Abstract: Hyperbranched cationic polymers are described. The polymers employ a 4-branching monomer resulting in an increase in the number of functional terminal groups due to the extra branching units, providing excellent transfection efficiency and cytocompatibility in different cell types, including aADSC, HeLa, Neu7 and RDEB keratinocytes, and delivering different genetic therapy approaches such GFP plasmid DNA and a ribonucleoprotein CRISP-Cas 9 complex for COL7A1 exon 80 skipping. In addition, the extra branching units of the polymer of the invention increases the positive charge on the polymer, which provides for improved endosomal escape within the cell. The 4-branching unit can be a diamine component, or a tetraacrylate component, although other 4-branching monomers may be employed such as for example any component with tetra acrylamide groups (i.e. 4-arm PEG acrylamide, 4-arm PEG maleimide), any component with tetra N- hydroxysuccinimide (NHS) groups (i.e.

Abstract: A nanoparticulate composition comprises a gene editing ribonucleoprotein system complexed within a cationic polymer. The cationic polymer may be a Poly-beta amino ester hyperbranched polymer, especially a 4-branching hyperbranched polymer. The gene editing ribonucleoprotein system may be a CRISPR-Cas9 gene editing system configured to excise a mutation or exon in a gene, replace a mutation in a gene, or produce a knock-down or knock-out of a gene, and in particular configured to excise exon 80 of the COL7A1 gene which codes for the collagen VII protein.

Abstract: A system and method for determining and reducing carbon emissions associated with the transportation of products around the world. An origin, a load, a discharge and a destination are entered on a computer interface. A processor determines carbon emissions for a plurality of routes from the origin to the destination using the origin, the load, the discharge, and the destination to determine and by considering one or more legs from the origin to the destination and by considering a plurality of modes of transportation for each of the legs. The processor determines which of the plurality of routes and which of the plurality of mode of transportations will generate the least carbon emission. A variety of parameters, including but not limited to, vehicle type, fuel time, arrival/departure times, can also be considered in determining carbon emissions. The route with the least carbon emissions can be graphically displayed. Additionally, other alternate routes can also be displayed.