Abstract: CRISPR-based gene-drive system for inhibiting antibiotic resistance of bacteria, including Escherichia coli that efficiently copies a gRNA cassette and adjacent cargo that are flanked with sequences homologous to the targeted gRNA/Cas9 cleavage site. This “pro-active” genetic system (Pro-AG) functionally inactivates an antibiotic resistance marker on a high copy number plasmid with greater efficiency than control CRISPR-based methods. Pro-AG can effectively edit large plasmids or single-copy genomic targets, or introduce functional genes, with numerous applications to biotechnology and biomedicine.

Abstract: This method includes emitting, by an emitter at an emitting depth, moved along an axis, at least one incident sound wave at an emitting frequency, receiving a first sound wave reflected by a first reflective object at a first depth and a second sound wave reflected by a second reflective object at a second depth, greater than the first depth, providing a first velocity at the first depth, and determining a second velocity of the sound waves at the second depth, from the frequencies of the first and second reflected sound waves, the emitting frequency and the first velocity.

Abstract: This method includes emitting, by an emitter at an emitting depth, moved along an axis, at least one incident sound wave at an emitting frequency, receiving a first sound wave reflected by a first reflective object at a first depth and a second sound wave reflected by a second reflective object at a second depth, greater than the first depth, providing a first velocity at the first depth, and determining a second velocity of the sound waves at the second depth, from the frequencies of the first and second reflected sound waves, the emitting frequency and the first velocity.

Abstract: A pharmaceutical formulation is described comprising a therapeutically effective amount of an antibody, an acetate buffer, a lyoprotectant, a bulking agent and a surfactant.

Abstract: A pharmaceutical formulation is described comprising a therapeutically effective amount of an antibody, an acetate buffer, a lyoprotectant, a bulking agent and a surfactant.