Abstract: The present disclosure provides methods, compositions, kits and systems for nucleic acid amplification. In some embodiments, nucleic acid amplification methods include subjecting the nucleic acid to be amplified to partially denaturing conditions. In some embodiments, nucleic acid amplification methods include amplifying without fully denaturing the nucleic acid that is amplified. In some embodiments, the nucleic acid amplification method employs an enzyme that catalyzes homologous recombination and a polymerase. In some embodiments, methods for nucleic acid amplification can be conducted in a single reaction vessel and/or in a single continuous liquid phase of a reaction mixture, without need for compartmentalization of the reaction mixture or immobilization of reaction components.

Abstract: Fuel cell systems and related methods involving accumulators with multiple regions of differing water fill rates are provided. At least one accumulator region with a relatively more-rapid fill rate than another accumulator region is drained of water at shutdown under freezing conditions to allow at least that region to be free of water and ice. That region is then available to receive water from and supply water to, a fuel cell nominally upon start-up. The region having the relatively more-rapid fill rate may typically be of relatively lesser volume, and may be positioned either relatively below or relatively above the other region(s).

Abstract: Fuel cell systems and related methods involving accumulators with multiple regions of differing water fill rates are provided. At least one accumulator region with a relatively more-rapid fill rate than another accumulator region is drained of water at shutdown under freezing conditions to allow at least that region to be free of water and ice. That region is then available to receive water from and supply water to, a fuel cell nominally upon start-up. The region having the relatively more-rapid fill rate may typically be of relatively lesser volume, and may be positioned either relatively below or relatively above the other region(s).

Abstract: The present invention is embodied in a system for synchronizing a mobile device with video output by a video output device. In one embodiment, the system comprises a data reader, a remote control, and a router. The data reader is configured to be connected to a video output device for reading digital codes associated with video or audio signals output by the video output device. The remote control has a user input mechanism and is configured to transmit an activation signal to the data reader after activation of the user input mechanism. The router is configured to be connected to a wide area network for transmitting data from the data reader to a remote data server. The router is also configured to establish a local data connection between the data reader and a mobile device.