Abstract: A filter comprising a flexible PCB with one or more signal tracks on one side. A electromagnetically absorbing material covers the signal tracks. An insulating material may be provided between the signal tracks and the electromagnetically absorbing material. The PCB is then folded or rolled to take up less space. The PCB may be first folded and then rolled to have both ends of the signal tracks available at the outer portion of the roll.

Abstract: A multi-channel filter with a PCB with a first side with signalling tracks and shielding tracks between neighbouring signalling tracks. On the second side, a conductive layer is provided. The signalling tracks are covered by an electromagnetically absorbing material, such as a powder of an electrically conducting material is provided. The filter may have sections with reversed structure where the conductors are on the second side and the layer on the first side, where the conductors on opposite sides are interconnected. The filter may be rolled or folded.

Abstract: A chip assembly for use in a microfluidic analysis system, such as a patch clamp apparatus, comprises a chip having an outer wall, a carrier structure comprising an aperture for receiving the chip, the aperture defining an inner wall, wherein the chip is arranged in the aperture with a liquid tight seal between the outer wall of the chip and the inner wall of the aperture. The chip may be sealed and bonded to the carrier structure by means of a bonding material, such as an UV curing adhesive. A through hole in the chip is aligned with the aperture in the carrier structure. A method for manufacturing the chip assembly is further disclosed.

Abstract: A handheld device for analysis of electrophysiological properties of a cellular membrane ion channel in an ion channel containing lipid cellular membrane includes a handheld body with a pump, an electronic controller, and a disposable pipette tip including a pathway for fluid. The pathway connects an open end of the pipette tip to an analysis substrate in the pipette tip. The substrate is adapted to transmit an electrical current through the ion channel. The handheld body and the disposable pipette tip are configured to releasably attach the pipette tip to the body, to provide a hydraulic connection between the pump and the pathway, and to provide an electric connection between the electronic controller and at least one of the electrodes of the substrate. The electronic controller of the handheld body is configured to operate the assembled device for microfluidic analysis of aspirated fluid.

Abstract: A chip assembly for use in a microfluidic analysis system, such as a patch clamp apparatus, comprises a chip having an outer wall, a carrier structure comprising an aperture for receiving the chip, the aperture defining an inner wall, wherein the chip is arranged in the aperture with a liquid tight seal between the outer wall of the chip and the inner wall of the aperture. The chip may be sealed and bonded to the carrier structure by means of a bonding material, such as an UV curing adhesive. A through hole in the chip is aligned with the aperture in the carrier structure. A method for manufacturing the chip assembly is further disclosed.

Abstract: A handheld device for analysis of electro-physiological properties of a cellular membrane ion channel in an ion channel containing lipid cellular membrane comprises a handheld body with a pump and an electronic controller, and a disposable pipette tip comprising a pathway for fluid, said pathway connecting an open end of the pipette tip to an analysis substrate comprised in the pipette tip. The substrate is adapted to transmit an electrical current through the ion channel in said ion channel-containing lipid cellular membrane, when said lipid cellular membrane is held at a predetermined site of the substrate, e.g. for patch clamp analysis. The handheld body and the disposable pipette tip are configured to releasably attach the pipette tip to the body, to provide a hydraulic connection between the pump of the handheld body and said pathway, and to provide an electric connection between the electronic controller and at least one of said electrodes of the substrate.

Abstract: A chip assembly for use in a microfluidic analysis system, such as a patch clamp apparatus, comprises a chip having an outer wall, a carrier structure comprising an aperture for receiving the chip, the aperture defining an inner wall, wherein the chip is arranged in the aperture with a liquid tight seal between the outer wall of the chip and the inner wall of the aperture. The chip may be sealed and bonded to the carrier structure by means of a bonding material, such as an UV curing adhesive. A through hole in the chip is aligned with the aperture in the carrier structure. A method for manufacturing the chip assembly is further disclosed.

Abstract: A microfluidics system comprising a channel having an inlet (32) and an outlet (38); a first membrane (31) positioned between the inlet (32) and outlet (38) and comprising an aperture having a radius within the range 0.1 to 50 ?m, the inlet (32) and the outlet (38) being in hydraulic communication with one another, such that a fluid can move along the channel from the inlet to the outlet.

Abstract: An electroosmotic flow pump for generating a flow in an electrolyte from an inlet to an outlet in a channel, the electroosmotic flow pump comprising a housing with the channel for holding the ionic solution, a membrane separating the channel in a first part in contact with the inlet and a second part in contact with the outlet, the membrane comprising a plurality of perforations having inner surface parts with a finite zeta potential in an 130-160 mM aqueous electrolyte with pH value in the interval 7-7.5, one or more first electrodes in electrical contact with electrolyte held in the first part of the channel and one or more second electrodes in electrical contact with electrolyte held in the second part of the channel, means for creating an electric potential difference between the first and second electrodes.

Abstract: The present invention relates to a substantially planar substrate for use in patch clamp analysis of the electrophysiological properties of a cell membrane comprising a glycocalyx, wherein the substrate comprises an aperture having a rim, the rim being adapted to form a gigaseal upon contact with the cell membrane, The invention further provides a method of making such a substrate and method for analysing the electrophysiological properties of a cell membrane comprising a glycocalyx.

Abstract: A microfluidics system comprising a channel having an inlet (32) and an outlet (38); a first membrane (31) positioned between the inlet (32) and outlet (38) and comprising an aperture having a radius within the range 0.1 to 50 ?m, the inlet (32) and the outlet (38) being in hydraulic communication with one another, such that a fluid can move along the channel from the inlet to the outlet.

Abstract: The present invention relates to screening methods, and in particular, to methods of screening DNA libraries to identify DNA molecules which when expressed in a host cell, give rise to at least one change in the phenotype of the host cell and such phenotype changes being detected using a patch clamp chip construct. Furthermore, the invention concerns the isolation of mRNA, corresponding to the DNA molecule, giving rise to said cellular phenotypic change.

Abstract: The present invention provides a pump for generating an Electroosmotic Flow (EOF) in a solution in a canal, guide, pipe or equivalent. Electroosmotic flow is generated by application of an electric field through a solution in a canal defined by insulating walls. The phenomenon depends on ionisation of sites on the surface so that for electroneutrality there is an excess mobile charge in the solution. The electric field acts on the excess charge in the solution causing the fluid to flow. The quantity and distribution of excess charge in the solution depends on the solution and the surface materials and is related to a parameter, the zeta (z) potential characterising material/solution combinations.

Abstract: The present invention relates to a substantially planar substrate for use in patch clamp analysis of the electrophysiological properties of a cell membrane comprising a glycocalyx, wherein the substrate comprises an aperture having a rim, the rim being adapted to form a gigaseal upon contact with the cell membrane. The invention further provides a method of making such a substrate and method for analysing the electrophysiological properties of a cell membrane comprising a glycocalyx.

Abstract: The present invention relates to a high through-put system for determining and/or monitoring electrophysiological properties of ion channels of ion channel-containing membranes, typically lipid membrane-containing structures such as cells. In particular, the invention provides a substrate which provides means for automatically positioning cells at measuring sites using electroosmotic flow in canals formed on or in the substrate. The electroosmotic flow is generated and controlled by electroosmotic flow pumps integrated on the substrate or positioned in relation thereto. Thereby, cells can be positioned in favorable measurement configuration at a plurality of sites for performing testing and measurements. Also, the invention relates to a main electric circuit for performing testing and measurements on a plurality of cells in parallel.

Abstract: The present invention provides a pump for generating an Electroosmotic Flow (EOF) in a solution in a canal, guide, pipe or equivalent. Electroosmotic flow is generated by application of an electric field through a solution in a canal defined by insulating walls. The phenomenon depends on ionisation of sites on the surface so that for electroneutrality there is an excess mobile charge in the solution. The electric field acts on the excess charge in the solution causing the fluid to flow. The quantity and distribution of excess charge in the solution depends on the solution and the surface materials and is related to a parameter, the zeta (z) potential characterising material/solution combinations.

Abstract: The present invention provides a pump for generating an Electroosmotic Flow (EOF) in a solution in a canal, guide, pipe or equivalent. Electroosmotic flow is generated by application of an electric field through a solution in a canal defined by insulating walls. The phenomenon depends on ionisation of sites on the surface so that for electroneutrality there is an excess mobile charge in the solution. The electric field acts on the excess charge in the solution causing the fluid to flow. The quantity and distribution of excess charge in the solution depends on the solution and the surface materials and is related to a parameter, the zeta (z) potential characterising material/solution combinations.

Abstract: The present invention retates to a substantially planar substrate for use in patch clamp analysis of the electrophysiological properties of a cell membrane comprising a glycocalyx, wherein the substrate comprises an aperture having a rim, the rim being adapted to form a gigaseal upon contact with the cell membrane, The invention further provides a method of making such a substrate and method for analysing the electrophysiological properties of a cell membrane comprising a glycocalyx.

Abstract: The present invention relates to a high through-put system for determining and/or monitoring electrophysiological properties of ion channels of ion channel-containing membranes, typically lipid membrane-containing structures such as cells. In particular, the invention provides a substrate which provides means for automatically positioning cells at measuring sites using electroosmotic flow in canals formed on or in the substrate. The electroosmotic flow is generated and controlled by electroosmotic flow pumps integrated on the substrate or positioned in relation thereto. Thereby, cells can be positioned in favorable measurement configuration at a plurality of sites for performing testing and measurements. Also, the invention relates to a main electric circuit for performing testing and measurements on a plurality of cells in parallel.