Patents by Inventor Jon Kemppainen
Jon Kemppainen has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 9464315Abstract: The invention relates to methods of separating polynucleotides, such as DNA, RNA and PNA, from solutions containing polynucleotides by reversibly binding the polynucleotides to a solid surface, such as a magnetic microparticle.Type: GrantFiled: March 25, 2013Date of Patent: October 11, 2016Assignee: Applied Biosystems, LLCInventors: Gary Latham, Xingwang Fang, Richard Conrad, Jon Kemppainen, Robert Setterquist, Brittan Pasloske
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Publication number: 20130302810Abstract: The invention relates to methods of separating polynucleotides, such as DNA, RNA and PNA, from solutions containing polynucleotides by reversibly binding the polynucleotides to a solid surface, such as a magnetic microparticle.Type: ApplicationFiled: March 25, 2013Publication date: November 14, 2013Inventors: Gary LATHAM, Xingwang Fang, Richard Conrad, Jon Kemppainen, Robert Setterquist, Brittan Pasloske
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Patent number: 8426126Abstract: The invention relates to methods of separating polynucleotides, such as DNA, RNA and PNA, from solutions containing polynucleotides by reversibly binding the polynucleotides to a solid surface, such as a magnetic microparticle.Type: GrantFiled: September 30, 2004Date of Patent: April 23, 2013Assignee: Applied Biosystems, LLCInventors: Gary J. Latham, Xingwang Fang, Richard C. Conrad, Jon A. Kemppainen, Robert A. Setterquist, Brittan L. Pasloske
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Publication number: 20110318811Abstract: Compositions and method for making and using a synthetic bovine DNase I are disclosed. More particularly, the sbDNase I of the present invention is a versatile enzyme that cleaves DNA nonspecifically to release 5?-phosphorylated nucleotides. The sbDNase I molecules of the present invention find particular use in a wide range of molecular biology applications, including: degradation of contaminating DNA after RNA isolation; RNA clean-up prior to, or in conjunction with, RT-PCR after in vitro transcription; identification of protein binding sequences on DNA (DNase I footprinting); prevention of clumping when handling cultured cells; tissue dissociation and creation of fragmented DNA for in vitro recombination reactions.Type: ApplicationFiled: May 9, 2011Publication date: December 29, 2011Applicant: APPLIED BIOSYSTEMS, LLCInventors: Gary Latham, Jon Kemppainen
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Patent number: 8020790Abstract: Improved ball mill disruption techniques. In different embodiments, disrupting particles that are not substantially spherical are used. In other embodiments, roughened disrupting particles are used. In other embodiments, larger disrupting particles are used. In each instance, improved disruption can be achieved.Type: GrantFiled: October 21, 2004Date of Patent: September 20, 2011Assignee: Applied Biosystems, LLCInventors: Jon Kemppainen, Gary J. Latham
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Publication number: 20080274458Abstract: The invention relates to a method of determining the amount of a target nucleic acid sequence in a sample, the method comprising: obtaining multiple distinguishable amplicons of the target nucleic acid sequence, each comprising a distinguishing tag and a target portion; amplifying the amplicons in a single reaction volume; and detecting nucleic acids amplified from the amplicons. Detection of the distinguishable amplicons can be varied in each of the steps of the method, which expands the dynamic range of the nucleic acid quantification methods and improves the reliability and accuracy of the methods.Type: ApplicationFiled: May 1, 2007Publication date: November 6, 2008Inventors: Gary J. Latham, Heidi J. Peltier, Jon Kemppainen, Timothy S. Davison, Emmanuel Labourier, David Brown, Matthew Winkler
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Publication number: 20080223962Abstract: Improved ball mill disruption techniques. In different embodiments, disrupting particles that are not substantially spherical are used. In other embodiments, roughened disrupting particles are used. In other embodiments, larger disrupting particles are used. In each instance, improved disruption can be achieved.Type: ApplicationFiled: October 21, 2004Publication date: September 18, 2008Inventors: Jon Kemppainen, Gary J. Latham
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Publication number: 20080131886Abstract: The present disclosure relates to methods of DNA amplification with a first primer that has a random sequence of nucleotides at its 3? end and a generic sequence 5? of the random nucleotides, as well as a second primer with the generic sequence of the first primer. The disclosure further relates to a method of precipitating DNA on a solid medium to improve DNA amplification. In a preferred embodiment, the presently disclosed methods are used for high-throughput genotyping of DNA samples, such as bloodstains or trace amounts of DNA.Type: ApplicationFiled: April 27, 2007Publication date: June 5, 2008Applicant: Invitrogen CorporationInventors: Wan Ji, Keqin Gregg, Bonnie Reus, Jon Kemppainen, Kristen Taylor, Scott Davis
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Publication number: 20080131878Abstract: Embodiments of the invention include methods of detecting one or more RNA by reverse transcribing one or more RNA target using one or more reverse transcription primer comprising in a 5? to 3? direction (i) a primer segment, (ii) a probe segment distinct from the primer segment, and (iii) a 3? target specific segment that anneals to a RNA target; amplifying one or more RNA from the reverse transcription reaction using a first amplification primer that anneals to the 3? end of a reverse transcribed RNA target and a second primer that anneals to a sequence complementary to the primer segment; and detecting amplification of a target nucleic acid.Type: ApplicationFiled: December 5, 2006Publication date: June 5, 2008Inventors: Gary J. Latham, Jon Kemppainen
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Publication number: 20060246503Abstract: The present disclosure relates to methods of DNA amplification with a first primer that has a random sequence of nucleotides at its 3? end and a generic sequence 5? of the random nucleotides, as well as a second primer with the generic sequence of the first primer. The disclosure further relates to a method of precipitating DNA on a solid medium to improve DNA amplification. In a preferred embodiment, the presently disclosed methods are used for high-throughput genotyping of DNA samples, such as bloodstains or trace amounts of DNA.Type: ApplicationFiled: July 10, 2006Publication date: November 2, 2006Inventors: Wan Ji, Keqin Gregg, Bonnie Reus, Jon Kemppainen, Kristen Taylor, Scott Davis
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Publication number: 20060228731Abstract: Compositions and method for making and using a synthetic bovine DNase I are disclosed. More particularly, the sbDNase I of the present invention is a versatile enzyme that cleaves DNA nonspecifically to release 5?-phosphorylated nucleotides. The sbDNase I molecules of the present invention find particular use in a wide range of molecular biology applications, including: degradation of contaminating DNA after RNA isolation; RNA clean-up prior to, or in conjunction with, RT-PCR after in vitro transcription; identification of protein binding sequences on DNA (DNase I footprinting); prevention of clumping when handling cultured cells; tissue dissociation and creation of fragmented DNA for in vitro recombination reactions.Type: ApplicationFiled: February 23, 2006Publication date: October 12, 2006Applicant: Ambion, Inc.Inventors: Gary Latham, Jon Kemppainen
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Patent number: 7067298Abstract: Compositions and method for making and using a synthetic bovine DNase I are disclosed. More particularly, the sbDNase I of the present invention is a versatile enzyme that cleaves DNA nonspecifically to release 5?-phosphorylated nucleotides. The sbDNase I molecules of the present invention find particular use in a wide range of molecular biology applications, including: degradation of contaminating DNA after RNA isolation; RNA clean-up prior to, or in conjunction with, RT-PCR after in vitro transcription; identification of protein binding sequences on DNA (DNase I footprinting); prevention of clumping when handling cultured cells; tissue dissociation and creation of fragmented DNA for in vitro recombination reactions.Type: GrantFiled: April 22, 2003Date of Patent: June 27, 2006Assignee: Ambion, Inc.Inventors: Gary Latham, Jon Kemppainen
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Publication number: 20050208510Abstract: The invention relates to methods of separating polynucleotides, such as DNA, RNA and PNA, from solutions containing polynucleotides by reversibly binding the polynucleotides to a solid surface, such as a magnetic microparticle.Type: ApplicationFiled: September 30, 2004Publication date: September 22, 2005Inventors: Gary Latham, Xingwang Fang, Richard Conrad, Jon Kemppainen, Robert Setterquist, Brittan Pasloske
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Publication number: 20050009015Abstract: A method and associated compositions for the relative quantification of nucleic acid on an address-defined surface, involving fitting the nucleic acid with a generic oligonucleotide, and hybridizing the generic oligonucleotide with a directly or indirectly labeled complementary oligonucleotide. The method is applicable, for example, to SNP genotyping and gene expression analysis.Type: ApplicationFiled: June 27, 2002Publication date: January 13, 2005Inventors: Wan Ji, Keqin Gregg, Bonnie Reus, Jon Kemppainen, Scott Davis
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Publication number: 20040219529Abstract: Compositions and method for making and using a synthetic bovine DNase I are disclosed. More particularly, the sbDNase I of the present invention is a versatile enzyme that cleaves DNA nonspecifically to release 5′-phosphorylated nucleotides. The sbDNase I molecules of the present invention find particular use in a wide range of molecular biology applications, including: degradation of contaminating DNA after RNA isolation; RNA clean-up prior to, or in conjunction with, RT-PCR after in vitro transcription; identification of protein binding sequences on DNA (DNase I footprinting); prevention of clumping when handling cultured cells; tissue dissociation and creation of fragmented DNA for in vitro recombination reactions.Type: ApplicationFiled: April 22, 2003Publication date: November 4, 2004Applicant: Ambion, Inc.Inventors: Gary Latham, Jon Kemppainen
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Publication number: 20040043416Abstract: The present disclosure relates to methods of DNA amplification with a first primer that has a random sequence of nucleotides at its 3′ end and a generic sequence 5′ of the random nucleotides, as well as a second primer with the generic sequence of the first primer. The disclosure further relates to a method of precipitating DNA on a solid medium to improve DNA amplification. In a preferred embodiment, the presently disclosed methods are used for high-throughput genotyping of DNA samples, such as bloodstains or trace amounts of DNA.Type: ApplicationFiled: September 26, 2003Publication date: March 4, 2004Applicant: Invitrogen CorporationInventors: Wan Ji, Keqin Gregg, Bonnie Reus, Jon Kemppainen, Kristen Taylor, Scott Davis
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Patent number: 6638722Abstract: The present disclosure relates to methods of DNA amplification with a first primer that has a random sequence of nucleotides at its 3′ end and a generic sequence 5′ of the random nucleotides, as well as a second primer with the generic sequence of the first primer. The disclosure further relates to a method of precipitating DNA on a solid medium to improve DNA amplification. In a preferred embodiment, the presently disclosed methods are used for high-throughput genotyping of DNA samples, such as bloodstains or trace amounts of DNA.Type: GrantFiled: June 13, 2001Date of Patent: October 28, 2003Assignee: Invitrogen CorporationInventors: Wan Ji, Keqin Gregg, Bonnie Reus, Jon Kemppainen, Kristen Taylor, Scott Davis
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Publication number: 20030108870Abstract: The present disclosure relates to methods of DNA amplification with a first primer that has a random sequence of nucleotides at its 3′ end and a generic sequence 5′ of the random nucleotides, as well as a second primer with the generic sequence of the first primer. The disclosure further relates to a method of precipitating DNA on a solid medium to improve DNA amplification. In a preferred embodiment, the presently disclosed methods are used for high-throughput genotyping of DNA samples, such as bloodstains or trace amounts of DNA.Type: ApplicationFiled: June 13, 2001Publication date: June 12, 2003Inventors: Wan Ji, Keqin Gregg, Bonnie Reus, Jon Kemppainen, Kristen Taylor, Scott Davis