Abstract: Methods and vitrification systems for biological samples are provided. The vitrification system has a rotatable cryowheel (210) with a facing surface (220). Droplets of a composition that includes a biological sample are released onto the facing surface. Droplets are rapidly pulled beneath the surface of the cryogenic coolant (160) to generate vitrified samples (180). Methods and cryopreservation devices are also provided that incorporate the vitrification systems.

Abstract: Methods and vitrification systems for biological samples are provided. The vitrification system has a rotatable cryowheel (210) with a facing surface (220). Droplets of a composition that includes a biological sample are released onto the facing surface. Droplets are rapidly pulled beneath the surface of the cryogenic coolant (160) to generate vitrified samples (180). Methods and cryopreservation devices are also provided that incorporate the vitrification systems.

Abstract: Haemodynamic parameters such as the amplitude and phase of a pulse wave passing through a region of interest can be obtained from a video image of the exposed skin of a patient by processing of the reflectance photoplethysmographic signal using signal averaging. The region of interest is defined and a reflectance photoplethysmographic signal obtained by finding the mean pixel intensity across the region of interest for each video frame. Signal averaging is performed on the resulting pulsatile waveform by detecting peaks in the waveform, selecting those parts of the waveform which lie within a window centered on the peaks, and summing the selected parts of the waveform to find an average pulse waveform. The region of interest is then divided into sub-regions and an average pulse waveform for the video sequence is found for each of the sub-regions in the same way.

Abstract: Haemodynamic parameters such as the amplitude and phase of a pulse wave passing through a region of interest can be obtained from a video image of the exposed skin of a patient by processing of the reflectance photoplethysmographic signal using signal averaging. The region of interest is defined and a reflectance photoplethysmographic signal obtained by finding the mean pixel intensity across the region of interest for each video frame. Signal averaging is performed on the resulting pulsatile waveform by detecting peaks in the waveform, selecting those parts of the waveform which lie within a window centred on the peaks, and summing the selected parts of the waveform to find an average pulse waveform. The region of interest is then divided into sub-regions and an average pulse waveform for the video sequence is found for each of the sub-regions in the same way.