contains an isolated polynucleotide sequence linked operably to another molecule comprising a coding region of a gene of interest. The gene regulatory element and gene transcription terminator may temporally and spatially regulate expression of particular genes for optimum production of compounds of interest in a transgenic fungus.Type: GrantFiled: October 23, 2008Date of Patent: May 27, 2014Assignee: Battelle Memorial InstituteInventors: Ziyu Dai, Linda L. Lasure, Jon K. Magnuson
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Publication number: 20140127754Abstract: Methods to convert lignocellulosic biomass to fermentable sugars with enzymes that degrade the lignocellulosic material are provided, as well as novel combinations of enzymes, including those that provide a synergistic release of sugars from plant biomass.Type: ApplicationFiled: November 5, 2012Publication date: May 8, 2014Applicant: DYADIC INTERNATIONAL, INC.Inventors: Marco A. Baez-Vasquez, Richard Burlingame, Jon K. Magnuson, Marion Bradford, Arkady Panteleimonovich Sinitsyn
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Patent number: 8304212Abstract: Methods to convert lignocellulosic biomass to fermentable sugars with enzymes that degrade the lignocellulosic material are provided, as well as novel combinations of enzymes, including those that provide a synergistic release of sugars from plant biomass.Type: GrantFiled: July 10, 2007Date of Patent: November 6, 2012Assignees: Dyadic International, Inc., Battelle Memorial Institute, Iowa Corn Promotion BoardInventors: Marco A. Baez-Vasquez, Richard Burlingame, Jon K. Magnuson, Marion Bradford, Arkady Panteleimonovich Sinitsyn
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Publication number: 20090068723Abstract: The present invention encompasses isolated gene regulatory elements and gene transcription terminators that are differentially expressed in a native fungus exhibiting a first morphology relative to the native fungus exhibiting a second morphology. The invention also encompasses a method of utilizing a fungus for protein or chemical production. A transformed fungus is produced by transforming a fungus with a recombinant polynucleotide molecule. The recombinant polynucleotide molecule contains an isolated polynucleotide sequence linked operably to another molecule comprising a coding region of a gene of interest. The gene regulatory element and gene transcription terminator may temporally and spatially regulate expression of particular genes for optimum production of compounds of interest in a transgenic fungus.Type: ApplicationFiled: October 23, 2008Publication date: March 12, 2009Applicant: BATTELLE MEMORIAL INSTITUTEInventors: Ziyu Dai, Linda L. Lasure, Jon K. Magnuson
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Patent number: 7449569Abstract: The present invention encompasses isolated gene regulatory elements and gene transcription terminators that are differentially expressed in a native fungus exhibiting a first morphology relative to the native fungus exhibiting a second morphology. The invention also encompasses a method of utilizing a fungus for protein or chemical production. A transformed fungus is produced by transforming a fungus with a recombinant polynucleotide molecule. The recombinant polynucleotide molecule contains an isolated polynucleotide sequence linked operably to another molecule comprising a coding region of a gene of interest. The gene regulatory element and gene transcription terminator may temporally and spatially regulate expression of particular genes for optimum production of compounds of interest in a transgenic fungus.Type: GrantFiled: August 17, 2004Date of Patent: November 11, 2008Assignee: Battelle Memorial InstituteInventors: Ziyu Dai, Linda L. Lasure, Jon K. Magnuson