Abstract: A microfluidic apparatus and methods thereof. The Apparatus having a flat and thin substrate, the substrate including at least one microfluidic testing device, each device with: plurality of Stationary Nanoliter Droplet Array (SNDA) components; a common inlet port and a distribution manifold, configured to enable an introduction of a fluid into all the primary channels; plurality of individual inlet ports, each coupled to a different primary channel, configured to enable an individual introduction of a fluid into its associated primary channel; and one or more outlet ports and optionally a collecting manifold, configured to evacuate liquid and/or gas flowing out thereof.

Abstract: Microfluidic devices and methods thereof; the devices including: SNDA components; each SNDA component comprising: a primary channel; secondary channels; and nano-wells that are each open to the primary channel and are each connected via vents to the secondary channel; the vents are configured to enable passage of gas solely from the nano-wells to the secondary channel, such that when a fluid is introduced into the primary channel it fills the nano-wells, and the originally accommodated gas is evacuated via the vents and the secondary channel/s; a common inlet port, configured to enable a simultaneous introduction of the fluid into all the primary channels of the different SNDA components; individual inlet ports, configured to enable individual introduction of fluid, each into a different primary channel of a different SNDA component; and at least one outlet port, configured to enable evacuation of the gas out of all the secondary channels.

Abstract: A device comprising: plurality of Stationary Nanoliter Droplet Array (SNDA) components; each SNDA component comprising: at least one primary channel; at least one secondary channel; and a plurality of nano-wells that are each open to the primary channel and are each connected by one or more vents to the secondary channel; the vents are configured to enable passage of air solely from the nano-wells to the secondary channel, such that when a liquid is introduced into the primary channel it fills the nano-wells, and the originally accommodated air is evacuated via the vents and the secondary channel/s; an inlet port and a distribution channel configured to enable a simultaneous introduction of the liquid into all primary channels; and an outlet port and an evacuation channel configured to enable a simultaneous evacuation of the air out of all the secondary channels.

Abstract: A microfluidic device may include a microstructure formed in a substrate, the microstructure including a primary channel with a first end and a second end, and a plurality of chambers that open to the primary channel. At least two openings coupled to the first end of the primary channel may be used to load at least two fluid streams into the device through the first end of the primary channel to flow along the primary channel from the first end to the second end into the plurality of chambers, each chamber of the plurality of chambers having a volume less than 100 nanoliters and connected by a vent to a secondary channel in the micro structure, a width of the vent configured to enable a gas to escape from the chamber to the secondary channel while inhibiting the flow of said at least first and second fluid streams into the secondary channel.