Patents by Inventor Jonathan Coffman
Jonathan Coffman has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20230340425Abstract: Methods for continuously inactivating virus during manufacture of a biological product are provided. The methods include steps of (1) combining (a) a composition including a biological product, and (b) a composition including a virus-inactivation reagent, to obtain (c) a treatment composition having a predetermined property for inactivation of a virus, (2) confirming that the treatment composition exhibits the predetermined property, (3) transferring the treatment composition to a treatment vessel that includes an inlet, an outlet, and a static mixer, the transferring occurring at the inlet, (4) incubating the treatment composition in the treatment vessel at a predetermined temperature while the treatment composition flows at a predetermined rate and contacts the static mixer, and (5) collecting the treatment composition from the treatment vessel at the outlet, wherein steps (1) to (5) are carried out continuously. Apparatuses and systems including such a treatment vessel are also provided.Type: ApplicationFiled: June 29, 2023Publication date: October 26, 2023Inventors: Jonathan COFFMAN, Jeff GOBY, Scott GODFREY, Raquel OROZCO, Jens Holger VOGEL
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Patent number: 11725191Abstract: Methods for continuously inactivating virus during manufacture of a biological product are provided. The methods include steps of (1) combining (a) a composition including a biological product, and (b) a composition including a virus-inactivation reagent, to obtain (c) a treatment composition having a predetermined property for inactivation of a virus, (2) confirming that the treatment composition exhibits the predetermined property, (3) transferring the treatment composition to a treatment vessel that includes an inlet, an outlet, and a static mixer, the transferring occurring at the inlet, (4) incubating the treatment composition in the treatment vessel at a predetermined temperature while the treatment composition flows at a predetermined rate and contacts the static mixer, and (5) collecting the treatment composition from the treatment vessel at the outlet, wherein steps (1) to (5) are carried out continuously. Apparatuses and systems including such a treatment vessel are also provided.Type: GrantFiled: August 23, 2019Date of Patent: August 15, 2023Assignee: Boehringer Ingelheim International GmbHInventors: Jonathan Coffman, Jeff Goby, Scott Godfrey, Raquel Orozco, Jens Holger Vogel
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Publication number: 20220356454Abstract: Methods for continuously inactivating a virus during manufacture of a protein are provided. Steps include (1) combining, at a single predetermined volumetric ratio, a composition including the protein, and a composition including a virus-inactivation reagent, to obtain a treatment composition having a predetermined property for inactivation of a virus; (2) transferring the treatment composition to a treatment vessel; (3) incubating the treatment composition in the treatment vessel at predetermined conditions; and (4) subjecting the treatment composition to a post-treatment processing operation. The single predetermined volumetric ratio has been selected to ensure that the predetermined property of the treatment composition is maintained across a range of concentrations predicted for the protein in the composition including the protein.Type: ApplicationFiled: June 30, 2022Publication date: November 10, 2022Inventors: Marcus Andre FIADEIRO, Jonathan COFFMAN, Robert Lee FAHRNER, Jill Ann KUBLBECK, Raquel OROZCO, Jeffrey Richard SALM
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Publication number: 20220340948Abstract: The invention relates to a serum-free cell culture perfusion medium comprising the medium components subgrouped into at least three separate aqueous concentrated feeds and a diluent, wherein the resulting serum-free cell culture perfusion medium is pH-adjusting to neutral pH upon mixing. Also provided is a method of preparing said serum-free cell culture perfusion medium. The invention further relates to methods of culturing mammalian cells or producing a protein of interest in perfusion culture using said serum-free cell culture perfusion medium that achieve high productivity at a low cell specific perfusion rate. The invention further relates to the use of the new and improved serum-free cell culture perfusion medium to control osmolality in a perfusion cell culture, wherein increasing osmolality results in an increase in total productivity and/or cell specific productivity by suppressing cell growth during cell culture, e.g., during production phase of perfusion cell culture.Type: ApplicationFiled: September 25, 2020Publication date: October 27, 2022Inventors: Jonathan COFFMAN, Henry LIN, Todd LUMAN, Daisie OGAWA, Janani RAVIKRISHNAN, Hayden TESSMAN, Samet YILDIRIM, Marcella YU
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Patent number: 11407981Abstract: Methods for continuously inactivating a virus during manufacture of a protein are provided. Steps include (1) combining, at a single predetermined volumetric ratio, a composition including the protein, and a composition including a virus-inactivation reagent, to obtain a treatment composition having a predetermined property for inactivation of a virus; (2) transferring the treatment composition to a treatment vessel; (3) incubating the treatment composition in the treatment vessel at predetermined conditions; and (4) subjecting the treatment composition to a post-treatment processing operation. The single predetermined volumetric ratio has been selected to ensure that the predetermined property of the treatment composition is maintained across a range of concentrations predicted for the protein in the composition including the protein.Type: GrantFiled: March 10, 2017Date of Patent: August 9, 2022Assignee: Boehringer Ingelheim International GmbHInventors: Marcus Andre Fiadeiro, Jonathan Coffman, Robert Lee Fahrner, Jill Ann Kublbeck, Raquel Orozco, Jeffrey Richard Salm
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Publication number: 20220162535Abstract: Integrated systems and methods for the manufacturing of recombinant proteins in mammalian cells are provided. The systems and methods include a single-use bioreactor (SUB), a tangential flow filtration system, a column chromatography skid, a tankless hold, a virus inactivation plug flow reactor, and pumps.Type: ApplicationFiled: March 27, 2020Publication date: May 26, 2022Inventors: Jonathan COFFMAN, Scott A. GODFREY, Raquel OROZCO, Robert Lee FAHRNER, Marcus Andre FIADEIRO, Robert E. KOTTMEIER, Joseph K. McLaughlin, Jeffrey Richard SALM
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Publication number: 20210388308Abstract: A continuous flow reactor having a plurality of interwoven flow paths in fluid communication to form a single continuous flow reactor tube having a single flow path.Type: ApplicationFiled: October 7, 2019Publication date: December 16, 2021Inventors: Raquel OROZCO, Jonathan COFFMAN, Stephanie PARKER, Natira Gnat MATTHEWS
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Publication number: 20210388323Abstract: A viral inactivation device including at least one experimental continuous viral inactivation reactor having at least an inlet, an outlet, and a tubular flow path and a computer system that, based on the experimental continuous viral inactivation reactor can design, select, make, and/or manufacture a scaled actual reactor. The tubular flow path includes a set of alternating turns that form a serpentine or an interwoven pattern between the inlet and the outlet.Type: ApplicationFiled: October 2, 2019Publication date: December 16, 2021Inventors: Matthew R. BROWN, Jonathan COFFMAN, Raquel OROZCO
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Publication number: 20210380914Abstract: A viral inactivation device including at least one continuous viral inactivation reactor having an inlet, an outlet, and a tubular flow path. The tubular flow path includes a set of alternating turns that form a serpentine pattern between the inlet and the outlet.Type: ApplicationFiled: October 7, 2019Publication date: December 9, 2021Inventors: Stephanie PARKER, Cameron Lee BARDLIVING, Jonathan COFFMAN, Scott GODFREY, Raquel OROZCO
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Publication number: 20210380923Abstract: The present invention provides improved bioprocessing systems and methods for cell culture using the improved bioreactors, e.g., batch-fed or perfusion bioreactor cell culture systems for production of monoclonal or bi-specific antibodies, which are modified to include one or more membrane gas transfer modules in place of a sparger- or microsparger-based aeration systems to better regulate the levels of critical gases in a bioreactor cell culture, e.g., the dissolved levels of O2 and CO2, even at high cell densities, without subjecting the cells to bubble-burst associated cell death.Type: ApplicationFiled: October 8, 2019Publication date: December 9, 2021Inventors: Jonathan COFFMAN, Scott GODFREY, Lisa SAWICKI
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Publication number: 20210317395Abstract: Methods of protein production in a linked culture and production bioreactor system are provided. Such methods include a culture bioreactor (N?1 bioreactor) linked to production bioreactor (N bioreactor). More specifically, the methods include (a) culturing cells with a gene that encodes the protein of interest in a continuous perfusion culture bioreactor (N?1 bioreactor); inoculating a continuously stirred tank reactor (CSTR) production bioreactor (N bioreactor) with cells obtained from step (a); and culturing the cells in the CSTR production bioreactor under conditions that allow production of the protein of interest.Type: ApplicationFiled: June 23, 2021Publication date: October 14, 2021Inventors: Gregory Walter HILLER, Matthew Paul GAGNON, Jonathan COFFMAN
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Patent number: 11104875Abstract: Methods of protein production in a linked culture and production bioreactor system are provided. Such methods include a culture bioreactor (N-1 bioreactor) linked to production bioreactor (N bioreactor). More specifically, the methods include (a) culturing cells with a gene that encodes the protein of interest in a continuous perfusion culture bioreactor (N-1 bioreactor); inoculating a continuously stirred tank reactor (CSTR) production bioreactor (N bioreactor) with cells obtained from step (a); and culturing the cells in the CSTR production bioreactor under conditions that allow production of the protein of interest.Type: GrantFiled: January 25, 2017Date of Patent: August 31, 2021Assignee: Boehringer Ingelheim International GmbHInventors: Gregory Walter Hiller, Matthew Paul Gagnon, Jonathan Coffman
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Publication number: 20200087632Abstract: Methods for continuously inactivating virus during manufacture of a biological product are provided. The methods include steps of (1) combining (a) a composition including a biological product, and (b) a composition including a virus-inactivation reagent, to obtain (c) a treatment composition having a predetermined property for inactivation of a virus, (2) confirming that the treatment composition exhibits the predetermined property, (3) transferring the treatment composition to a treatment vessel that includes an inlet, an outlet, and a static mixer, the transferring occurring at the inlet, (4) incubating the treatment composition in the treatment vessel at a predetermined temperature while the treatment composition flows at a predetermined rate and contacts the static mixer, and (5) collecting the treatment composition from the treatment vessel at the outlet, wherein steps (1) to (5) are carried out continuously. Apparatuses and systems including such a treatment vessel are also provided.Type: ApplicationFiled: August 23, 2019Publication date: March 19, 2020Inventors: Jonathan COFFMAN, Jeff GOBY, Scott GODFREY, Raquel OROZCO, Jens Holger VOGEL
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Patent number: 10435670Abstract: Methods for continuously inactivating virus during manufacture of a biological product are provided. The methods include steps of (1) combining (a) a composition including a biological product, and (b) a composition including a virus-inactivation reagent, to obtain (c) a treatment composition having a predetermined property for inactivation of a virus, (2) confirming that the treatment composition exhibits the predetermined property, (3) transferring the treatment composition to a treatment vessel that includes an inlet, an outlet, and a static mixer, the transferring occurring at the inlet, (4) incubating the treatment composition in the treatment vessel at a predetermined temperature while the treatment composition flows at a predetermined rate and contacts the static mixer, and (5) collecting the treatment composition from the treatment vessel at the outlet, wherein steps (1) to (5) are carried out continuously. Apparatuses and systems including such a treatment vessel are also provided.Type: GrantFiled: April 15, 2015Date of Patent: October 8, 2019Assignee: Boehringer Ingelheim International GmbHInventors: Jonathan Coffman, Jeff Goby, Scott Godfrey, Raquel Orozco, Jens Holger Vogel
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Publication number: 20190169559Abstract: The subject technology relates to a cell retention device and method for use in a perfusion cell culture system where the cell retention device includes a hollow-fiber filter having an average pore size ranging from about 0.5 to about 20 ?m and having the ability to operate under perfusion cell culture conditions for up to 35 days without being clogged or losing its product sieving ability by more than 20%.Type: ApplicationFiled: April 13, 2017Publication date: June 6, 2019Inventors: Jonathan COFFMAN, Scott GODFREY, Samantha WANG
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Publication number: 20190071647Abstract: Methods for continuously inactivating a virus during manufacture of a protein are provided. Steps include (1) combining, at a single predetermined volumetric ratio, a composition including the protein, and a composition including a virus-inactivation reagent, to obtain a treatment composition having a predetermined property for inactivation of a virus; (2) transferring the treatment composition to a treatment vessel; (3) incubating the treatment composition in the treatment vessel at predetermined conditions; and (4) subjecting the treatment composition to a post-treatment processing operation. The single predetermined volumetric ratio has been selected to ensure that the predetermined property of the treatment composition is maintained across a range of concentrations predicted for the protein in the composition including the protein.Type: ApplicationFiled: March 10, 2017Publication date: March 7, 2019Inventors: Marcus Andre FIADEIRO, Jonathan COFFMAN, Robert Lee FAHRNER, Jill Ann KUBLBECK, Raquel OROZCO, Jeffrey Richard SALM
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Publication number: 20190031997Abstract: Methods of protein production in a linked culture and production bioreactor system are provided. Such methods include a culture bioreactor (N-1 bioreactor) linked to production bioreactor (N bioreactor). More specifically, the methods include (a) culturing cells with a gene that encodes the protein of interest in a continuous perfusion culture bioreactor (N-1 bioreactor); inoculating a continuously stirred tank reactor (CSTR) production bioreactor (N bioreactor) with cells obtained from step (a); and culturing the cells in the CSTR production bioreactor under conditions that allow production of the protein of interest.Type: ApplicationFiled: January 25, 2017Publication date: January 31, 2019Inventors: Gregory Walter HILLER, Matthew Paul GAGNON, Jonathan COFFMAN
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Publication number: 20170037381Abstract: Methods for continuously inactivating virus during manufacture of a biological product are provided. The methods include steps of (1) combining (a) a composition including a biological product, and (b) a composition including a virus-inactivation reagent, to obtain (c) a treatment composition having a predetermined property for inactivation of a virus, (2) confirming that the treatment composition exhibits the predetermined property, (3) transferring the treatment composition to a treatment vessel that includes an inlet, an outlet, and a static mixer, the transferring occurring at the inlet, (4) incubating the treatment composition in the treatment vessel at a predetermined temperature while the treatment composition flows at a predetermined rate and contacts the static mixer, and (5) collecting the treatment composition from the treatment vessel at the outlet, wherein steps (1) to (5) are carried out continuously. Apparatuses and systems including such a treatment vessel are also provided.Type: ApplicationFiled: April 15, 2015Publication date: February 9, 2017Inventors: Jonathan COFFMAN, Jeff GOBY, Scott GODFREY, Raquel OROZCO, Jens Holger VOGEL
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Publication number: 20070066806Abstract: Separation methods, for example, to isolate a recombinant protein, are disclosed. In some implementations, a method includes forming a solid containing a first cation and a first anion in a medium containing a protein, and separating the solid from the protein.Type: ApplicationFiled: September 8, 2006Publication date: March 22, 2007Applicant: WyethInventors: Jonathan Coffman, Russell Shpritzer, Steven Vicik