Patents by Inventor Jonathan Abysalh

Jonathan Abysalh has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Methods For Purification of Messenger RNA
    Publication number: 20210180041
    Abstract: The present invention relates, in part, to methods for large-scale purification of mRNA. The method includes, at least, a step of centrifuging an mRNA suspension in a centrifuge comprising a porous substrate at a speed sufficient to remove process contaminants and to precipitate purified mRNA composition onto the porous substrate.
    Type: Application
    Filed: December 22, 2020
    Publication date: June 17, 2021
    Inventors: Frank DeRosa, Michael Heartlein, Jonathan Abysalh, Daniel Crawford, Anusha Dias, Shrirang Karve
  • Methods For Purification of Messenger RNA
    Publication number: 20210123041
    Abstract: The present invention relates, in part, to methods for large-scale purification of mRNA. The method includes, at least, steps of forming an mRNA slurry, stirring the slurry, and vacuum or pressure filtering the slurry.
    Type: Application
    Filed: October 14, 2020
    Publication date: April 29, 2021
    Inventors: Jonathan Abysalh, Daniel Crawford, Frank DeRosa, Shrirang Karve, Anusha Dias, Michael Heartlein
  • Methods for purification of messenger RNA
    Patent number: 10975369
    Abstract: The present invention relates, in part, to methods for large-scale purification of mRNA. The method includes, at least, a step of centrifuging an mRNA suspension in a centrifuge comprising a porous substrate at a speed sufficient to remove process contaminants and to precipitate purified mRNA composition onto the porous substrate.
    Type: Grant
    Filed: February 27, 2018
    Date of Patent: April 13, 2021
    Assignee: Translate Bio, Inc.
    Inventors: Frank DeRosa, Michael Heartlein, Jonathan Abysalh, Daniel Crawford, Anusha Dias, Shrirang Karve
  • METHODS FOR PURIFICATION OF MESSENGER RNA
    Publication number: 20210002635
    Abstract: The present invention provides, among other things, methods for purifying high quality messenger RNA (mRNA) suitable for clinical use, without using any caustic or flammable solvents. The present invention is, in part, based on surprising discovery that mRNA can be successfully purified by selective precipitation and washing without using ethanol while maintaining integrity and high purity of mRNA. Thus, the present invention provides an effective, reliable, and safer method of purifying RNA from large scale manufacturing process therapeutic applications without using any caustic or inflammable solvents.
    Type: Application
    Filed: May 15, 2020
    Publication date: January 7, 2021
    Inventors: Jonathan Abysalh, Frank DeRosa, Jorel Vargas, Cameron M. Smith
  • Methods for purification of messenger RNA
    Patent number: 10808241
    Abstract: The present invention relates, in part, to methods for large-scale purification of mRNA. The method includes, at least, steps of forming an mRNA slurry, stirring the slurry, and vacuum or pressure filtering the slurry.
    Type: Grant
    Filed: February 27, 2018
    Date of Patent: October 20, 2020
    Assignee: Translate Bio, Inc.
    Inventors: Jonathan Abysalh, Daniel Crawford, Frank DeRosa, Shrirang Karve, Anusha Dias, Michael Heartlein
  • METHODS FOR PURIFICATION OF MESSENGER RNA
    Publication number: 20200095571
    Abstract: The present invention provides, among other things, methods for purifying mRNA based on normal flow filtration for therapeutic use.
    Type: Application
    Filed: August 26, 2019
    Publication date: March 26, 2020
    Inventors: Joseph Parella, Kimberly Gillis, Jonathan Abysalh, Travis Jeannotte, Erik Held, Frank DeRosa, Michael Heartlein, Rebecca Powell
  • TREATMENT OF CYSTIC FIBROSIS BY DELIVERY OF CODON-OPTIMIZED mRNA ENCODING CFTR
    Publication number: 20180333457
    Abstract: The present invention provides, among other things, methods of treating cystic fibrosis, comprising a step of administering to a subject in need of treatment a composition comprising an mRNA encoding a Cystic Fibrosis Transmembrane Conductance Regulator (CFTR) protein, wherein the mRNA encoding the CFTR protein comprises a polynucleotide sequence at least 80% identical to SEQ ID NO: 1, wherein the mRNA is at a concentration of at least 0.4 mg/mL, and wherein the step of administering comprises inhalation.
    Type: Application
    Filed: May 16, 2018
    Publication date: November 22, 2018
    Inventors: Michael Heartlein, Frank DeRosa, Alan Kimura, Jonathan Abysalh, Anusha Dias, Shrirang Karve, Zarna Patel
  • Novel Codon-Optimized CFTR MRNA
    Publication number: 20180256741
    Abstract: The present invention provides, among other things, improved methods and pharmaceutical compositions for treating cystic fibrosis based on codon optimized mRNA encoding a Cystic Fibrosis Transmembrane Conductance Regulator (CFTR) protein.
    Type: Application
    Filed: February 27, 2018
    Publication date: September 13, 2018
    Inventors: Anusha Dias, Jonathan Abysalh, Brian Bettencourt, Frank DeRosa, Michael Heartlein
  • LARGE SCALE SYNTHESIS OF MESSENGER RNA
    Publication number: 20180258423
    Abstract: The present invention provides methods for large-scale production of a composition enriched for full-length mRNA molecules using an SP6 RNA polymerase and compositions produced using such methods and uses thereof.
    Type: Application
    Filed: February 27, 2018
    Publication date: September 13, 2018
    Inventors: Anusha Dias, Daniel Crawford, Frank DeRosa, Jonathan Abysalh, Michael Heartlein
  • METHODS FOR PURIFICATION OF MESSENGER RNA
    Publication number: 20180251754
    Abstract: The present invention relates, in part, to methods for large-scale purification of mRNA. The method includes, at least, a step of centrifuging an mRNA suspension in a centrifuge comprising a porous substrate at a speed sufficient to remove process contaminants and to precipitate purified mRNA composition onto the porous substrate.
    Type: Application
    Filed: February 27, 2018
    Publication date: September 6, 2018
    Inventors: Frank DeRosa, Michael Heartlein, Jonathan Abysalh, Daniel Crawford, Anusha Dias, Shrirang Karve
  • Methods For Purification of Messenger RNA
    Publication number: 20180251755
    Abstract: The present invention relates, in part, to methods for large-scale purification of mRNA. The method includes, at least, steps of forming an mRNA slurry, stirring the slurry, and vacuum or pressure filtering the slurry.
    Type: Application
    Filed: February 27, 2018
    Publication date: September 6, 2018
    Inventors: Jonathan Abysalh, Daniel Crawford, Frank DeRosa, Shrirang Karve, Anusha Dias, Michael Heartlein