Patents by Inventor Jonathan Miles Brown

Jonathan Miles Brown has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Patent number: 11078484
    Abstract: The present invention relates to methods of administering to a subject multimeric oligonucleotides having monomeric subunits joined by linkers. The multimeric oligonucleotides have a molecular weight of at least about 45 kD and other characteristics, such that their clearance due to glomerular filtration is reduced. The present invention also relates to such multimeric oligonucleotides and methods of synthesizing such multimeric oligonucleotides.
    Type: Grant
    Filed: February 6, 2018
    Date of Patent: August 3, 2021
    Assignee: MPEG LA, LLC
    Inventors: Jonathan Miles Brown, Kristin K. H. Neuman, Hans-Peter Vornlocher, Philipp Hadwiger
  • Publication number: 20190085331
    Abstract: Defined multi-conjugate oligonucleotides can have predetermined sizes and compositions. For example, in various embodiment, defined multi-conjugate oligonucleotides can have advantageous properties, for example in the form of defined multi-conjugate siRNA (i.e., including two, three or more siRNA) having enhanced intracellular delivery and/or multi-gene silencing effects. In various embodiment, the defined multi-conjugate oligonucleotides can be synthesized via new synthetic intermediates and methods. The defined multi-conjugate oligonucleotides can be used, for example, in reducing gene expression, biological research, treating or preventing medical conditions, or to produce new or altered phenotypes in cells or organisms.
    Type: Application
    Filed: June 15, 2016
    Publication date: March 21, 2019
    Applicant: MPEG LA, LLC
    Inventors: Philipp Hadwiger, Hans-Peter Vornlocher, Jonathan Miles Brown, James Everett Dahlman, Kristin K. H. Neuman
  • Publication number: 20180223284
    Abstract: The present invention relates to methods of administering to a subject multimeric oligonucleotides having monomeric subunits joined by linkers. The multimeric oligonucleotides have a molecular weight of at least about 45 kD and other characteristics, such that their clearance due to glomerular filtration is reduced. The present invention also relates to such multimeric oligonucleotides and methods of synthesizing such multimeric oligonucleotides.
    Type: Application
    Filed: February 6, 2018
    Publication date: August 9, 2018
    Inventors: Kristin K. H. Neuman, Jonathan Miles Brown, Hans-Peter Vornlocher
  • Publication number: 20110309831
    Abstract: Protein structural determination using NMR techniques is improved through use of proteins in which one or more amino acids in the peptidic sequence are isotopically enriched in the sidechain with 2H and are isotopically enriched on the backbone with 13C, 15N, 2H or any combination thereof. This invention provides amino acids isotopically enriched as above, which can be used to synthesize isotopically labeled proteins and peptides for protein structural determinations by NMR, and methods for their synthesis. Other embodiments of the invention include peptidic molecules, media for peptidic molecule expression, methods of making isotopically labeled peptidic molecules and methods of determining structural information of a peptidic molecule.
    Type: Application
    Filed: March 15, 2011
    Publication date: December 22, 2011
    Applicant: ProSpect Pharma
    Inventors: Jonathan Miles Brown, Steven W. Homans, Michael Chaykovsky, Jenny Hong Murray
  • Patent number: 6376253
    Abstract: The invention is concerned with determining the three-dimensional structure of biological macromolecules such as proteins. In particular, it is concerned with methods for rapidly determining protein structure by NMR, by providing methods for simplifying NMR spectra using labeled proteins prepared from specifically isotopically labeled amino acids having at least two isotopes of 13C, 15N and 2H in the backbone, and methods for making these labeled proteins, e.g., by cultivation of a microbial culture containing said labeled amino acids.
    Type: Grant
    Filed: July 10, 2000
    Date of Patent: April 23, 2002
    Assignee: Martek Biosciences Corporation
    Inventors: Frank Elbert Anderson, III, Jonathan Miles Brown, Philip Edward Coughlin, Steven William Homans, Charles Tobias Weller, Carrie Ann Zimmerman-Wilson, Eddie James Oliver, Jr.
  • Patent number: 6340578
    Abstract: This invention is concerned with determining the three-dimensional structure of biological macromolecules such as proteins. In particular, it is concerned with methods for rapidly determining protein structure by NMR, by providing methods for simplifying NMR spectra using labeled proteins prepared from specifically isotopically labeled amino acids having at least two isotopes of 13C, 15N and 2H in the backbone, and methods for making these labeled proteins, e.g., by cultivation of a microbial culture containing said labeled amino acids.
    Type: Grant
    Filed: July 10, 2000
    Date of Patent: January 22, 2002
    Assignee: Martek Biosciences Corporation
    Inventors: Frank Elbert Anderson, III, Jonathan Miles Brown, Philip Edward Coughlin, Steven William Homans, Charles Tobias Weller, Carrie Ann Zimmerman-Wilson, Eddie James Oliver, Jr.
  • Patent number: 6335196
    Abstract: This invention is concerned with determining the three-dimensional structure of biological macromolecules such as proteins. In particular, it is concerned with methods for rapidly determining protein structure by NMR, by providing methods for simplifying NMR spectra using labeled proteins prepared from specifically isotopically labeled amino acids having at least two isotopes of 13C, 15N and 2H in the backbone, and methods for making these labeled proteins, e.g., by cultivation of a microbial culture containing said labeled amino acids.
    Type: Grant
    Filed: March 15, 2000
    Date of Patent: January 1, 2002
    Assignee: Martek Biosciences Corp.
    Inventors: Frank Elbert Anderson, III, Jonathan Miles Brown, Philip Edward Coughlin, Steven William Homans, Charles Tobias Weller, Carrie Ann Zimmerman-Wilson, Eddie James Oliver, Jr.
  • Patent number: 6111066
    Abstract: Peptidic molecules suitable for three dimensional NMR structure determination are provided which have been substituted with .sup.13 C and/or .sup.15 N in the backbone and/or at the .alpha.-carbon with .sup.2 H but not otherwise in the sidechains of the amino acid residues. One or more amino acids, amino acid species or substantially all of the amino acid residues of the molecule may be so substituted. A method of making the substituted peptidic molecules substituted in this manner is provided.
    Type: Grant
    Filed: September 2, 1997
    Date of Patent: August 29, 2000
    Assignee: Martek Biosciences Corporation
    Inventors: Frank Elbert Anderson, III, Jonathan Miles Brown, Philip Edward Coughlin, Steven William Homans, Charles Tobias Weller, Carrie Ann Zimmerman-Wilson
  • Patent number: 5817474
    Abstract: A method for determining three-dimensional structural information of a protein which involves producing the protein in a form substantially labeled with .sup.13 C of .sup.15 N or both substantially labeled with .sup.15 N and .sup.13 C and partially labeled with .sup.2 H and subjecting the protein to nuclear magnetic resonance spectroscopic analysis. The isotopically labeled protein is produced by a method which involves producing a substantially labeled microbial protein hydrolysate, subjecting the protein hydrolysate to cation exchange chromatography to produce a partially purified labeled amino acid mixture, subjecting the partially purified labeled amino acid mixture to anion exchange chromatography to produce a purified labeled amino mixture and supplementing the purified labeled amino acid mixture with isotopically labeled cysteine and optionally with isotopically labeled glutamine and asparagine.
    Type: Grant
    Filed: July 16, 1996
    Date of Patent: October 6, 1998
    Assignee: Martek Biosciences Corporation
    Inventor: Jonathan Miles Brown