Patents by Inventor Jonathan Minden
Jonathan Minden has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 11655271Abstract: Provided herein are methods, reagents, and kits for isolating polypeptides, such as a proteome. Also provided herein is a modified trypsin polypeptide that is resistant to autolysis, and that can be selectively-separated from a biological sample once digestion is complete.Type: GrantFiled: June 7, 2019Date of Patent: May 23, 2023Assignee: Carnegie Mellon UniversityInventors: Jonathan Minden, Amber Lucas
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Publication number: 20210253630Abstract: Provided herein are methods, reagents, and kits for isolating polypeptides, such as a proteome. Also provided herein is a modified trypsin polypeptide that is resistant to autolysis, and that can be selectively-separated from a biological sample once digestion is complete.Type: ApplicationFiled: June 7, 2019Publication date: August 19, 2021Inventors: Jonathan Minden, Amber Lucas
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Patent number: 10362237Abstract: The present disclosure provides systems and methods for the measurement of signal intensity across a large dynamic range. The systems disclosed herein employ an iterative image collection strategy that utilizes structured illumination to achieve greater than 1,000,000-fold dynamic range measurements, representing a dramatic improvement over the prior art.Type: GrantFiled: May 26, 2016Date of Patent: July 23, 2019Assignee: Carnegie Mellon UniversityInventors: Jonathan Minden, Frederick Lanni, Phu T Van
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Publication number: 20180167542Abstract: The present disclosure provides systems and methods for the measurement of signal intensity across a large dynamic range. The systems disclosed herein employ an iterative image collection strategy that utilizes structured illumination to achieve greater than 1,000,000-fold dynamic range measurements, representing a dramatic improvement over the prior art.Type: ApplicationFiled: May 26, 2016Publication date: June 14, 2018Inventors: Jonathan MINDEN, Frederick LANNI, Phu T VAN
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Patent number: 7598047Abstract: A process and a kit are provided for detecting differences in two or more samples of protein, including proteins bearing post-translational modifications and peptides. Proteins are prepared, for example, from each of a different group of cell samples or body fluid samples to be compared. Each protein extract is labeled with a different one of a luminescent dye from a matched set of dyes. The matched dyes have generally the same ionic and pH characteristics but emit light at different wavelengths to exhibit a different color upon luminescence detection. The labeled protein extracts are mixed together and separated together by electrophoresis or a chromatographic method. The separation is observed to detect proteins unique to one sample or present in a greater ratio in one sample than in the other. Those unique or excess proteins will fluoresce the color of one of the dyes used. Proteins common to each sample migrate together and fluoresce the same.Type: GrantFiled: November 14, 2003Date of Patent: October 6, 2009Assignee: Carnegie Mellon UniversityInventors: Jonathan Minden, Alan Waggoner, Susan Janet Fowler
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Patent number: 7566544Abstract: A process and a kit are provided for detecting differences in two or more samples of protein, including proteins bearing post-translational modifications and peptides. Proteins are prepared, for example, from each of a different group of cell samples or body fluid samples to be compared. Each protein extract is labeled with a different one of a luminescent dye from a matched set of dyes. The matched dyes have generally the same ionic and pH characteristics but emit light at different wavelengths to exhibit a different color upon luminescence detection. The labeled protein extracts are mixed together and separated together by electrophoresis or a chromatographic method. The separation is observed to detect proteins unique to one sample or present in a greater ratio in one sample than in the other. Those unique or excess proteins will fluoresce the color of one of the dyes used. Proteins common to each sample migrate together and fluoresce the same.Type: GrantFiled: May 1, 2002Date of Patent: July 28, 2009Assignee: Carnegie Mellon UniversityInventors: Jonathan Minden, Alan Waggoner, Susan Janet Fowler
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Publication number: 20070287828Abstract: Substrates (e.g., polymer), and/or solid supports (e.g., glass) having one or more biomolecule-binding compounds covalently bound to the surface of the substrate or solid support reversible covalent attachment of biomolecules thereto.Type: ApplicationFiled: February 18, 2005Publication date: December 13, 2007Inventor: Jonathan Minden
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Publication number: 20040161780Abstract: A process and a kit are provided for detecting differences in two or more samples of protein, including proteins bearing post-translational modifications and peptides. Proteins are prepared, for example, from each of a different group of cell samples or body fluid samples to be compared. Each protein extract is labeled with a different one of a luminescent dye from a matched set of dyes. The matched dyes have generally the same ionic and pH characteristics but emit light at different wavelengths to exhibit a different color upon luminescence detection. The labeled protein extracts are mixed together and separated together by electrophoresis or a chromatographic method. The separation is observed to detect proteins unique to one sample or present in a greater ratio in one sample than in the other. Those unique or excess proteins will fluoresce the color of one of the dyes used. Proteins common to each sample migrate together and fluoresce the same.Type: ApplicationFiled: November 14, 2003Publication date: August 19, 2004Inventors: Jonathan Minden, Alan Waggoner, Susan Janet Fowler
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Publication number: 20030054408Abstract: A method for identifying a protein including cleaving the protein with a proteolytic agent to produce peptide fragments, providing an array comprising a solution set of binding reagents, contacting the peptide fragments with the array to promote specific interactions between the fragments and the array, detecting the binding pattern of the peptide fragments on the array, and comparing the binding pattern of the peptide fragments to a reference set. A solution set refers to a set of binding reagents, or epitopes associated with such binding reagents, that can identify members of a given protein mixture or protein catalog using a minimal number of binding reagents (or epitopes corresponding to the binding reagents) based on certain constraints. The solution set can be determined using a randomized greedy algorithm. The solution set can be refined using a local search algorithm.Type: ApplicationFiled: April 22, 2002Publication date: March 20, 2003Inventors: Ramamoorthi Ravi, Bjarni Halldorsson, Alan Koretsky, Jonathan Minden
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Publication number: 20020177122Abstract: A process and a kit are provided for detecting differences in two or more samples of protein, including proteins bearing post-translational modifications and peptides. Proteins are prepared, for example, from each of a different group of cell samples or body fluid samples to be compared. Each protein extract is labeled with a different one of a luminescent dye from a matched set of dyes. The matched dyes have generally the same ionic and pH characteristics but emit light at different wavelengths to exhibit a different color upon luminescence detection. The labeled protein extracts are mixed together and separated together by electrophoresis or a chromatographic method. The separation is observed to detect proteins unique to one sample or present in a greater ratio in one sample than in the other. Those unique or excess proteins will fluoresce the color of one of the dyes used. Proteins common to each sample migrate together and fluoresce the same.Type: ApplicationFiled: May 1, 2002Publication date: November 28, 2002Inventors: Jonathan Minden, Alan Waggoner, Susan Janet Fowler
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Patent number: 6426190Abstract: A process and a kit are provided for detecting differences in two or more samples of protein, including proteins bearing post-translational modifications and peptides. Proteins are prepared, for example, from each of a different group of cell samples or body fluid samples to be compared. Each protein extract is labeled with a different one of a luminescent dye from a matched set of dyes. The matched dyes have generally the same ionic and pH characteristics but emit light at different wavelengths to exhibit a different color upon luminescence detection. The labeled protein extracts are mixed together and separated together by electrophoresis or a chromatographic method. The separation is observed to detect proteins unique to one sample or present in a greater ratio in one sample than in the other. Those unique or excess proteins will fluoresce the color of one of the dyes used. Proteins common to each sample migrate together and fluoresce the same.Type: GrantFiled: August 9, 1999Date of Patent: July 30, 2002Assignee: Carnegie Mellon UniversityInventors: Jonathan Minden, Alan Waggoner, Susan Janet Fowler
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Patent number: 6127134Abstract: A process and a kit are provided for detecting differences in two or more samples of protein. Protein extracts are prepared, for example, from each of a different group of cell samples to be compared. Each protein extract is labeled with a different one of a luminescent dye from a matched set of dyes. The matched dyes have generally the same ionic and pH characteristics but emit light at different wavelengths to exhibit a different color upon luminescence detection. The labeled protein extracts are mixed together and electrophoresed together. The gel is observed to detect proteins unique to one sample or present in a greater ratio in one sample than in the other. Those unique or excess proteins will fluoresce the color of one of the dyes used. Proteins common to each sample migrate together and fluoresce the same.Type: GrantFiled: April 20, 1995Date of Patent: October 3, 2000Assignee: Carnegie Mellon UniversityInventors: Jonathan Minden, Alan Waggoner
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Patent number: 6043025Abstract: A process and a kit are provided for detecting differences in two or more samples of protein. Protein extracts are prepared, for example, from each of a different group of cell samples to be compared. Each protein extract is labeled with a different one of a luminescent dye from a matched set of dyes. The matched dyes have generally the same ionic and pH characteristics but emit light at different wavelengths to exhibit a different color upon luminescence detection. The labeled protein extracts are mixed together and electrophoresed together. The gel is observed to detect proteins unique to one sample or present in a greater ratio in one sample than in the other. Those unique or excess proteins will fluoresce the color of one of the dyes used. Proteins common to each sample migrate together and fluoresce the same.Type: GrantFiled: October 10, 1997Date of Patent: March 28, 2000Assignee: Carnegie Mellon UniversityInventors: Jonathan Minden, Alan Waggoner