Abstract: Provided herein are methods for determining the mislocalization of an analyte by capturing the analyte on an array and measuring the mislocalization distance.

Abstract: Provided herein are methods for determining the mislocalization of an analyte by capturing the analyte on an array and measuring the mislocalization distance.

Abstract: Methods for identifying a cell population secreting a biomolecule with desirable attributes are disclosed. The desirable attributes can include, for example, quantity and quality (e.g., minimal aggregation and/or desired configuration). Cell populations identified by the disclosed methods are more likely to successfully scale during production. The methods can include assessing multiple domains/binding sites of a complex biomolecule and/or the formation of aggregates by the biomolecules. Methods of assessing a secretion level for biomolecules having a wide range of molecular weights and method for enhancing loading of cells into chambers of a microfluidic device.

Abstract: The present disclosure describes the use of immune modulators to promote nerve growth and regeneration, particularly in the context of nerve deficit stemming from trauma and disease. In particular, the disclosure provides for the use of CXCR4 antagonists, STAT3 activators, and an agent that increases levels of nitric oxide, either alone or in any combination of these drugs, in surgery performed to treat nerve deficit conditions, especially peripheral nerve deficit conditions caused by cut injury or tear injury, the method especially useful in bridging nerve gaps of 3 cm or longer.

Abstract: The present disclosure describes the use of immune modulators to promote nerve growth and regeneration, particularly in the context of nerve deficit stemming from trauma and disease. In particular, the disclosure provides for the use of of CXCR4 antagonsists, STAT3 activators, and an agent that increase nitric oxide, alone or in combination, to treat nerve deficit conditions.

Abstract: The present disclosure describes the use of immune modulators to promote nerve growth and regeneration, particularly in the context of nerve deficit stemming from trauma and disease. In particular, the disclosure provides for the use of CXCR4 antagonsists, STAT3 activators, and an agent that increase nitric oxide, alone or in combination, to treat nerve deficit conditions.

Abstract: The present disclosure relates in some aspects to methods and compositions for light-controlled in situ surface patterning of a substrate using a Click chemistry reaction. In some embodiments, a method disclosed herein comprises a copper-catalyzed alkyne-azide (CuAAC) click chemistry reaction for photocontrollable ligation of nucleic acid molecules. A large diversity of barcodes can be created in molecules on the substrate via sequential rounds of light exposure, hybridization, and ligation.

Abstract: The present disclosure is based in part on probes, compositions, methods, and kits for simultaneous, multiplexed spatial detection and quantification of protein and/or nucleic acid expression in a user-defined region of a tissue, user-defined cell, and/or user-defined subcellular structure within a cell.

Abstract: Provided herein are methods for determining the mislocalization of an analyte by capturing the analyte on an array and measuring the mislocalization distance.

Abstract: The present disclosure describes the use of immune modulators to promote nerve growth and regeneration, particularly in the context of nerve deficit stemming from trauma and disease. In particular, the disclosure provides for the use of of CXCR4 antagonsists, STAT3 activators, and an agent that increase nitric oxide, alone or in combination, to treat nerve deficit conditions.

Abstract: Zebularine has hypomethylating activity, and can be used to inhibit, reverse, and/or reduce DNA methylation in vivo and in vitro. Methods are provided for treating methylation-linked conditions through the application of 2-pyrimidinone derivatives, such as Zebularine. Compositions, including pharmaceutical compositions, comprising such derivatives are also provided. Also provided are kits for use in inhibiting DNA methylation, which kits include an amount of a 2-pyrimidinone derivative.

Abstract: There is disclosed 103 novel methylation-altered DNA sequences (“marker sequences”) that have distinct methylation patterns in cancer, compared to normal tissue. In many instances, these marker sequences represent novel sequences not found in the GenBank data base, and none of these marker sequences have previously been characterized with respect to their methylation pattern in human cancers including, but not limited to those of bladder and prostate. These 103 sequences have utility as diagnostic, prognostic and therapeutic markers in the treatment of human cancer, and as reagents in kits for detecting methylated CpG-containing nucleic acids.

Abstract: There is disclosed 103 novel methylation-altered DNA sequences (“marker sequences”) that have distinct methylation patterns in cancer, compared to normal tissue. In many instances, these marker sequences represent novel sequences not found in the GenBank data base, and none of these marker sequences have previously been characterized with respect to their methylation pattern in human cancers including, but not limited to those of bladder and prostate. These 103 sequences have utility as diagnostic, prognostic and therapeutic markers in the treatment of human cancer, and as reagents in kits for detecting methylated CpG-containing nucleic acids.

Abstract: Zebularine has hypomethylating activity, and can be used to inhibit, reverse, and/or reduce DNA methylation in vivo and in vitro. Methods are provided for treating methylation-linked conditions through the application of 2-pyrimidinone derivatives, such as Zebularine. Compositions, including pharmaceutical compositions, comprising such derivatives are also provided. Also provided are kits for use in inhibiting DNA methylation, which kits include an amount of a 2-pyrimidinone derivative.

Abstract: A client computer program provides instructions for a processor to locate and retrieve rich media and HTML files for running in a three-dimensional graphical user interface. The program provides instructions for the processor to generate a three-dimensional (3D) graphical user interface on a display. Then, the processor generates a metaphorical user object for navigating and interacting in the three dimensions within the environment via navigational and interactional inputs, respectively, from a user. The processor also integrates an HTML object into the environment, and interacts the user object with the HTML object in response to an interactional input from the user. Preferably, the processor generates one or more additional user objects for additional users. Each of the additional user objects can also interact with the HTML object, the first user object and/or each other within the environment in response to interactional inputs from the additional users.

Abstract: The invention discloses a data transmission method for the portable communication apparatus, comprising plurality of data transmission steps and each data transmission step is used for implementation a graphic, image, video, or audio process, wherein each step can be at least used for transmitting the 1st header for setting a graphic, image, video, or audio command, the 2nd header for designating the graphic, image, video, or audio parameter information following the graphic, image, video, or audio command and the combination of the said graphic, image, video, or audio command information, and transmitting the graphic, image, video, or audio parameter of the graphic, image, video, or audio command. In particular, if the graphic, image, video, or audio command in this data transmission step is already transmitted in the preceding data transmission step, then, the step for transmitting the 1st header can be omitted.

Abstract: An automatic umbrella including an inside cap at the top of the umbrella shaft thereof, a top runner mounted around the umbrella shaft, an open control spring mounted between the inside cap and the top runner and compressed to preserve energy for opening the umbrella when the umbrella is collapsed, a collapse control spring compressed and retained in the compressed position by means of the operation of the hook of a locating device on the top end of the bottom shaft of the umbrella shaft, and the operation of the detent of a release control device inside the umbrella shaft, to preserve energy for collapsing the umbrella when the umbrella is opened, and a control block with a push plate moved vertically to actuate the release control device in automatically opening/closing the umbrella, and a bottom runner having a hook hooked on the umbrella shaft for allowing the umbrella to be operated manually when the hook of the bottom runner is released.

Abstract: An umbrella having a light source mounted inside the umbrella handle and controlled by a slide switch to give off light through a refractor around the umbrella shaft, wherein the umbrella shaft is a transparent, solid, polygonal rod having a refracting area for refracting the light of the light source toward the ribs and umbrella cover of the umbrella.

Abstract: A folding collapsible frame assembly for an automatic folding umbrella, which includes a lower shaft, an upper shaft made to slide in the upper shaft, a collapse control spring, a lock ring moved to lock the collapse control spring in the compressed condition as the umbrella is opened, a stretch control spring controlled by a bottom spring on the handle of the umbrella to automatically push the umbrella into the open condition, a guide control mechanism controlled by a control bottom on the handle of the umbrella to release the lock ring from the collapse control spring for allowing the umbrella to be collapsed automatically.