Abstract: A method for differentiating areas in a series of digital images, the method comprising the steps of: providing a series of images comprising undetermined marker areas; evaluating every image 1n for 1?n?N according to predetermined selection criteria and defining image marker areas as undetermined marker areas fulfilling the predetermined selection criteria; providing a new image 1new; and inserting new image marker areas in the new image 1new, said new image marker areas having the same shape and location as image marker areas present in image 1n but not in image 1n?1, and said new image marker areas being identifiable in 1new by a unique feature. Further, the application discloses a method for visualizing cell populations in tissue sections of a histological sample. Further, the application discloses a method for visualizing three-dimensional distribution of multiple cell populations in a histological sample.

Abstract: The invention relates to a method of visualizing a plurality of cell types in a microscopic multicell tissue sample comprising cells of at least one particular cell type as well as cells of at least one confounding cell type, wherein cells of said at least one particular cell type and cells of said at least one confounding cell type both comprise a second cell marker and cells of the confounding cell type also comprise a first cell marker not present in cells of the at least one particular cell type, comprising the steps of exposing the cells of the sample to a first molecular detection means specifically binding to the first cell marker and having a detection enzyme; generating a blocking polymer within or around cells having the second cell marker by a reaction catalyzed by a detection enzyme on said first molecular detection means; exposing the cells of the sample to a second molecular detecting means comprising a fluorochrome, said second molecular detecting means specifically binding to said second cell

Abstract: A method for differentiating areas in a series of digital images, the method comprising the steps of: providing a series of images comprising undetermined marker areas; evaluating every image 1n for 1?n?N according to predetermined selection criteria and defining image marker areas as undetermined marker areas fulfilling the predetermined selection criteria; providing a new image 1new; and inserting new image marker areas in the new image 1new, said new image marker areas having the same shape and location as image marker areas present in image 1n but not in image 1n?1, and said new image marker areas being identifiable in 1new by a unique feature. Further, the application discloses a method for visualizing cell populations in tissue sections of a histological sample. Further, the application discloses a method for visualizing three-dimensional distribution of multiple cell populations in a histological sample.