Patents by Inventor Jon K. Magnuson

Jon K. Magnuson has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Patent number: 11414653
    Abstract: The present invention provides for an Aspergillus niger host cell comprising a gene of interest operatively linked to an ecm33 promoter of an ascomycete fungi, wherein the gene of interest is heterologous to the ecm33 promoter and/or to Aspergillus niger. In some embodiments, the gene of interest is a glycoside hydrolase enzyme. In some embodiments, the glycoside hydrolase enzyme is a glucosidase.
    Type: Grant
    Filed: September 6, 2018
    Date of Patent: August 16, 2022
    Assignees: National Technology & Engineering Solutions of Sandia, LLC, Battelle Memorial Institute, The Regents of the University of California
    Inventors: John M. Gladden, Saori Amaike Campen, Jinxiang Zhang, Jon K. Magnuson, Scott E. Baker, Blake A. Simmons
  • Publication number: 20210388399
    Abstract: Recombinant Aspergillus genetically modified to increase expression of g8846, renamed herein as aconitic acid exporter (aexA), are provided, which in some examples are also genetically inactivated for an endogenous cis-aconitic acid decarboxylase (cadA) gene. Such recombinant Aspergillus produce more aconitic acid as compared to native Aspergillus. Also provided are methods of using such recombinant Aspergillus to increase production of aconitic acid and other organic acids, such as citric acid, itaconic acid, and 3-hydroxypropionic acid (3-HP).
    Type: Application
    Filed: June 14, 2021
    Publication date: December 16, 2021
    Applicant: Battelle Memorial Institute
    Inventors: Shuang Deng, Jon K. Magnuson, Joonhoon Kim, Kyle R. Pomraning, Ziyu Dai, Beth A. Hofstad
  • Publication number: 20210254077
    Abstract: Fungi that are genetically inactivated for the mstC gene (or a homolog thereof) are provided, which can also be genetically modified to increase production of heterologous proteins from a glucoamylase promoter. Methods of using these fungi, for example to degrade a biomass, are also provided.
    Type: Application
    Filed: January 28, 2021
    Publication date: August 19, 2021
    Applicants: Battelle Memorial Institute, National Technology & Engineering Solutions of Sandia, LLC
    Inventors: Scott E. Baker, Jon K. Magnuson, Morgann C. Reilly, Joonhoon Kim, John Gladden, Jed J. Lynn
  • Publication number: 20210163966
    Abstract: This application provides recombinant Aspergillus fungi having an endogenous cis-aconitic acid decarboxylase (cadA) gene genetically inactivated, which allows aconitic acid production by the recombinant fungi. Such recombinant fungi can further include an exogenous nucleic acid molecule encoding aspartate decarboxylase (panD), an exogenous nucleic acid molecule encoding ?-alanine-pyruvate aminotransferase (BAPAT), and an exogenous nucleic acid molecule encoding 3-hydroxypropironate dehydrogenase (HPDH). Kits including these fungi, and methods of using these fungi to produce aconitic acid and 3-hydroxypropionic acid (3-HP) are also provided.
    Type: Application
    Filed: February 10, 2021
    Publication date: June 3, 2021
    Applicant: Battelle Memorial Institute
    Inventors: Shuang Deng, Ziyu Dai, Jon K. Magnuson
  • Patent number: 10947548
    Abstract: This application provides recombinant Aspergillus fungi having an endogenous cis-aconitic acid decarboxylase (cadA) gene genetically inactivated, which allows aconitic acid production by the recombinant fungi. Such recombinant fungi can further include an exogenous nucleic acid molecule encoding aspartate decarboxylase (panD), an exogenous nucleic acid molecule encoding ?-alanine-pyruvate aminotransferase (BAPAT), and an exogenous nucleic acid molecule encoding 3-hydroxypropionate dehydrogenase (HPDH). Kits including these fungi, and methods of using these fungi to produce aconitic acid and 3-hydroxypropionic acid (3-HP) are also provided.
    Type: Grant
    Filed: April 24, 2019
    Date of Patent: March 16, 2021
    Assignee: Battelle Memorial Institute
    Inventors: Shuang Deng, Ziyu Dai, Jon K. Magnuson
  • Patent number: 10934551
    Abstract: Fungi that are genetically inactivated for the mstC gene (or a homolog thereof) are provided, which can also be genetically modified to increase production of heterologous proteins from a glucoamylase promoter. Methods of using these fungi, for example to degrade a biomass, are also provided.
    Type: Grant
    Filed: October 17, 2018
    Date of Patent: March 2, 2021
    Assignees: Battelle Memorial Institute, Natl Tech & Engineering Solutions of Sandia, LLC
    Inventors: Scott E. Baker, Jon K. Magnuson, Morgann C. Reilly, Joonhoon Kim, John Gladden, Jed J. Lynn
  • Publication number: 20190323020
    Abstract: This application provides recombinant Aspergillus fungi having an endogenous cis-aconitic acid decarboxylase (cadA) gene genetically inactivated, which allows aconitic acid production by the recombinant fungi. Such recombinant fungi can further include an exogenous nucleic acid molecule encoding aspartate decarboxylase (panD), an exogenous nucleic acid molecule encoding ?-alanine-pyruvate aminotransferase (BAPAT), and an exogenous nucleic acid molecule encoding 3-hydroxypropironate dehydrogenase (HPDH). Kits including these fungi, and methods of using these fungi to produce aconitic acid and 3-hydroxypropionic acid (3-HP) are also provided.
    Type: Application
    Filed: April 24, 2019
    Publication date: October 24, 2019
    Applicant: Battelle Memorial Institute
    Inventors: Shuang Deng, Ziyu Dai, Jon K. Magnuson
  • Publication number: 20190169584
    Abstract: The present invention provides for an Asperigillus niger host cell comprising a gene of interest operatively linked to an ecm33 promoter of an ascomycete fungi, wherein the gene of interest is heterologous to the ecm33 promoter and/or to Asperigillus niger. In some embodiments, the gene of interest is a glycoside hydrolase enzyme. In some embodiments, the glycoside hydrolase enzyme is a glucosidase.
    Type: Application
    Filed: September 6, 2018
    Publication date: June 6, 2019
    Applicant: The Regents of the University of California
    Inventors: John M. Gladden, Saori Amaike Campen, Jinxiang Zhang, Jon K. Magnuson, Scott E. Baker, Blake A. Simmons
  • Publication number: 20190112611
    Abstract: Fungi that are genetically inactivated for the mstC gene (or a homolog thereof) are provided, which can also be genetically modified to increase production of heterologous proteins from a glucoamylase promoter. Methods of using these fungi, for example to degrade a biomass, are also provided.
    Type: Application
    Filed: October 17, 2018
    Publication date: April 18, 2019
    Inventors: Scott E. Baker, Jon K. Magnuson, Morgann C. Reilly, Joonhoon Kim, John Gladden, Jed J. Lynn
  • Patent number: 9914932
    Abstract: This disclosure provides Agrobacterium-mediated transformation methods for the oil-producing (oleaginous) yeast Lipomyces sp., as well as yeast produced by the method. Such methods utilize Agrobacterium sp. cells that have a T-DNA binary plasmid, wherein the T-DNA binary plasmid comprises a first nucleic acid molecule encoding a first protein and a second nucleic acid molecule encoding a selective marker that permits growth of transformed Lipomyces sp. cells in selective culture media comprising an antibiotic.
    Type: Grant
    Filed: November 13, 2014
    Date of Patent: March 13, 2018
    Assignee: Battelle Memorial Institute
    Inventors: Ziyu Dai, Jon K. Magnuson, Shuang Deng, Kenneth S. Bruno, David E. Culley
  • Publication number: 20160138031
    Abstract: This disclosure provides Agrobacterium-mediated transformation methods for the oil-producing (oleaginous) yeast Lipomyces sp., as well as yeast produced by the method. Such methods utilize Agrobacterium sp. cells that have a T-DNA binary plasmid, wherein the T-DNA binary plasmid comprises a first nucleic acid molecule encoding a first protein and a second nucleic acid molecule encoding a selective marker that permits growth of transformed Lipomyces sp. cells in selective culture media comprising an antibiotic.
    Type: Application
    Filed: November 13, 2014
    Publication date: May 19, 2016
    Applicant: Battelle Memorial Institute
    Inventors: Ziyu Dai, Jon K. Magnuson, Shuang Deng, Kenneth S. Bruno, David E. Culley
  • Patent number: 8735562
    Abstract: The present invention encompasses isolated gene regulatory elements and gene transcription terminators that are differentially expressed in a native fungus exhibiting a first morphology relative to the native fungus exhibiting a second morphology. The invention also encompasses a method of utilizing a fungus for protein or chemical production. A transformed fungus is produced by transforming a fungus with a recombinant polynucleotide molecule. The recombinant polynucleotide molecule contains an isolated polynucleotide sequence linked operably to another molecule comprising a coding region of a gene of interest. The gene regulatory element and gene transcription terminator may temporally and spatially regulate expression of particular genes for optimum production of compounds of interest in a transgenic fungus.
    Type: Grant
    Filed: October 23, 2008
    Date of Patent: May 27, 2014
    Assignee: Battelle Memorial Institute
    Inventors: Ziyu Dai, Linda L. Lasure, Jon K. Magnuson
  • Publication number: 20140127754
    Abstract: Methods to convert lignocellulosic biomass to fermentable sugars with enzymes that degrade the lignocellulosic material are provided, as well as novel combinations of enzymes, including those that provide a synergistic release of sugars from plant biomass.
    Type: Application
    Filed: November 5, 2012
    Publication date: May 8, 2014
    Applicant: DYADIC INTERNATIONAL, INC.
    Inventors: Marco A. Baez-Vasquez, Richard Burlingame, Jon K. Magnuson, Marion Bradford, Arkady Panteleimonovich Sinitsyn
  • Patent number: 8304212
    Abstract: Methods to convert lignocellulosic biomass to fermentable sugars with enzymes that degrade the lignocellulosic material are provided, as well as novel combinations of enzymes, including those that provide a synergistic release of sugars from plant biomass.
    Type: Grant
    Filed: July 10, 2007
    Date of Patent: November 6, 2012
    Assignees: Dyadic International, Inc., Battelle Memorial Institute, Iowa Corn Promotion Board
    Inventors: Marco A. Baez-Vasquez, Richard Burlingame, Jon K. Magnuson, Marion Bradford, Arkady Panteleimonovich Sinitsyn
  • Publication number: 20090068723
    Abstract: The present invention encompasses isolated gene regulatory elements and gene transcription terminators that are differentially expressed in a native fungus exhibiting a first morphology relative to the native fungus exhibiting a second morphology. The invention also encompasses a method of utilizing a fungus for protein or chemical production. A transformed fungus is produced by transforming a fungus with a recombinant polynucleotide molecule. The recombinant polynucleotide molecule contains an isolated polynucleotide sequence linked operably to another molecule comprising a coding region of a gene of interest. The gene regulatory element and gene transcription terminator may temporally and spatially regulate expression of particular genes for optimum production of compounds of interest in a transgenic fungus.
    Type: Application
    Filed: October 23, 2008
    Publication date: March 12, 2009
    Applicant: BATTELLE MEMORIAL INSTITUTE
    Inventors: Ziyu Dai, Linda L. Lasure, Jon K. Magnuson
  • Patent number: 7449569
    Abstract: The present invention encompasses isolated gene regulatory elements and gene transcription terminators that are differentially expressed in a native fungus exhibiting a first morphology relative to the native fungus exhibiting a second morphology. The invention also encompasses a method of utilizing a fungus for protein or chemical production. A transformed fungus is produced by transforming a fungus with a recombinant polynucleotide molecule. The recombinant polynucleotide molecule contains an isolated polynucleotide sequence linked operably to another molecule comprising a coding region of a gene of interest. The gene regulatory element and gene transcription terminator may temporally and spatially regulate expression of particular genes for optimum production of compounds of interest in a transgenic fungus.
    Type: Grant
    Filed: August 17, 2004
    Date of Patent: November 11, 2008
    Assignee: Battelle Memorial Institute
    Inventors: Ziyu Dai, Linda L. Lasure, Jon K. Magnuson