Abstract: A histotripsy therapy system configured for the treatment of tissue is provided, which may include any number of features. Provided herein are systems and methods that provide efficacious non-invasive and minimally invasive therapeutic, diagnostic and research procedures. In particular, provided herein are optimized systems and methods that provide targeted, efficacious histotripsy in a variety of different regions and under a variety of different conditions without causing undesired tissue damage to intervening/non-target tissues or structures.

Abstract: Systems for analyzing multiple target analytes of a suspension include a tube and multiple floats. The target analytes are conjugated with at least one fluorescent marker, and the tube, multiple floats and suspension are centrifuged so that at least a portion of each target analyte is located between the inner wall of the tube and the surface of the respective float. In order to identify each target analyte, the portions of the suspension between the tube and each float is illuminated with one or more channels of excitation light, which causes the at least one fluorescent marker to become excited and emit light at longer wavelengths. One of the target analytes may be used to assess quality control or may aid in a subsequent analysis or diagnosis.