Abstract: A device for selective capture of target bladder cancer cells from urine or a urine derived fluid is provided. The device comprises a substrate having one or more cell capture surfaces, each cell capture surface comprising a functionalized film on the substrate and one or more target bladder cancer cell selective binding agents covalently bound to the functionalized film.

Abstract: A method of identifying an agent which modulates hydroxylation of hypoxia inducible factor (HIF), comprises contacting a HIF asparagine hydroxylase and a test substance in the presence of a substrate of the hydroxylase under conditions in which asparagine in the substrate is hydroxylated in the absence of the test substance; and determining hydroxylation of the substrate. Preferably the substrate is a HIF polypeptide comprising HIF-1?, a fragment thereof comprising Asn 803 of HIF-1? or a peptide analogue of HIF-1? or fragment thereof comprising an asparagine equivalent to Asn 803 of HIF-1? and wherein hydroxylation of Asn 803 or of a said equivalent asparagine is determined.