Patents by Inventor Jonathan Rowley
Jonathan Rowley has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20210187032Abstract: Disclosed herein are compositions, kits, and methods involving hypertonic solutions. Specifically, disclosed herein are methods and compositions used for increasing the yield of extracellular material from cells. This occurs when said cells are exposed to hypertonic solutions.Type: ApplicationFiled: May 14, 2019Publication date: June 24, 2021Inventors: Jonathan A. ROWLEY, Kelvin S. NG, Lye Theng LOCK, Prarthana RAVISHANKAR
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Publication number: 20180216072Abstract: Disclosed herein are compositions and methods involving stem cells biopreserved on microcarriers, which can be thawed and expanded, all while maintaining their key attributes, such as their proliferative capacity, identity, functionality, and potency. Disclosed is a method for generating microcarriers-seeded-stem cells, as well as a post biopreservation procedure for thawing and inoculating bioreactors to achieve a rapid and scalable stem cells expansion.Type: ApplicationFiled: July 25, 2016Publication date: August 2, 2018Inventors: Lye Theng Lock, Jonathan Rowley
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Publication number: 20160317584Abstract: The present invention provides a fluid exchange cell culture technique and tissue repair cells (TRCs) made by these methods, as well as methods using these cells. The method includes a new wash step which increases the tissue repair properties of the TRCs of the invention. This wash step allows for the production of TRC populations with greater tissue repair and anti-inflammatory capabilities. Embodiments of the present invention include a post-culture process for cultured cells that preferably includes the steps of: a wash process for removing unwanted residual culture components, a volume reduction process, and a harvesting process to remove cultured cells. Preferably, all these steps are performed within a aseptically closed cell culture chamber by implementing a separation method that minimizes mechanical disruption of the cells and is simple to automate. The harvested cells may then be concentrated to a final volume for the intended use.Type: ApplicationFiled: July 12, 2016Publication date: November 3, 2016Inventors: Brian M. Hampson, Kristin Goltry, Douglas M. Smith, Jonathan A. Rowley, Naia Venturi
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Patent number: 9415071Abstract: The present invention provides a fluid exchange cell culture technique and tissue repair cells (TRCs) made by these methods, as well as methods using these cells. The method includes a new wash step which increases the tissue repair properties of the TRCs of the invention. This wash step allows for the production of TRC populations with greater tissue repair and anti-inflammatory capabilities. Embodiments of the present invention include a post-culture process for cultured cells that preferably includes the steps of: a wash process for removing unwanted residual culture components, a volume reduction process, and a harvesting process to remove cultured cells. Preferably, all these steps are performed within a aseptically closed cell culture chamber by implementing a separation method that minimizes mechanical disruption of the cells and is simple to automate. The harvested cells may then be concentrated to a final volume for the intended use.Type: GrantFiled: March 4, 2013Date of Patent: August 16, 2016Assignee: Vericel CorporationInventors: Brian Hampson, Kristin Goltry, Douglas M. Smith, Jonathan A. Rowley, Naia Venturi
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Patent number: 8394631Abstract: The present invention provides a fluid exchange cell culture technique and tissue repair cells (TRCs) made by these methods, as well as methods using these cells. The method includes a new wash step which increases the tissue repair properties of the TRCs of the invention. This wash step allows for the production of TRC populations with greater tissue repair and anti-inflammatory capabilities. Embodiments of the present invention include a post-culture process for cultured cells that preferably includes the steps of: a wash process for removing unwanted residual culture components, a volume reduction process, and a harvesting process to remove cultured cells. Preferably, all these steps are performed within a aseptically closed cell culture chamber by implementing a separation method that minimizes mechanical disruption of the cells and is simple to automate. The harvested cells may then be concentrated to a final volume for the intended use.Type: GrantFiled: December 7, 2010Date of Patent: March 12, 2013Assignee: Aastrom Biosciences, Inc.Inventors: Brian Hampson, Kristin Goltry, Douglas M. Smith, Jonathan A. Rowley, Naia Venturi
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Publication number: 20120294836Abstract: The present invention provides processes for aseptically processing live mammalian cells in an aqueous medium to produce a cell suspension having a cell density of at least about 10 million cells/mL and cell viability of at least about 90%. These methods comprise a step of reducing the volume of the medium using a tangential flow filter (TFF) having a pore size of greater than 0.1 micron, during which step the trans-membrane pressure (TMP) is maintained at less than about 3 psi and the shear rate is maintained at less than about 4000 sec?1. The invention also provides a complete process for large scale manufacturing mammalian cells for use in a therapeutic composition, and scalable, fully disposable systems for carrying out the process, using readily available disposables and pumps.Type: ApplicationFiled: July 19, 2012Publication date: November 22, 2012Applicant: LONZA WALKERSVILLE, INC.Inventors: Jonathan ROWLEY, Jacob PATTASSERIL, Ali MOHAMED
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Patent number: 8158122Abstract: The present invention provides a fluid exchange cell culture technique and tissue repair cells (TRCs) made by these methods, as well as methods using these cells. The method includes a new wash step which increases the tissue repair properties of the TRCs of the invention. This wash step allows for the production of TRC populations with greater tissue repair and anti-inflammatory capabilities. Embodiments of the present invention include a post-culture process for cultured cells that preferably includes the steps of: a wash process for removing unwanted residual culture components, a volume reduction process, and a harvesting process to remove cultured cells. Preferably, all these steps are performed within a aseptically closed cell culture chamber by implementing a separation method that minimizes mechanical disruption of the cells and is simple to automate. The harvested cells may then be concentrated to a final volume for the intended use.Type: GrantFiled: December 7, 2010Date of Patent: April 17, 2012Assignee: Aastrom Biosciences Inc.Inventors: Brian Hampson, Kristin Goltry, Douglas M. Smith, Jonathan A. Rowley, Naia Venturi
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Publication number: 20110287534Abstract: An apparatus and processes for aseptically dispensing live mammalian cells into sterile, flexible bags in a non-sterile atmosphere. The method includes the steps of: providing the cells suspended in a liquid; providing a plurality of sterile flexible bags fluidly connected to a main line by a plurality of branch lines of sterile flexible tubing; evacuating air from the flexible bags by applying a vacuum to the open end of the main line; preventing fluid flow through all branch lines except that of one bag to be filled; dispensing a desired volume of cell suspension into the open end of the main line; and introducing sufficient sterile purging gas under pressure into open end of the main line to drive into the bag any of the dispensed volume remaining in the main line or branch line of filled bag. Cells can be cryogenically preserved in the filled bags.Type: ApplicationFiled: May 3, 2011Publication date: November 24, 2011Applicant: LONZA WALKERSVILLE, INC.Inventors: Jonathan Rowley, Patrick Newsom
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Publication number: 20110124105Abstract: The present invention provides a fluid exchange cell culture technique and tissue repair cells (TRCs) made by these methods, as well as methods using these cells. The method includes a new wash step which increases the tissue repair properties of the TRCs of the invention. This wash step allows for the production of TRC populations with greater tissue repair and anti-inflammatory capabilities. Embodiments of the present invention include a post-culture process for cultured cells that preferably includes the steps of: a wash process for removing unwanted residual culture components, a volume reduction process, and a harvesting process to remove cultured cells. Preferably, all these steps are performed within a aseptically closed cell culture chamber by implementing a separation method that minimizes mechanical disruption of the cells and is simple to automate. The harvested cells may then be concentrated to a final volume for the intended use.Type: ApplicationFiled: December 7, 2010Publication date: May 26, 2011Applicant: Aastrom Biosciences, Inc.Inventors: Brian Hampson, Kristin Goltry, Doug Smith, Jonathan A. Rowley, Naia Venturi
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Publication number: 20110076294Abstract: The present invention provides a fluid exchange cell culture technique and tissue repair cells (TRCs) made by these methods, as well as methods using these cells. The method includes a new wash step which increases the tissue repair properties of the TRCs of the invention. This wash step allows for the production of TRC populations with greater tissue repair and anti-inflammatory capabilities. Embodiments of the present invention include a post-culture process for cultured cells that preferably includes the steps of: a wash process for removing unwanted residual culture components, a volume reduction process, and a harvesting process to remove cultured cells. Preferably, all these steps are performed within a aseptically closed cell culture chamber by implementing a separation method that minimizes mechanical disruption of the cells and is simple to automate. The harvested cells may then be concentrated to a final volume for the intended use.Type: ApplicationFiled: December 7, 2010Publication date: March 31, 2011Applicant: Aastrom Biosciences, Inc.Inventors: Brian Hampson, Kristin Goltry, Doug Smith, Jonathan A. Rowley, Naia Venturi
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Patent number: 7871605Abstract: The present invention provides a fluid exchange cell culture technique and tissue repair cells (TRCs) made by these methods, as well as methods using these cells. The method includes a new wash step which increases the tissue repair properties of the TRCs of the invention. This wash step allows for the production of TRC populations with greater tissue repair and anti -inflammatory capabilities. Embodiments of the present invention include a post-culture process for cultured cells that preferably includes the steps of: a wash process for removing unwanted residual culture components, a volume reduction process, and a harvesting process to remove cultured cells. Preferably, all these steps are performed within a aseptically closed cell culture chamber by implementing a separation method that minimizes mechanical disruption of the cells and is simple to automate. The harvested cells may then be concentrated to a final volume for the intended use.Type: GrantFiled: November 5, 2007Date of Patent: January 18, 2011Assignee: Aastrom Bioscience, Inc.Inventors: Brian Hampson, Kristin Goltry, Doug Smith, Jonathan A. Rowley, Naia Venturi
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Publication number: 20080200345Abstract: A high throughput method is provided for identifying agents capable of producing a desired biological response in whole cells. The method includes the steps of providing receptacles having a culture surface; placing different mixtures of single agents into selective ones of the receptacles according to a statistical design; and immobilizing the mixtures of single agents to the culture surface. The method further includes contacting the immobilized agents with the whole cells; and acquiring data which is indicative of a desired biological response in the contacted cells. The method also includes using statistical modeling of the acquired data to determine which mixtures of single agents and/or which single agents in these mixtures are effective in producing the desired biological response in the contacted cells.Type: ApplicationFiled: January 16, 2008Publication date: August 21, 2008Inventors: Andrea Liebmann-Vinson, Jonathan A. Rowley, Chris H. Bodily, Mohammad A. Heidaran
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Publication number: 20080175825Abstract: The present invention provides a fluid exchange cell culture technique and tissue repair cells (TRCs) made by these methods, as well as methods using these cells. The method includes a new wash step which increases the tissue repair properties of the TRCs of the invention. This wash step allows for the production of TRC populations with greater tissue repair and anti-inflammatory capabilities. Embodiments of the present invention include a post-culture process for cultured cells that preferably includes the steps of: a wash process for removing unwanted residual culture components, a volume reduction process, and a harvesting process to remove cultured cells. Preferably, all these steps are performed within a aseptically closed cell culture chamber by implementing a separation method that minimizes mechanical disruption of the cells and is simple to automate. The harvested cells may then be concentrated to a final volume for the intended use.Type: ApplicationFiled: November 5, 2007Publication date: July 24, 2008Inventors: Brian Hampson, Kristin Goltry, Doug Smith, Jonathan A. Rowley, Naia Venturi
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Publication number: 20060105455Abstract: Surfaces useful for cell culture comprise a support to which is bound a CAR material, and, bound to the CAR material, collagen VI or a biologically active fragment or variant thereof and, optionally, one or more other ECM proteins (or fragments or variants thereof) such as elastin, fibronectin, vitronectin, tenascin, laminin, entactin, aggrecan, decorin, collagen I, collagen III, and collagen IV. Also, optionally present on the surface is one or more polycationic polymers, such as poly-D-lysine or poly-D-ornithine. This surface is used in cell culture to promote cell attachment, survival, and/or proliferation of a number of different cell types such as (a) liver cells (e.g., HepG2 tumor cells, and a newly discovered line of rat liver epithelial stem cells) (b) osteoblasts, such as the murine cell line MC3T3 cell line and (c) primary bone marrow cells. Kits comprising the surfaces and additional reagents are also disclosed.Type: ApplicationFiled: December 30, 2005Publication date: May 18, 2006Applicant: Becton Dickinson and CompanyInventors: Richard Guarino, Jonathan Rowley, Andrea Liebmann-Vinson, John Hemperly, Mohammad Heidaran, Sharon Presnell
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Publication number: 20050265980Abstract: Compositions and methods for promoting mesenchymal stem cell expansion while maintaining a pluripotent phenotype are disclosed. Serum-free cell culture systems and kits and methods of use for mesenchymal stem cell expansion are provided. Methods also comprise the use of the expanded mesenchymal stem cells to treat various disorders or diseases, particularly those of the cardiovascular system, bone, or cartilage.Type: ApplicationFiled: May 13, 2005Publication date: December 1, 2005Applicant: Becton, Dickinson and CompanyInventors: Chang Chen, Andrea Liebmann-Vinson, Ruiling Xu, Jonathan Rowley, Perry Haaland, Bryce Chaney, Matthew Mitchell
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Publication number: 20050058687Abstract: Surfaces useful for cell culture comprise a support to which is bound a CAR material, and, bound to the CAR material, collagen VI or a biologically active fragment or variant thereof and, optionally, one or more other ECM proteins (or fragments or variants thereof) such as elastin, fibronectin, vitronectin, tenascin, laminin, entactin, aggrecan, decorin, collagen I, collagen III, and collagen IV. Also, optionally present on the surface is one or more polycationic polymers, such as poly-D-lysine or poly-D-ornithine. This surface is used in cell culture to promote cell attachment, survival, and/or proliferation of a number of different cell types such as (a) liver cells (e.g., HepG2 tumor cells, and a newly discovered line of rat liver epithelial stem cells) (b) osteoblasts, such as the murine cell line MC3T3 cell line and (c) primary bone marrow cells. Kits comprising the surfaces and additional reagents are also disclosed.Type: ApplicationFiled: September 12, 2003Publication date: March 17, 2005Applicant: Becton, Dickinson and CompanyInventors: Richard Guarino, Jonathan Rowley, Andrea Liebmann-Vinson, John Hemperly, Mohammad Heidaran, Sharon Presnell
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Publication number: 20050059083Abstract: A high throughput method is provided for identifying agents capable of producing a desired biological response in whole cells. The method includes the steps of providing receptacles having a culture surface; placing different mixtures of single agents into selective ones of the receptacles according to a statistical design; and immobilizing the mixtures of single agents to the culture surface. The method further includes contacting the immobilized agents with the whole cells; and acquiring data which is indicative of a desired biological response in the contacted cells. The method also includes using statistical modeling of the acquired data to determine which mixtures of single agents and/or which single agents in these mixtures are effective in producing the desired biological response in the contacted cells.Type: ApplicationFiled: September 15, 2003Publication date: March 17, 2005Inventors: Andrea Liebmann-Vinson, Jonathan Rowley, Chris Bodily, Mohammad Heidaran
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Publication number: 20040147016Abstract: The present invention relates to a programmable scaffold, which is a three-dimensional scaffold having interconnected pores and biologically active molecules physically entrapped therein. The scaffold is a lyophilized hydrogel of a polymer. The scaffold can be used in an array on a platform and loaded with various combinations of biologically active molecules for high throughput and parallel screening, as well as tissue engineering. The present invention also relates to methods for making and modifying the scaffolds.Type: ApplicationFiled: September 30, 2003Publication date: July 29, 2004Inventors: Jonathan A. Rowley, Mohammad A. Heidaran