Abstract: The present disclosure provides systems and methods for providing training opportunities based on data collected from monitoring a physiological parameter of persons engaged in physical activity. The physical activity can be a sporting activity, such as a contact sport (e.g., football, hockey, lacrosse) or a recreational activity or sport (e.g., biking, hiking, skiing, snowboarding, motorsports). The system is configured with select components that perform a method of (i) recording data related to a physiological parameter of a person engaged in a physical activity (e.g., an impact received by a player engaged in a contact sport), (ii) analyzing the recorded data related to the physiological parameter while the person is engaged in a physical activity (e.g., is the received impact greater than a predetermined threshold), and (iii) providing post-physical activity analysis of the recorded data to make suggested changes in how the person engages in the physical activity.

Abstract: A measuring tape device may include a housing having an aperture, a reel assembly defining an axis, a blade having a first end configured to extend from the housing through the aperture and a second end configured to be wound on the reel assembly, and a transverse aperture extending through the housing in a direction substantially parallel to the axis.

Abstract: A measuring tape device may include a housing having an aperture, a reel assembly defining an axis, a blade having a first end configured to extend from the housing through the aperture and a second end configured to be wound on the reel assembly, and a transverse aperture extending through the housing in a direction substantially parallel to the axis.

Abstract: A measuring tape device may include a housing having an aperture, a reel assembly defining an axis, a blade having a first end configured to extend from the housing through the aperture and a second end configured to be wound on the reel assembly, a locking assembly configured to alternately allow and prevent winding of the blade onto the reel assembly based on a position of the locking assembly, and a guard assembly comprising guard members extending on opposing lateral sides of the locking assembly to protect the locking assembly from impact.

Abstract: A measuring tape device may include a housing having an aperture, a reel assembly defining an axis, a blade having a first end configured to extend from the housing through the aperture and a second end configured to be wound on the reel assembly, and a transverse aperture extending through the housing in a direction substantially parallel to the axis.

Abstract: A spool for a chalk box may include a core member extending substantially parallel to an axis of rotation of the spool, a first sidewall disposed in a plane substantially perpendicular to the axis of rotation of the spool at a first end of the core member, and a second sidewall disposed in a plane substantially parallel to the first sidewall at a second end of the core member. The string may be configured to be retained on the spool by being wound onto the core member. The first sidewall may include a first anchorage orifice and a second anchorage orifice spaced apart from each other and each passing through the first sidewall from an inside surface of the first sidewall to an outside surface of the first sidewall. A spool groove may be formed on the outside surface of the first sidewall extending from the first anchorage orifice to the second anchorage orifice.

Abstract: Provided herein are host cells or host cellular expression systems that express a membrane protein. Also, methods are provided that use such host cells or host cellular expression systems to produce higher amounts of the membrane proteins. Further, the cells or cellular systems can be used as tools for the functional characterization of membrane proteins, as well as for screening and drug discovery efforts targeting membrane proteins.

Abstract: The present disclosure provides systems and methods for providing training opportunities based on data collected from monitoring a physiological parameter of persons engaged in physical activity. The physical activity can be a sporting activity, such as a contact sport (e.g., football, hockey, lacrosse) or a recreational activity or sport (e.g., biking, hiking, skiing, snowboarding, motorsports). The system is configured with select components that perform a method of (i) recording data related to a physiological parameter of a person engaged in a physical activity (e.g., an impact received by a player engaged in a contact sport), (ii) analyzing the recorded data related to the physiological parameter while the person is engaged in a physical activity (e.g., is the received impact greater than a predetermined threshold), and (iii) providing post-physical activity analysis of the recorded data to make suggested changes in how the person engages in the physical activity.

Abstract: A measuring tape device may include a housing having an aperture, a reel assembly defining an axis, a blade having a first end configured to extend from the housing through the aperture and a second end configured to be wound on the reel assembly, a locking assembly configured to alternately allow and prevent winding of the blade onto the reel assembly based on a position of the locking assembly, and a guard assembly comprising guard members extending on opposing lateral sides of the locking assembly to protect the locking assembly from impact.

Abstract: A measuring tape device may include a housing having an aperture, a reel assembly defining an axis, a blade having a first end configured to extend from the housing through the aperture and a second end configured to be wound on the reel assembly, and a transverse aperture extending through the housing in a direction substantially parallel to the axis.

Abstract: The present disclosure provides systems and methods for providing training opportunities based on data collected from monitoring a physiological parameter of persons engaged in physical activity. The physical activity can be a sporting activity, such as a contact sport (e.g., football, hockey, lacrosse) or a recreational activity or sport (e.g., biking, hiking, skiing, snowboarding, motorsports). The system is configured with select components that perform a method of (i) recording data related to a physiological parameter of a person engaged in a physical activity (e.g., an impact received by a player engaged in a contact sport), (ii) analyzing the recorded data related to the physiological parameter while the person is engaged in a physical activity (e.g., is the received impact greater than a predetermined threshold), and (iii) providing post-physical activity analysis of the recorded data to make suggested changes in how the person engages in the physical activity.

Abstract: Provided herein are host cells or host cellular expression systems that express a membrane protein. Also, methods are provided that use such host cells or host cellular expression systems to produce higher amounts of the membrane proteins. Further, the cells or cellular systems can be used as tools for the functional characterization of membrane proteins, as well as for screening and drug discovery efforts targeting membrane proteins.

Abstract: Aspects of the invention relate to a method for inhibiting the growth of a microbe that expresses bacterial vitamin K epoxide reductase (bVKOR). The method involves contacting the bacterial cell with an effective amount of an agent that inhibits bVKOR. Agents include a drug, ligand or portion thereof, protein, polypeptide, small organic molecule, antisense nucleic acid, RNAi, or antibody. Examples of useful agents are a phenylpropanoid, a modified phenylpropanoid, a coumarin or modified coumarin. A particularly useful agents is warfarin or a variant thereof or ferulenol or a variant thereof. The microbe is any microbe carrying a bVKOR gene, such as Mycobacterium tuberculosis.

Abstract: The invention provides composition and methods for producing proteins of interest which comprise at least one disulfide bond, include proteins which in their mature form do not contain disulfide bonds, but whose precursor molecule contained at least one disulfide bond. The methods employ a host cell modified to more efficiently produce properly folded disulfide bond containing proteins. The host cells generally contain a mutation in one or more reductase genes, and can be further genetically modified to increase their growth rate, and are further optionally modified to increase the expression of a catalyst of disulfide bond formation. Host cells, methods for u sing such to produce proteins of interest, proteins of interest produced by these methods are within the scope of the invention.

Abstract: The invention provides composition and methods for producing proteins of interest which comprise at least one disulfide bond, include proteins which in their mature form do not contain disulfide bonds, but whose precursor molecule contained at least one disulfide bond. The methods employ a host cell modified to more efficiently produce properly folded disulfide bond containing proteins. The host cells generally contain a mutation in one or more reductase genes, and can be further genetically modified to increase their growth rate, and are further optionally modified to increase the expression of a catalyst of disulfide bond formation. Host cells, methods for using such to produce proteins of interest, proteins of interest produced by these methods are within the scope of the invention.

Abstract: The invention provides composition and methods for producing proteins of interest which comprise at least one disulfide bond, include proteins which in their mature form do not contain disulfide bonds, but whose precursor molecule contained at least one disulfide bond. The methods employ a host cell modified to more efficiently produce properly folded disulfide bond containing proteins. The host cells generally contain a mutation in one or more reductase genes, and can be further genetically modified to increase their growth rate, and are further optionally modified to increase the expression of a catalyst of disulfide bond formation. Host cells, methods for u sing such to produce proteins of interest, proteins of interest produced by these methods are within the scope of the invention.

Abstract: An adjustable wrench includes: a head having a first fixed jaw; a handle extending away from the head; a selectively positionable second jaw; an adjustment mechanism that includes a rotatable shaft, an actuator, and a gear train operatively coupling the shaft to the second jaw; the shaft having an external spiral groove and disposed at least partially in the handle; the actuator slidable relative to the handle and engaging the groove; first and second flanges spaced from one another and each circumferentially surrounding the shaft; and a retainer disposed between the flanges and engaging the shaft so as to limit movement of the shaft parallel to the handle longitudinal axis in two directions via abutment with the first and second flanges. Such a wrench may have a proof torque of at least the minimum level set forth in ASME B107.8, 2003 revision. Other wrenches are also disclosed.

Abstract: A hand operated file is provided with a thin ceramic coating of a transition metal nitride, such as vanadium nitride and/or zirconium nitride, on its teeth. The coating may be 20 um or less, thereby not altering the macro dimensions of the file. The coating may be applied using a vapor deposition process such as physical vapor deposition, and advantageously via a physical vapor deposition process where the file body does not exceed a temperature of about 300° F. The coating helps prevent galling, thereby increasing the useful life of the file.

Abstract: An adjustable wrench includes: a head having a first fixed jaw; a handle extending away from the head and having a longitudinal axis; a second jaw selectively positionable with respect the first jaw; an adjustment mechanism comprising a rotatable shaft, an actuator, and a gear train operatively coupling the shaft to the second jaw; the shaft having an external spiral groove and disposed at least partially in the handle; the actuator slidable relative to the handle and engaging the groove; first and second flanges spaced from one another; and a retainer disposed between the flanges and engaging the shaft so as to limit movement of the shaft parallel to the handle longitudinal axis in two directions via abutment with the first and second flanges. Such a wrench may have a proof torque of at least the minimum level set forth in ASME B107.8, 2003 revision.