Abstract: The present invention relates to systems which harness the molecular biology of a living cell to direct evolution of biological entities of interest. According to the invention, a host cell is engineered to facilitate mutation of a nucleic acid target corresponding to that entity and select for desirable mutants. As applied to populations of host cells, the invention provides a means to generate and contemporaneously select mutants of interest, allowing for the production of extremely diverse libraries enriched in the most ‘fit’ mutants.

Abstract: A system and method for simulating the electrical operation of a mixed analog/digital system includes the capability for analog circuit block inputs to respond to the condition in which digital gate outputs connected to the analog circuit block input are presented in high-impedance or floating signal states, thereby providing for simulation of a wide variety of mixed analog/digital designs in which this condition occurs. In a simulated design, an analog input of one or more analog circuit blocks is transformed into an analog tri-statable input-output referred to as an ioput. The ioput is capable of driving an analog signal when the digital gate outputs connected to the analog block input are presented in a high-impedance Z state; otherwise, the ioput acts as an analog input to the analog circuit block.

Abstract: The present invention relates to systems which harness the molecular biology of a living cell to direct evolution of biological entities of interest. According to the invention, a host cell is engineered to facilitate mutation of a nucleic acid target corresponding to that entity and select for desirable mutants. As applied to populations of host cells, the invention provides a means to generate and contemporaneously select mutants of interest, allowing for the production of extremely diverse libraries enriched in the most ‘fit’ mutants.