Abstract: Methods, systems, and devices are provided for evaluating the status of cells in a sample involving imaging of cells, transformation of cell images into biophysical metrics, and transformation of the biophysical metrics into prognostic indications on the cellular and subject levels. Automated apparatus, processes, and analyses are provided according to present disclosure.

Abstract: Microfluidic devices for dissociating tissue, culturing, separating, manipulating, and assaying cells and methods for manufacturing and using the devices are disclosed. Individual modules for tissue dissociation, cell, protein and particle separation, cell adhesion to functionalized, permissive micro- and nano-substrates, cell culturing, cell manipulation, cell and extracellular component assaying via metabolic and therapeutic compounds are described. Specialized micro- and nano-substrates and their methods of fabrication are also described. An integrated device is also disclosed. The devices and methods can be used for diagnostic applications, monitoring of disease progression, analysis of disease recurrence, compound discovery, compound validation, drug efficacy screening, and cell-based assays.