Abstract: A device, system and process involve conducting electroporation of microvesicles or exosomes or other target structures in a microfluidic arrangement at pressures that exceed atmospheric pressure. Single as well as multiple flow configurations can be employed. In some cases, the system and its operation are computer-controlled for partial or complete automation.

Abstract: Transfer of genetic and other materials to cells is conducted in a hands-free, automated, high throughput, continuous process. A system using a microfluidic hydrodynamic sheath flow configuration includes arrangements for pushing cells from side streams containing a cell culture medium to a central stream containing an electroporation buffer. Electroporation can be conducted in an assembly in which two or more microfluidic channels are provided in a parallel configuration and in which various layers can be stacked together to form a laminate type structure.

Abstract: A device, system and process involve conducting electroporation of microvesicles or exosomes or other target structures in a microfluidic arrangement at pressures that exceed atmospheric pressure. Single as well as multiple flow configurations can be employed. In some cases, the system and its operation are computer-controlled for partial or complete automation.

Abstract: Transfer of genetic and other materials to cells is conducted in a hands-free, automated, high throughput, continuous process. A system using a microfluidic hydrodynamic sheath flow configuration includes arrangements for pushing cells from side streams containing a cell culture medium to a central stream containing an electroporation buffer. Electroporation can be conducted in an assembly in which two or more microfluidic channels are provided in a parallel configuration and in which various layers can be stacked together to form a laminate type structure.

Abstract: According to various aspects and embodiments, a growth adaptive expandable stent is provided. The expandable stent includes a stent structure having a cylindrical shape that is self-expanding in a radial direction and includes a plurality of cylindrical rings disposed along a longitudinal axis of the stent structure. The stent structure is configured to exert a continuous outward radial force over time when implanted such that a diameter of the stent structure expands from a first value to a second value that is at least about 1.5 times the first value.

Abstract: According to various aspects and embodiments, a growth adaptive expandable stent is provided. The expandable stent includes a stent structure having a cylindrical shape that is self-expanding in a radial direction and includes a plurality of cylindrical rings disposed along a longitudinal axis of the stent structure. The stent structure is configured to exert a continuous outward radial force over time when implanted such that a diameter of the stent structure expands from a first value to a second value that is at least about 1.5 times the first value.

Abstract: Devices for treatment of cells are disclosed. The devices include an elongated housing and at least one hollow fiber semi-permeable membrane positioned within the housing having a plurality of pores dimensioned to prevent passage of the cells to be treated. Systems for treatment of cells including the device are disclosed. Methods of treating cells, including transducing cells and activating cells, are also disclosed. The methods include introducing a biosample with cells to be treated into the device, introducing media to suspend and release treated cells into the device, and discharging the treated cells from the device.

Abstract: A microchannel cell culture device is disclosed. The microchannel cell culture device includes a well plate defining an array of tissue modeling environments. A cell culture system including the microchannel cell culture device is also disclosed. The cell culture system includes a plurality of optical sensors, a platform, and a light source. A method of high throughput screening cell biological activity with the microchannel cell culture device is disclosed. A method of measuring oxygen consumption rate of cells in the microchannel cell culture device is disclosed. A method of facilitating drug development with the microchannel cell culture device is also disclosed.

Abstract: A method and system of delivering a charged cargo, such as a biomolecule, to a target structure, such as cells, exosomes, other vesicles or micelles, using an electroactive porous membrane. This method comprises contacting an electroactive porous membrane with a fluid flow toward the membrane. The fluid contains charged biomolecules and the membrane and biomolecules are oppositely charged so that the biomolecules in the fluid are trapped on the membrane as the fluid flows through the pores of the membrane. Acceptor cells of interest are pinned to the membrane by the flow of the fluid, thereby aggregating the cells onto the membrane in close proximity to the trapped biomolecules. Finally, the acceptor cells are permeabilized.

Abstract: According to various aspects and embodiments, a growth adaptive expandable stent is provided. The expandable stent includes a stent structure having a cylindrical shape that is self-expanding in a radial direction and includes a plurality of cylindrical rings disposed along a longitudinal axis of the stent structure. The stent structure is configured to exert a continuous outward radial force over time when implanted such that a diameter of the stent structure expands from a first value to a second value that is at least about 1.5 times the first value.

Abstract: The present disclosure describes systems and methods for providing culturing of a number of various tissue types in an air-liquid configuration in a high-throughput format and allowing co-culture of cells as well as application of physiologically relevant flow. A microfluidic cell culturing device is provided that includes a first channel having a first inlet port and a second inlet port, the first channel defined in a first layer. The microfluidic cell culturing device includes a membrane layer having a first surface coupled to the first layer defining the first channel, the membrane layer comprising semipermeable membrane that forms at least a portion of a surface of the first channel. The microfluidic cell culturing device includes a chamber defined in a second layer that exposes a portion the membrane layer to an external environment, wherein the chamber overlaps a portion of the first channel across the membrane layer.

Abstract: The methods and systems described herein provide a cell culture platform with an array of tissue modeling environments and dynamic control of fluid flow. The cell culture platform includes an array of wells that are fluidically coupled by microchannel structures. The dynamically controlled flow of fluid interacts with cells grown within the microchannels.

Abstract: The methods and systems described herein provide a cell culture platform with an array of tissue modeling environments and dynamic control of fluid flow. The cell culture platform includes an array of wells that are fluidically coupled by microchannel structures. The dynamically controlled flow of fluid interacts with cells grown within the microchannels.

Abstract: A system and method of using a microfluidic electroporation device for cell treatment is provided. The cell or exosome treatment system can include a microfluidic electroporation device, a voltage source coupled to a plurality of electrodes and a controller coupled to the voltage source. The microfluidic electroporation device can include a fluid receptacle, a semipermeable membrane, and a base including a channel in fluid communication with the fluid receptacle and the semipermeable membrane. A first electrode can be positioned within the fluid receptacle and a second electrode coupled to the base. The second electrode is positioned relative to the first electrode to create an electric field sufficient to electroporate cells or exosomes disposed in the fluid receptacle. The controller can be configured to cause the first and second electrodes to apply voltage electroporating the cells and exosomes.

Abstract: A device, system and process involve conducting electroporation of microvesicles or exosomes or other target structures in a microfluidic arrangement at pressures that exceed atmospheric pressure. Single as well as multiple flow configurations can be employed. In some cases, the system and its operation are computer-controlled for partial or complete automation.

Abstract: Transfer of genetic and other materials to cells is conducted in a hands-free, automated, high throughput, continuous process. A system using a microfluidic hydrodynamic sheath flow configuration includes arrangements for pushing cells from side streams containing a cell culture medium to a central stream containing an electroporation buffer. Electroporation can be conducted in an assembly in which two or more microfluidic channels are provided in a parallel configuration and in which various layers can be stacked together to form a laminate type structure.

Abstract: The present disclosure describes a system, device and method for differentiating cells such as, for example, generating ex vivo common lymphoid progenitors (CLPs) from human hematopoietic stem cells (HSCs). The system and method can be fully automated requiring minimal touch input from a user. Once harvested, the CLPs can be transplanted into a patient for cellular immune therapy.

Abstract: Electroporation is conducted in a system comprising a central fluid stream shielded by fluid streams having different electrical conductivities. The streams can be supported by microchannels. In one example, an inner sheath fluid flow is supported by microchannels at each side of a central microchannel. An outer sheath fluid is supported by outer microchannels at the exterior of the inner sheath fluid flow microchannels.

Abstract: A viral transduction and/or electroporation device has s a membrane separating two chambers and two electroporation electrodes for the chambers. An electrical voltage source is used for establishing an electrical field across the membrane and between the two electrodes. In operation, fluid is flowed into the chambers including fluid containing electroporation cargo and viral transduction solution and an electrical field is established across the membrane and between the electrodes to electroporate cells pinned to the membrane and transfecting the cells.

Abstract: A destroy on-demand electrical device includes a substrate layer formed using a soluble material (e.g., a Germanium oxide), a semi-conductor layer formed from a material that can become soluble upon further processing (e.g., Germanium) and conductive elements, formed from a metallic material such as Copper. The device is coupled with one or more disintegration sources that contain disintegration agents (e.g., Hydrogen Peroxide) that can promote disintegration of the device. The device can be destroyed in response to actuation of the disintegration sources, for example by actuation of a source that produces Hydrogen Peroxide for use in oxidizing the semi-conductor layer. Water can be used to dissolve dissolvable substrate layers. The semi-conductor layer can be destroyed by first processing this layer to form a dissolvable material and dissolving the processed layer with water. The remaining Copper components disintegrate once their underlying layer have been dissolved and/or by use of a salt.