Patents by Inventor Jonathan L. Longmire

Jonathan L. Longmire has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Nucleic acid isolation process
    Patent number: 4921952
    Abstract: A method is provided for isolating DNA from eukaryotic cell and flow sorted chromosomes. When DNA is removed from chromosome and cell structure, detergent and proteolytic digestion products remain with the DNA. These products can be removed with organic extraction, but the process steps associated with organic extraction reduce the size of DNA fragments available for experimental use. The present process removes the waste products by dialyzing a solution containing the DNA against a solution containing polyethylene glycol (PEG). The waste products dialyze into the PEG leaving isolated DNA. The remaining DNA has been prepared with fragments containing more than 160 kb. The isolated DNA has been used in conventional protocols without affect on the protocol.
    Type: Grant
    Filed: April 20, 1989
    Date of Patent: May 1, 1990
    Assignee: The United States of America as represented by the United States Department of Energy
    Inventors: Jonathan L. Longmire, Annette K. Lewis, Carl E. Hildebrand