Patents by Inventor Jonathan N. Thon
Jonathan N. Thon has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 11850589Abstract: A system and method are provided for harvesting target biological substances. The system includes a substrate and a first and second channel formed in the substrate. The channels longitudinally extending substantially parallel to each other. A series of gaps extend from the first channel to the second channel to create a fluid communication path passing between a series of columns with the columns being longitudinally separated by a predetermined separation distance. The system also includes a first source configured to selectively introduce into the first channel a first biological composition at a first channel flow rate and a second source configured to selectively introduce into the second channel a second biological composition at a second channel flow rate. The sources are configured to create a differential between the first and second channel flow rates to generate physiological shear rates along the second channel that are bounded within a predetermined range.Type: GrantFiled: June 24, 2022Date of Patent: December 26, 2023Assignees: The Brigham and Women's Hospital, Inc., Boston, MA;, President and Fellows of Harvard College, Vilnius UniversityInventors: Joseph Italiano, Linas Mazutis, Jonathan N. Thon, David A. Weitz
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Publication number: 20230054335Abstract: There is provided a method of making a fluidic system that comprises assembling a fluidic system comprising a first plate, a second plate and a membrane disposed between the first plate and the second plate; applying laser energy to the fluidic system to cause the first plate, the second plate and the membrane to melt at bonding areas; and allowing the bonding areas to cool down such that the first plate, the second plate and the membrane are bonded together.Type: ApplicationFiled: August 19, 2022Publication date: February 23, 2023Applicant: Platelet Biogenesis, Inc.Inventors: Jorge Luis Valdez Macias, Marcus Lehmann, Jonathan N. Thon, Douglas G. Savin
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Patent number: 11566214Abstract: Systems and methods generating physiologic models that can produce functional biological substances are provided. In some aspects, a system includes a substrate and a first and second channel formed therein. The channels extend longitudinally and are substantially parallel to each other. A series of apertures extend between the first channel and second channel to create a fluid communication path passing through columns separating the channels that extends further along the longitudinal dimension than other dimensions. The system also includes a first source configured to selectively introduce into the first channel a first biological composition at a first channel flow rate and a second source configured to selectively introduce into the second channel a second biological composition at a second channel flow rate, wherein the first channel flow rate and the second channel flow rate create a differential configured to generate physiological shear rates within a predetermined range in the channels.Type: GrantFiled: February 17, 2020Date of Patent: January 31, 2023Assignees: BRIGHAM AND WOMEN'S HOSPITAL, INC., PRESIDENT AND FELLOWS OF HARVARD COLLEGE, VILNIUS UNIVERSITYInventors: Jonathan N. Thon, Joseph E. Italiano, Linas Mazutis, David A. Weitz
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Publication number: 20230009208Abstract: A system and method are provided for harvesting target biological substances. The system includes a substrate and a first and second channel formed in the substrate. The channels longitudinally extending substantially parallel to each other. A series of gaps extend from the first channel to the second channel to create a fluid communication path passing between a series of columns with the columns being longitudinally separated by a predetermined separation distance. The system also includes a first source configured to selectively introduce into the first channel a first biological composition at a first channel flow rate and a second source configured to selectively introduce into the second channel a second biological composition at a second channel flow rate. The sources are configured to create a differential between the first and second channel flow rates to generate physiological shear rates along the second channel that are bounded within a predetermined range.Type: ApplicationFiled: June 24, 2022Publication date: January 12, 2023Inventors: Joseph Italiano, Linas Mazutis, Jonathan N. Thon, David A. Weitz
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Publication number: 20210252070Abstract: Methods for producing megakaryocytes and platelets derived from inducible pluripotent stem cells and comprising a therapeutic agent are provided. The present disclosure further provides methods and compositions for loading a platelet or a megakaryocyte with a therapeutic agent and for genetically modifying a platelet or a megakaryocyte to express an agent.Type: ApplicationFiled: June 29, 2019Publication date: August 19, 2021Inventors: Jonathan N. Thon, Brad Dykstra, Brenden William Smith
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Publication number: 20210238523Abstract: The instant disclosure provides a system comprising a plurality of stacked bioreactors, wherein the system is configured to provide a substantially equal flow rate of fluid and pressure drop through each of the plurality of bioreactors. In some embodiments, the flow of the fluid through each of the plurality of bioreactors is configured to generate physiological shear rates to induce a biological source material in the bioreactors to produce target biological products.Type: ApplicationFiled: July 19, 2019Publication date: August 5, 2021Inventors: Jonathan N. Thon, Jorge Valdez, Marcus Lehmann, Douglas G. Sabin, Shawn Murphy, Shweta V. Krishnan
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Publication number: 20200255781Abstract: Systems and methods generating physiologic models that can produce functional biological substances are provided. In some aspects, a system includes a substrate and a first and second channel formed therein. The channels extend longitudinally and are substantially parallel to each other. A series of apertures extend between the first channel and second channel to create a fluid communication path passing through columns separating the channels that extends further along the longitudinal dimension than other dimensions. The system also includes a first source configured to selectively introduce into the first channel a first biological composition at a first channel flow rate and a second source configured to selectively introduce into the second channel a second biological composition at a second channel flow rate, wherein the first channel flow rate and the second channel flow rate create a differential configured to generate physiological shear rates within a predetermined range in the channels.Type: ApplicationFiled: February 17, 2020Publication date: August 13, 2020Inventors: Jonathan N. Thon, Joseph E. Italiano, Linas Mazutis, David A. Weitz
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Patent number: 10710073Abstract: A system and method are provided for harvesting target biological substances. The system includes a substrate and a first and second channel formed in the substrate. The channels longitudinally extending substantially parallel to each other. A series of gaps extend from the first channel to the second channel to create a fluid communication path passing between a series of columns with the columns being longitudinally separated by a predetermined separation distance. The system also includes a first source configured to selectively introduce into the first channel a first biological composition at a first channel flow rate and a second source configured to selectively introduce into the second channel a second biological composition at a second channel flow rate. The sources are configured to create a differential between the first and second channel flow rates to generate physiological shear rates along the second channel that are bounded within a predetermined range.Type: GrantFiled: May 24, 2019Date of Patent: July 14, 2020Assignees: Brigham and Women's Hospital, Inc., President and Fellows of Harvard College, Vilnius UniversityInventors: Joseph Italiano, Linas Mazutis, Jonathan N. Thon, David A. Weitz
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Publication number: 20200138868Abstract: Methods for producing megakaryocytes and platelets derived from inducible pluripotent stem cells are provided. Such megakaryocytes or platelets can be genetically modified to comprise a nucleic acid molecule encoding a therapeutic agent. The present disclosure further provides methods and compositions for loading a platelet or a megakaryocyte with a therapeutic agent and for genetically modifying a platelet or a megakaryocyte to express an agent.Type: ApplicationFiled: December 30, 2019Publication date: May 7, 2020Applicant: Platelet Biogenesis, Inc.Inventors: Jonathan N. Thon, Brad Dykstra, Brenden William Smith, Christian Gerald Peters, Thomas Carl Schulz
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Patent number: 10590373Abstract: Systems and methods generating physiologic models that can produce functional biological substances are provided. In some aspects, a system includes a substrate and a first and second channel formed therein. The channels extend longitudinally and are substantially parallel to each other. A series of apertures extend between the first channel and second channel to create a fluid communication path passing through columns separating the channels that extends further along the longitudinal dimension than other dimensions. The system also includes a first source configured to selectively introduce into the first channel a first biological composition at a first channel flow rate and a second source configured to selectively introduce into the second channel a second biological composition at a second channel flow rate, wherein the first channel flow rate and the second channel flow rate create a differential configured to generate physiological shear rates within a predetermined range in the channels.Type: GrantFiled: March 30, 2015Date of Patent: March 17, 2020Assignees: BRIGHAM AND WOMEN'S HOSPITAL, INC., PRESIDENT AND FELLOWS OF HARVARD COLLEGE, VILNIUS UNIVERSITYInventors: Jonathan N. Thon, Joseph E. Italiano, Linas Mazutis, David A. Weitz
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Publication number: 20190283027Abstract: A system and method are provided for harvesting target biological substances. The system includes a substrate and a first and second channel formed in the substrate. The channels longitudinally extending substantially parallel to each other. A series of gaps extend from the first channel to the second channel to create a fluid communication path passing between a series of columns with the columns being longitudinally separated by a predetermined separation distance. The system also includes a first source configured to selectively introduce into the first channel a first biological composition at a first channel flow rate and a second source configured to selectively introduce into the second channel a second biological composition at a second channel flow rate. The sources are configured to create a differential between the first and second channel flow rates to generate physiological shear rates along the second channel that are bounded within a predetermined range.Type: ApplicationFiled: May 24, 2019Publication date: September 19, 2019Inventors: Joseph Italiano, Linas Mazutis, Jonathan N. Thon, David A. Weitz
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Patent number: 10343163Abstract: A system and method are provided for harvesting target biological substances. The system includes a substrate and a first and second channel formed in the substrate. The channels longitudinally extending substantially parallel to each other. A series of gaps extend from the first channel to the second channel to create a fluid communication path passing between a series of columns with the columns being longitudinally separated by a predetermined separation distance. The system also includes a first source configured to selectively introduce into the first channel a first biological composition at a first channel flow rate and a second source configured to selectively introduce into the second channel a second biological composition at a second channel flow rate. The sources are configured to create a differential between the first and second channel flow rates to generate physiological shear rates along the second channel that are bounded within a predetermined range.Type: GrantFiled: September 20, 2017Date of Patent: July 9, 2019Assignees: The Brigham and Women's Hospital, Inc., President and Fellows of Harvard College, Vilnius UniversityInventors: Joseph Italiano, Linas Mazutis, Jonathan N. Thon, David A. Weitz
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Publication number: 20180334652Abstract: A system and method for generating biological products. In some aspects, the system includes a first substrate having formed therein a plurality of inlet channel extending substantially along a longitudinal direction, and a second substrate having formed therein a plurality of outlet channel corresponding to the plurality of inlet channel and extending substantially along the longitudinal direction, the second substrate configured to releasably engage the first substrate. The system also includes a permeable membrane, arranged between the substrates, forming microfluidic pathways between respective inlet and outlet channels and configured to selectively capture biological source material capable of generating biological products, wherein at least one channel is tapered transversally to control a pressure differential profile regulating perfusion through the permeable membrane.Type: ApplicationFiled: January 19, 2016Publication date: November 22, 2018Inventor: Jonathan N. Thon
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Publication number: 20180021780Abstract: A system and method are provided for harvesting target biological substances. The system includes a substrate and a first and second channel formed in the substrate. The channels longitudinally extending substantially parallel to each other. A series of gaps extend from the first channel to the second channel to create a fluid communication path passing between a series of columns with the columns being longitudinally separated by a predetermined separation distance. The system also includes a first source configured to selectively introduce into the first channel a first biological composition at a first channel flow rate and a second source configured to selectively introduce into the second channel a second biological composition at a second channel flow rate. The sources are configured to create a differential between the first and second channel flow rates to generate physiological shear rates along the second channel that are bounded within a predetermined range.Type: ApplicationFiled: September 20, 2017Publication date: January 25, 2018Inventors: Joseph Italiano, Linas Mazutis, Jonathan N. Thon, David A. Weitz
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Patent number: 9795965Abstract: A system and method are provided for harvesting target biological substances. The system includes a substrate and a first and second channel formed in the substrate. The channels longitudinally extending substantially parallel to each other. A series of gaps extend from the first channel to the second channel to create a fluid communication path passing between a series of columns with the columns being longitudinally separated by a predetermined separation distance. The system also includes a first source configured to selectively introduce into the first channel a first biological composition at a first channel flow rate and a second source configured to selectively introduce into the second channel a second biological composition at a second channel flow rate. The sources are configured to create a differential between the first and second channel flow rates to generate physiological shear rates along the second channel that are bounded within a predetermined range.Type: GrantFiled: November 20, 2013Date of Patent: October 24, 2017Assignee: Brigham and Women's Hospital, Inc.Inventors: Joseph Italiano, Linas Mazutis, Jonathan N. Thon, David A. Weitz
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Publication number: 20170183616Abstract: Systems and methods generating physiologic models that can produce functional biological substances are provided. In some aspects, a system includes a substrate and a first and second channel formed therein. The channels extend longitudinally and are substantially parallel to each other. A series of apertures extend between the first channel and second channel to create a fluid communication path passing through columns separating the channels that extends further along the longitudinal dimension than other dimensions. The system also includes a first source configured to selectively introduce into the first channel a first biological composition at a first channel flow rate and a second source configured to selectively introduce into the second channel a second biological composition at a second channel flow rate, wherein the first channel flow rate and the second channel flow rate create a differential configured to generate physiological shear rates within a predetermined range in the channels.Type: ApplicationFiled: March 30, 2015Publication date: June 29, 2017Inventors: Jonathan N. Thon, Joseph E. Italiano, Linas Mazutis, David A. Weitz
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Publication number: 20150336095Abstract: A system and method are provided for harvesting target biological substances. The system includes a substrate and a first and second channel formed in the substrate. The channels longitudinally extending substantially parallel to each other. A series of gaps extend from the first channel to the second channel to create a fluid communication path passing between a series of columns with the columns being longitudinally separated by a predetermined separation distance. The system also includes a first source configured to selectively introduce into the first channel a first biological composition at a first channel flow rate and a second source configured to selectively introduce into the second channel a second biological composition at a second channel flow rate. The sources are configured to create a differential between the first and second channel flow rates to generate physiological shear rates along the second channel that are bounded within a predetermined range.Type: ApplicationFiled: November 20, 2013Publication date: November 26, 2015Inventors: Joseph Italiano, Linas Mazutis, Jonathan N. Thon, David A. Weitz