Abstract: The present disclosure provides an analyte sensor comprising a working electrode, a sensing layer disposed on at least a portion of the working electrode, and a hydrophilic polyurethane membrane overcoating at least the sensing layer, wherein the hydrophilic polyurethane is aliphatic, aromatic, or both aliphatic and aromatic. The hydrophilic polyurethane membrane limits the transport of mass to the sensing layer without the need to be crosslinked. With such membrane, the analyte sensor can provide consistent analyte measurements over a temperature range of about 22-42° C. The present disclosure is further related to a method of forming an analyte sensor comprising providing a working electrode, disposing a sensing layer on at least a portion of the working electrode, and coating at least the sensing layer with a hydrophilic polyurethane membrane, wherein the hydrophilic polyurethane is aliphatic, aromatic, or both aliphatic and aromatic.

Abstract: A sensor control device and methods of making them are described. The sensor control device includes an electronics housing and a plug assembly. The electronics housing includes an upper shell matable to a lower mount having a skin-facing surface. The plug assembly is coupled to the electronics housing and includes a sensor module that has a sensor and a sharp module having a sharp. The plug assembly includes a base having a skin-facing surface and a plug portion comprising a lumen therethrough. At least a portion of a surface of the electronics housing or the plug assembly comprises an antimicrobial agent. The antimicrobial agent may be a metal and/or a metal oxide.

Abstract: An analyte sensor and methods and systems related thereto, where at least one or more of a lower portion of a sensor tail substrate, at least one electrode, a sensing element, a membrane, and an optional non-electrochemical functional layer comprise an antimicrobial quality. Asymmetric, double-sided adhesive binders suitable for adhering the analyte sensor to a skin surface comprising a non-woven scrim, a first pressure-sensitive adhesive layer, a second pressure-sensitive adhesive layer. Overbandages comprising a vapor-permeable backing, an aperture to circumferentially surround a sensor housing, a contact adhesive layer, first and second peelable release liners, and a third peelable release liner disposed upon an anchoring adhesive strip of the contact adhesive layer.

Abstract: Systems, devices and methods are provided for inserting at least a portion of an in vivo analyte sensor for sensing an analyte level in a bodily fluid of a subject. In particular, disclosed herein are various embodiments of applicators, and components thereof, designed to reduce trauma to tissue of a sensor insertion site and to increase the likelihood of a successful sensor insertion. Also disclosed are embodiments to ensure structural integrity of a sensor.

Abstract: The present disclosure relates to an analyte sensor comprising a working electrode, a sensing layer disposed on at least a portion of the working electrode that comprises an analyte-responsive enzyme, and a polymer membrane overcoating at least the sensing layer. The polymer membrane comprises an antimicrobial agent disposed therein, a hydrogel coating disposed thereon, or both an antimicrobial agent disposed therein and a hydrogel coating disposed thereon. The presence of an antimicrobial agent, such as an antibiotic, a hydrogel coating, or both reduces early/late signal attenuation of the analyte sensor. The polymer membrane can further be combined with a metal-containing layer in electrochemical communication with a reference electrode, a counter electrode, and/or second working electrode.

Abstract: A sensor control device and methods of making them are described. The sensor control device includes an electronics housing and a plug assembly. The electronics housing includes an upper shell matable to a lower mount having a skin-facing surface. The plug assembly is coupled to the electronics housing and includes a sensor module that has a sensor and a sharp module having a sharp. The plug assembly includes a base having a skin-facing surface and a plug portion comprising a lumen therethrough. At least a portion of a surface of the electronics housing or the plug assembly comprises an antimicrobial agent. The antimicrobial agent may be a metal and/or a metal oxide.

Abstract: The present disclosure provides analyte sensors comprising a sensing layer disposed upon a surface of a first working electrode, wherein the sensing layer comprises an NAD(P)-dependent enzyme and a supply of NAD(P); and a multilayered membrane that overcoats at least a part of the sensing layer and is permeable to an analyte, wherein the membrane comprises at least one layer of negatively charged polymer. The present disclosure also provides methods of using such analyte sensors for detecting one or more analytes preset in a biological sample and methods of manufacturing the analyte sensors.

Abstract: A system includes a sensor applicator, a sensor control device arranged within the sensor applicator and including an electronics housing and a sensor extending from a bottom of the electronics housing, and a cap coupled to one of the sensor applicator and the sensor control device, wherein the cap is removable prior to deploying the sensor control device from the sensor applicator.

Abstract: Analyte sensors responsive at low working electrode potentials may comprise an active area upon a surface of a working electrode, wherein the active area comprises a polymer, a redox mediator covalently bonded to the polymer, and at least one analyte-responsive enzyme covalently bonded to the polymer. A specific redox mediator responsive at low potential may have a structure of wherein G is a linking group covalently bonding the redox mediator to the polymer. A mass transport limiting membrane permeable to the analyte may overcoat the active area. In some sensor configurations, the mass transport limiting membrane may comprise a membrane polymer crosslinked with a branched crosslinker comprising three or more crosslinkable groups, such as polyethylene glycol tetraglycidyl ether.

Abstract: The present disclosure provides analyte sensors comprising a sensor tail substrate having a lower portion and configured for insertion into a tissue; a first working electrode disposed on the lower portion of the sensor tail substrate; a first sensing layer disposed upon a surface of the first working electrode; and a membrane disposed over at least the sensing layer; wherein the membrane comprises 20% to 50% by weight of a silver salt. The present disclosure also provides methods of using such analyte sensors for detecting one or more analytes present in a biological sample and methods of manufacturing the analyte sensors.

Abstract: The present disclosure provides an analyte sensor comprising a working electrode, a sensing layer disposed on at least a portion of the working electrode, and a hydrophilic polyurethane membrane overcoating at least the sensing layer, wherein the hydrophilic polyurethane is aliphatic, aromatic, or both aliphatic and aromatic. The hydrophilic polyurethane membrane limits the transport of mass to the sensing layer without the need to be crosslinked. With such membrane, the analyte sensor can provide consistent analyte measurements over a temperature range of about 22-42° C. The present disclosure is further related to a method of forming an analyte sensor comprising providing a working electrode, disposing a sensing layer on at least a portion of the working electrode, and coating at least the sensing layer with a hydrophilic polyurethane membrane, wherein the hydrophilic polyurethane is aliphatic, aromatic, or both aliphatic and aromatic.

Abstract: Methods and analyte sensors including a sensor tail comprising at least a first working electrode and a second working electrode that are spaced apart from one another along a length of the sensor tail. A first active area is disposed upon a surface of the first working electrode and a second active area is disposed upon a surface of the second working electrode, the first active area and the second active area being responsive to different analytes. A mass transport limiting membrane is deposited upon the first active area and the second active area by sequential dip coating operations, and the mass transport limiting membrane comprises a bilayer membrane portion overcoating the first active area and a homogeneous membrane portion overcoating the second active area.

Abstract: Analyte sensors responsive at low working electrode potentials may comprise an active area upon a surface of a working electrode, wherein the active area comprises a polymer, a redox mediator covalently bonded to the polymer, and at least one analyte-responsive enzyme covalently bonded to the polymer. A specific redox mediator responsive at low potential may have a structure of wherein G is a linking group covalently bonding the redox mediator to the polymer. A mass transport limiting membrane permeable to the analyte may overcoat the active area. In some sensor configurations, the mass transport limiting membrane may comprise a membrane polymer crosslinked with a branched crosslinker comprising three or more crosslinkable groups, such as polyethylene glycol tetraglycidyl ether.

Abstract: Analyte sensors are being increasingly employed for monitoring various analytes in vivo. Analyte sensors configured to monitor multiple analytes are also in development. Sufficient sensitivity for low-abundance analytes and multiple analytes having differing membrane permeability values may complicate analyte detection in some cases. Analyte sensors may feature enhancements to address one or both of these issues. Some analyte sensors may comprise a carbon working electrode comprising a dielectric substrate, one or more apertures extending through the dielectric substrate and filled with a carbon conductor pillar, a carbon conductor coating on a first face of the dielectric substrate in direct contact with each carbon conductor pillar, and one or more active areas on a second face of the dielectric substrate in electrical communication with the carbon conductor pillars.

Abstract: A system includes a sensor applicator, a sensor control device arranged within the sensor applicator and including an electronics housing and a sensor extending from a bottom of the electronics housing, and a cap coupled to one of the sensor applicator and the sensor control device, wherein the cap is removable prior to deploying the sensor control device from the sensor applicator.

Abstract: A system includes a sensor applicator, a sensor control device arranged within the sensor applicator and including an electronics housing and a sensor extending from a bottom of the electronics housing, and a cap coupled to one of the sensor applicator and the sensor control device, wherein the cap is removable prior to deploying the sensor control device from the sensor applicator.

Abstract: An analyte sensor and methods and systems related thereto, where at least one or more of a lower portion of a sensor tail substrate, at least one electrode, a sensing element, a membrane, and an optional non-electrochemical functional layer comprise an antimicrobial quality. Asymmetric, double-sided adhesive binders suitable for adhering the analyte sensor to a skin surface comprising a non-woven scrim, a first pressure-sensitive adhesive layer, a second pressure-sensitive adhesive layer. Overbandages comprising a vapor-permeable backing, an aperture to circumferentially surround a sensor housing, a contact adhesive layer, first and second peelable release liners, and a third peelable release liner disposed upon an anchoring adhesive strip of the contact adhesive layer.

Abstract: A system includes a sensor applicator, a sensor control device arranged within the sensor applicator and including an electronics housing and a sensor extending from a bottom of the electronics housing, and a cap coupled to one of the sensor applicator and the sensor control device, wherein the cap is removable prior to deploying the sensor control device from the sensor applicator.

Abstract: A system includes a sensor applicator, a sensor control device arranged within the sensor applicator and including an electronics housing and a sensor extending from a bottom of the electronics housing, and a cap coupled to one of the sensor applicator and the sensor control device, wherein the cap is removable prior to deploying the sensor control device from the sensor applicator.

Abstract: A sensor control device and methods of making them are described. The sensor control device includes an electronics housing and a plug assembly. The electronics housing includes an upper shell matable to a lower mount having a skin-facing surface. The plug assembly is coupled to the electronics housing and includes a sensor module that has a sensor and a sharp module having a sharp. The plug assembly includes a base having a skin-facing surface and a plug portion comprising a lumen therethrough. At least a portion of a surface of the electronics housing or the plug assembly comprises an antimicrobial agent. The antimicrobial agent may be a metal and/or a metal oxide.