Abstract: A closure (20) for sealing a micro-organism container (50) is disclosed. The closure (20) includes a resilient seal (24) for sealing the container (50), a passageway (26) extending through the seal (24), or filter media (28) extending across the passageway (26) integrally molded to the seal (24) for allowing sterile gas exchange therethrough. A method of making a flask closure (20) is also disclosed. The method includes molding a sealing member (24) having a port (26) extending therethrough while simultaneously sealing a peripheral edge (31) of a filter media (28) within the passageway (26).

Abstract: A sealing member for a multi-well microfiltration plate comprises a flexible sealing material which, in response to the application of differential pressure, will flex or collapse in the direction of the filtration along the contour of each individual well. A process maintains a differential pressure substantially constant over a multi-well microfiltration plate comprising placing said flexible sealing member over that surface of the plate having well openings, stretching the flexible sealing member so as to seal the perimeter of each individual well, creating a differential pressure around said plate covered with said seal and filtering a media from each occupied well at a rate independent of the filtration rate of any other well while maintaining a substantially constant differential pressure around the plate until filtration in the last well containing media is complete.

Abstract: A sealing member for a multi-well microfiltration plate comprises a flexible sealing material which, in response to the application of differential pressure, will flex or collapse in the direction of the filtration along the contour of each individual well. A process maintains a differential pressure substantially constant over a multi-well microfiltration plate comprising placing said flexible sealing member over that surface of the plate having well openings, stretching the flexible sealing member so as to seal the perimeter of each individual well, creating a differential pressure around said plate covered with said seal and filtering a media from each occupied well at a rate independent of the filtration rate of any other well while maintaining a substantially constant differential pressure around the plate until filtration in the last well containing media is complete.