Abstract: Provided are compositions and methods for using the compositions to modify eukaryotic chromosomes. The methods involve iteratively inserting DNA payloads into a chromosomal locus, or into multiple chromosomal loci. The methods utilize positive and negative selection approaches in combination with one or more recombinases to select cells that contain a payload, eliminate cells that do not contain a payload, and sequentially replace contiguous segments of the chromosome with subsequent payload insertions. Modified cells, and modified mammals containing modified cells, are included.

Abstract: Provided herein are methods and compositions for diagnosing a disease or an infection in a high throughput manner. Also provided herein are methods and compositions for diagnosing a disease or an infection with high specificity and high sensitivity.

Abstract: Provided herein are methods and compositions for diagnosing a disease or an infection in a high throughput manner. Also provided herein are methods and compositions for diagnosing a disease or an infection with high specificity and high sensitivity.

Abstract: The present disclosure provides methods for molecular neighborhood detection of molecules, such as by iterative proximity ligation or split-and-pool methods for obtaining positional information.

Abstract: An improved method and system for the determination of jitter buffers enables the generation of buffers having sizes and delays such that, as designed, the buffers capture a substantial majority of packets while not being resource intensive. The present methods and systems provide for improved jitter buffer management by deriving playout buffer adjustments from a plurality of variances, centered around a distribution peak, or mean average delay. The playout buffer monitor uses the buffer adjustments, in size and delay, to select, store and playout packets at their adjusted playout time. The present invention may be employed in a media gateway that enables data communications among heterogenous networks and may be specifically deployed to manage jitter experienced in the course of receiving packetized data and processing the data for further transmission through a packet-based or circuit-switched network.

Abstract: The present invention provides for adaptive filters that have improved computational and memory bandwidth proprieties. When applied to telecommunication applications, the present invention additionally provides for improved methods and systems of canceling echoes. In one embodiment of the adaptive filter of the present invention, a filter, preferably an adaptive finite impulse response (FIR) filter, of an appropriate length, N, is chosen. Once the filter is chosen, convergence is achieved and the filter is converted to an infinite impulse response (IIR) filter. In the course of operation, data is received from an input source and used to adapt the zeroes of the IIR filter using the least means square (LMS) approach, keeping the poles fixed. The adaptation process generates a set of converged filter coefficients that are then applied to the input signal to create a modified signal used to filter the data.

Abstract: An improved method and system for the determination of jitter buffers enables the generation of buffers having sizes and delays such that, as designed, the buffers capture a substantial majority of packets while not being resource intensive. The present methods and systems provide for improved jitter buffer management by deriving playout buffer adjustments from a plurality of variances, centered around a distribution peak, or mean average delay. The playout buffer monitor uses the buffer adjustments, in size and delay, to select, store and playout packets at their adjusted playout time. The present invention may be employed in a media gateway that enables data communications among heterogenous networks and may be specifically deployed to manage jitter experienced in the course of receiving packetized data and processing the data for further transmission through a packet-based or circuit-switched network.

Abstract: An improved method and system for the determination of jitter buffers enables the generation of buffers having sizes and delays such that, as designed, the buffers capture a substantial majority of packets while not being resource intensive. The present methods and systems provide for improved jitter buffer management by deriving playout buffer adjustments from a plurality of variances, centered around a distribution peak, or mean average delay. The playout buffer monitor uses the buffer adjustments, in size and delay, to select, store and playout packets at their adjusted playout time. The present invention may be employed in a media gateway that enables data communications among heterogenous networks and may be specifically deployed to manage jitter experienced in the course of receiving packetized data and processing the data for further transmission through a packet-based or circuit-switched network.

Abstract: The present invention provides for adaptive filters that have improved computational and memory bandwidth proprieties. When applied to telecommunication applications, the present invention additionally provides for improved methods and systems of canceling echoes. In one embodiment of the adaptive filter of the present invention, a filter, preferably an adaptive finite impulse response (FIR) filter, of an appropriate length, N, is chosen. Once the filter is chosen, convergence is achieved and the filter is converted to an infinite impulse response (IIR) filter. In the course of operation, data is received from an input source and used to adapt the zeroes of the IIR filter using the least means square (LMS) approach, keeping the poles fixed. The adaptation process generates a set of converged filter coefficients that are then applied to the input signal to create a modified signal used to filter the data.

Abstract: An improved method and system for the determination of jitter buffers enables the generation of buffers having sizes and delays such that, as designed, the buffers capture a substantial majority of packets while not being resource intensive. The present methods and systems provide for improved jitter buffer management by deriving playout buffer adjustments from a plurality of variances, centered around a distribution peak, or mean average delay. The playout buffer monitor uses the buffer adjustments, in size and delay, to select, store and playout packets at their adjusted playout time. The present invention may be employed in a media gateway that enables data communications among heterogenous networks and may be specifically deployed to manage jitter experienced in the course of receiving packetized data and processing the data for further transmission through a packet-based or circuit-switched network.

Abstract: The present invention provides for adaptive filters that have improved convergence, computational, and memory bandwidth proprieties. When applied to telecommunication applications, the present invention additionally provides for improved methods and systems of canceling echoes. In one embodiment of the adaptive filter of the present invention, a filter, preferably an adaptive finite impulse response (FIR) filter, of an appropriate length, N, is chosen. Once the filter is chosen, convergence is achieved using a convergence process. With convergence complete, the total energy of the filter coefficients is computed and two energy thresholds, a first energy threshold and a second energy threshold, are set relative to the computed total energy. Using the set energy thresholds, new filter coefficients are determined by relating the starting filter coefficient with the first energy threshold and the end filter coefficient with the second energy threshold.

Abstract: The present invention provides for adaptive filters that have improved computational and memory bandwidth proprieties. When applied to telecommunication applications, the present invention additionally provides for improved methods and systems of canceling echoes. In one embodiment of the adaptive filter of the present invention, a filter, preferably an adaptive finite impulse response (FIR) filter, of an appropriate length, N, is chosen. Once the filter is chosen, convergence is achieved and the filter is converted to an infinite impulse response (IIR) filter. In the course of operation, data is received from an input source and used to adapt the zeroes of the IIR filter using the least means square (LMS) approach, keeping the poles fixed. The adaptation process generates a set of converged filter coefficients that are then applied to the input signal to create a modified signal used to filter the data.