Abstract: The present invention relates to a metal-organic framework composition, as well as constructs and methods thereof. In one particular example, the composition is employed to detect the presence of an acid gas.

Abstract: Disclosed herein is the finding that treatment with a ROCK inhibitor increases proliferation and induces immortalization of primary keratinocytes. Accordingly, provided is a method of immortalizing primary keratinocytes by exposure to a ROCK inhibitor. Also provided are immortalized primary keratinocytes produced by the described method, as well as organotypic tissue equivalents and cell cultures comprising the immortalized primary keratinocytes. Furthermore, ROCK inhibitor-treated cells show a greatly increased ability to support viral DNA replication of both “low risk” and “high risk” HPV genomes, indicating that ROCK inhibitors will be useful for studying the life cycles of a wide range of HPVs.

Abstract: Disclosed herein is the finding that treatment with a ROCK inhibitor increases proliferation and induces immortalization of primary keratinocytes. Accordingly, provided is a method of immortalizing primary keratinocytes by exposure to a ROCK inhibitor. Also provided are immortalized primary keratinocytes produced by the described method, as well as organotypic tissue equivalents and cell cultures comprising the immortalized primary keratinocytes. Furthermore, ROCK inhibitor-treated cells show a greatly increased ability to support viral DNA replication of both “low risk” and “high risk” HPV genomes, indicating that ROCK inhibitors will be useful for studying the life cycles of a wide range of HPVs.

Abstract: DNA transcription regulating proteins capable of binding to a DNA regulatory sequence that regulates expression of one or more environmental stress tolerance genes in a plant such as to cold and drought are provided. Cold-regulated transcription factors may include ZAT12 and RAV1. The present invention relates to the DNA regulatory sequence to enhance the stress tolerance of recombinant plants into which these genes are introduced. Methods for using the DNA and transcription regulating proteins to regulate expression of one or more native or non-native environmental stress tolerance genes in a plant are provided. Use of a promoter capable of effecting mRNA transcription in the selected plant cell to be transformed may also included. Other embodiments include the use of vectors.