Abstract: The present invention has applications that include detecting color variation in a region, for example, color variations due to temperature changes in an area of ocean water, and, in a more specific application, detecting bioluminescence of certain organisms known to attach themselves to various objects. In one aspect of the invention, an acousto-optic tunable filter hyperspectral imaging system is moved across the region to collect a series of images in which each image represents the intensity of light at a different wavelength. In one embodiment, the acousto-optic tunable filter hyperspectral imaging system includes a motion platform for positioning the acousto-optic tunable filter hyperspectral imaging system over successive Y-coordinates of a region in a direction substantially parallel to a direction of motion of the motion platform.