Abstract: A method for evaluating hepatocellular carcinoma in a subject is provided. In certain embodiments, the method comprises: a) obtaining a hepatocellular carcinoma protein marker profile for a sample obtained from the subject; and b) comparing the protein marker profile to a control profile.

Abstract: A flange assembly for a drum of an image forming apparatus. The flange assembly including a flange having a hollow portion that extends from a first end of the flange to a second end of the flange, and a grounding member provided within the hollow portion of the flange. The grounding member is configured to ground the drum by contacting a ground pin of the image forming apparatus when the drum and the flange assembly are mounted to the image forming apparatus. When the grounding member is provided within the hollow portion of the flange, an open space is provided along an entire length from the first end of the flange to the second end of the flange. The driving flange has a generally triangular-shaped drive member at a terminal end thereof, and the drive member is configured to mate with a drum drive assembly of the image forming apparatus.

Abstract: Pinch valves having a multi-piece valve body to receive flexible tubing are described herein. An example pinch valve includes a first body portion having a first recess and a second body portion having a second recess. The first body portion is movable relative to the second body portion. The first recess and the second recess define a passageway to receive a flexible tubing when the first body portion is coupled to the second body portion and the passageway is to retain the flexible tubing to prevent the flexible tubing from moving relative to the first and second body portions in an axial direction.

Abstract: Certain embodiments described in this disclosure relate to a method of sample analysis. In certain cases, the method comprises: a) contacting a genomic sample comprising double-stranded genomic DNA with a first restriction endonuclease that recognizes a nucleotide sequence that comprises a SNP site in the double stranded genomic DNA, wherein: i. the restriction endonuclease cleaves the genomic DNA at the sequence regardless of the allele of the SNP present at the SNP site; and ii. cleavage of the sequence by the restriction enzyme creates a 5? overhang that comprises the SNP site; b) contacting the digested genomic sample with a extension enzyme and a first labeled nucleotide that is used by the extension enzyme to fill in the overhang only if the overhang comprises a first allele of the SNP.

Abstract: Methods and systems are provided for using time-frequency warping to analyze a physiological signal. One embodiment includes applying a warping operator to the physiological signal based on the energy density of the signal. The warped physiological signal may be analyzed to determine whether non-physiological signal components are present. Further, the same warping operator may be applied to signal quality indicators, and the warped physiological signal may be analyzed based on the warped signal quality indicators. Non-physiological signal components, or types of non-physiological noise sources, may be identified based on a comparison of the physiological signal with the signal quality indicators. Non-physiological signal components may also be identified based on a neural network of known noise functions. In some embodiments, the non-physiological signal components may be removed to increase accuracy in estimating physiological parameters.

Abstract: A method of genome analysis is provided. In certain embodiments, the method comprises: a) contacting a double-stranded genomic DNA with a site-specific nicking endonuclease that recognizes a sequence comprising a single nucleotide polymorphism (SNP), in which the endonuclease nicks the genomic DNA at a nick site only if a first allele of the SNP is present; b) denaturing the genomic sample; c) contacting the denatured genomic sample with an array comprising a first probe and a second probe, in which nicking results in less binding of the denatured sample to the first probe relative to a sample that is not nicked; and d) comparing the amount of hybridization to the first probe to the amount of hybridization to said second probe, in which decreased binding of the denatured genomic samples to the first probe relative to the second probe indicates that the first allele of the SNP is present.

Abstract: A method of genome analysis is provided. In certain embodiments, the method of comprises: a) contacting a genomic sample comprising a double-stranded DNA with a site-specific nicking endonuclease to provide a nicked double-stranded DNA comprising a plurality of nick sites, in which the nicking endonuclease nicks a site adjacent to a variable nucleotide; b) contacting the nicked double-stranded DNA with a polymerase in the presence of a nucleotide composition comprising a first labeled nucleotide comprising a first label, thereby producing a labeled double-stranded DNA that is not labeled at every nick site; c) stretching out the labeled double-stranded DNA to provide a stretched, labeled double-stranded DNA; and d) imaging the stretched, labeled double-stranded DNA to identify a labeling pattern on the stretched labeled double-stranded DNA.

Abstract: A method of sample analysis is provided. In certain embodiments, the method comprises: a) site-specifically labeling a test genome with at least two different labels to produce a labeled genome labeled at a plurality of discrete sites across the genome; b) stretching a nucleic acid of the labeled genome to produce a linear pattern of the different labels along a region of a stretched nucleic acid; c) reading the labels along the region to provide a test pattern comprising a sequence of colors emitted by the labels; d) comparing the test pattern to a plurality of reference patterns obtained from a reference genome, in which the reference patterns are mapped to corresponding genomic locations in the reference genome; and e) identifying one or more reference patterns that match the test pattern, thereby mapping a location for the region in the test genome.

Abstract: A method for evaluating hepatocellular carcinoma in a subject is provided. In certain embodiments, the method comprises: a) obtaining a hepatocellular carcinoma protein marker profile for a sample obtained from the subject; and b) comparing the protein marker profile to a control profile.

Abstract: A flange assembly for a drum of an image forming apparatus. The flange assembly including a flange having a hollow portion that extends from a first end of the flange to a second end of the flange, and a grounding member provided within the hollow portion of the flange. The grounding member is configured to ground the drum by contacting a ground pin of the image forming apparatus when the drum and the flange assembly are mounted to the image forming apparatus. When the grounding member is provided within the hollow portion of the flange, an open space is provided along an entire length from the first end of the flange to the second end of the flange. The driving flange has a generally triangular-shaped drive member at a terminal end thereof, and the drive member is configured to mate with a drum drive assembly of the image forming apparatus.

Abstract: A reversible mounting bracket for mounting an article such as a loudspeaker is disclosed. The reversible mounting bracket in a first configuration is adapted to mount the article substantially flush to an upright mounting surface and in a second configuration, wherein the mounting bracket is reversed in orientation with respect to the article, the mounting bracket is adapted to mount the article to a mounting member.

Abstract: A reversible mounting bracket for mounting an article such as a loudspeaker is disclosed. The reversible mounting bracket in a first configuration is adapted to mount the article substantially flush to an upright mounting surface and in a second configuration, wherein the mounting bracket is reversed in orientation with respect to the article, the mounting bracket is adapted to mount the article to a mounting member.

Abstract: The invention generally relates to a complex mixture of oligonucleotide primers and/or probes. Another aspect of the invention includes a method of selective priming of a target nucleic acid.

Abstract: An active, patient-implementable, angular range-of-motion recovery practice, and associated structure, usable with respect to a knee including, from a methodologic point of view, (a) a occupying a sitting position, (b) by such occupying, defining a common swing plane for the upper and lower leg, (c) supporting the foot for back-and-forth, defined-swing-plane, rolling-contact motion over and in contact with an underlying motion-guidance surface, with such motion taking place generally within the defined swing plane, and (d) then swinging the lower leg in a fore-and-aft manner within the defined plane. The structure of the invention includes a rolling-motion traveler for supporting the underside of a patient's foot during practice of the invention, and a cooperative instruction set describing proper patient use of the traveler.

Abstract: Methods for enhancing the sensitivity of proximity ligation assays are provided herein. The methods make use of size separation methods, control of oligonucleotide size, and control of reaction conditions, to improve the assay sensitivity. Kits for performing the assay are also described.

Abstract: An artwork assembly and method is disclosed for providing a reveal between a dimensional support medium and a frame.

Abstract: A syrup delivery system delivers flavored syrup. A solenoid introduces air into the system and into an inlet of an exhaust diverter including a flexible diaphragm. The air exits the exhaust diverter through an outlet for entry into a syrup valve that dispenses the syrup. After dispensing of the syrup, the solenoid blocks air from entering the system. The air from the syrup valve reenters the exhaust diverter through the outlet. The air exits the exhaust diverter through the contaminated air exhaust. The air pushes on the flexible diaphragm, which contacts a seating surface around the inlet, preventing the air from exiting the exhaust diverter through the inlet. The remaining air in the system passes into the expansion tank and is diffused, causing any contaminates in the air to fall to the bottom of the expansion tank. The exhaust air is also subjected to a turbulent air flow path in the expansion tank that further separate any contaminants from the air.

Abstract: A syrup delivery system delivers flavored syrup. A solenoid introduces air into the system and into an inlet of an exhaust diverter including a flexible diaphragm. The air exits the exhaust diverter through an outlet for entry into a syrup valve that dispenses the syrup. After dispensing of the syrup, the solenoid blocks air from entering the system. The air from the syrup valve reenters the exhaust diverter through the outlet. The air exits the exhaust diverter through the contaminated air exhaust. The air pushes on the flexible diaphragm, which contacts a seating surface around the inlet, preventing the air from exiting the exhaust diverter through the inlet. The remaining air in the system passes into the expansion tank and is diffused, causing any contaminates in the air to fall to the bottom of the expansion tank. The exhaust air is also subjected to a turbulent air flow path in the expansion tank that further separate any contaminants from the air.

Abstract: A sweeping apparatus comprises a frame arranged for movement across a surface, a hitch on the frame arranged to be connected to a towing vehicle, drive wheels on the frame arranged to engage the surface and free wheels on the frame arranged to engage the surface. A brush arrangement coupled to the frame arranged to sweep up debris from the surface. A catch portion on the frame for receiving the debris. More than one gear assembly each arranged to be driven by atleast one of the drive wheels. A rotary brush member arranged to be rotated at a determined rate of speed by the gear assembly for engaging debris. A driven gear of the gear assembly coupled to and rotated by the drive wheel.