Patents by Inventor Jonathan A. Zerkowski

Jonathan A. Zerkowski has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Production of dirhamnose-lipid in recombinant nonpathogenic bacterium
    Patent number: 10190144
    Abstract: Pseudomonas chlororaphis NRRL B-30761 produces monorhamnolipids with predominantly 3-hydroxydodecenoyl-3-hydroxydecanoate (C12:1-C10) or 3-hydroxydodecanoyl-3-hydroxydecanoate (C12-C10) as the lipid moiety under static growth conditions. The cloning and sequencing of three genes and proteins involved in the biosynthesis of monorhamnose-lipid (R1L) is described. Expression of two of these genes, i.e., rhlA and rhlB, together in P. chlororaphis NRRL B-30761 increases R1L production by at least 10-fold. Also the generation of a recombinant P. chlororaphis NRRL B-30761 capable of synthesizing dirhamnose-lipid (R2L) is described. Characterization of R1L and R2L produced by the recombinant P. chlororaphis NRRL B-30761 is also described.
    Type: Grant
    Filed: June 3, 2014
    Date of Patent: January 29, 2019
    Assignee: The United States of America, as represented by the Secretary of Agriculture
    Inventors: Daniel Solaiman, Richard D. Ashby, Jonathan A. Zerkowski, Nereus W. Gunther
  • Use of glucosyltransferase gene
    Patent number: 9388444
    Abstract: The isolation and characterization of a glucosyltransferase gene from Candida bombicola is disclosed. Use of the glucosyltransferase enzyme for the production of glucosylated sterol and hydroxyl-fatty acid substrates is also disclosed. This enzyme has broad-specificity and is useful for the production of sophorolipids both in-vivo and in-vitro.
    Type: Grant
    Filed: April 24, 2015
    Date of Patent: July 12, 2016
    Assignee: The United States of America, as represented by the Secretary of Agriculture
    Inventors: Daniel K Solaiman, Richard D Ashby, Jonathan A. Zerkowski, Robert A Moreau, Yanhong Liu
  • PRODUCTION OF DIRHAMNOSE-LIPID IN RECOMBINANT NONPATHOGENIC BACTERIUM PSEUDOMONAS CHLORORAPHIS
    Publication number: 20160102330
    Abstract: Pseudomonas chlororaphis NRRL B-30761 produces monorhamnolipids with predominantly 3-hydroxydodecenoyl-3-hydroxydecanoate (C12:1-C10) or 3-hydroxydodecanoyl-3-hydroxydecanoate (C12-C10) as the lipid moiety under static growth conditions. The cloning and sequencing of three genes and proteins involved in the biosynthesis of monorhamnose-lipid (R1L) is described. Expression of two of these genes, i.e., rhlA and rhlB, together in P. chlororaphis NRRL B-30761 increases R1L production by at least 10-fold. Also the generation of a recombinant P. chlororaphis NRRL B-30761 capable of synthesizing dirhamnose-lipid (R2L) is described. Characterization of R1L and R2L produced by the recombinant P. chlororaphis NRRL B-30761 is also described.
    Type: Application
    Filed: June 3, 2014
    Publication date: April 14, 2016
    Inventors: Daniel Solaiman, Richard D. ASHBY, Jonathan A. ZERKOWSKI, Nereus W. GUNTHER
  • NOVEL USE OF GLUCOSYLTRANSFERASE GENE
    Publication number: 20150267236
    Abstract: The isolation and characterization of a glucosyltransferase gene from Candida bombicola is disclosed. Use of the glucosyltransferase enzyme for the production of glucosylated sterol and hydroxyl-fatty acid substrates is also disclosed. This enzyme has broad-specificity and is useful for the production of sophorolipids both in-vivo and in-vitro.
    Type: Application
    Filed: April 24, 2015
    Publication date: September 24, 2015
    Inventors: Daniel K Solaiman, Richard D Ashby, Jonathan A. Zerkowski, Robert A Moreau, Yanhong Liu