Abstract: Described herein are methods of enhancing chromosomal homologous recombination to stimulate a loss of heterozygosity at a gene locus of interest in a living cell. These methods are driven by an enhancer component and a target-specific endonuclease component and proceed through a mechanism whereby: exogenous donor DNA that is homologous to the gene locus of interest is not introduced into the living cell; the desired allele of the gene locus of interest remains uncleaved; and the undesired allele is either uncleaved, cleaved at a single location, or cleaved at multiple locations. These methods have numerous applications, including the repair of risk alleles for disease prevention, the correction of heterozygous mutations in dividing cells, the design of cancer therapeutics, and the design of novel gene-drive strategies.

Abstract: Described herein are methods of enhancing chromosomal homologous recombination to stimulate a loss of heterozygosity at a gene locus of interest in a living cell. These methods are driven by an enhancer component and a target-specific endonuclease component and proceed through a mechanism whereby: exogenous donor DNA that is homologous to the gene locus of interest is not introduced into the living cell; the desired allele of the gene locus of interest remains uncleaved; and the undesired allele is either uncleaved, cleaved at a single location, or cleaved at multiple locations. These methods have numerous applications, including the repair of risk alleles for disease prevention, the correction of heterozygous mutations in dividing cells, the design of cancer therapeutics, and the design of novel gene-drive strategies.

Abstract: A carrier bolt assembly that is configured to provide an electrical charge to execute the firing of a round of ammunition. The carrier bolt assembly includes a carrier that includes a bore substantially therethrough. Further present is a void adjacent to the bore. A conductive rod is mateably formed to be operably coupled and fixedly secured within the bore and void. The conductive rod includes an outer layer and an inner layer wherein the inner layer is configured to conduct an electric current through the conductive rod. The conductive rod includes a second section contiguous with a first section and further includes a mounting block. The mounting block has a knob formed thereon that is operably coupled with the inner layer of the conductive rod. The carrier bolt assembly further includes an extractor pin that includes a tapered shaped wherein the extractor pin is tapered towards the midsection.

Abstract: A valve includes a valve body (100) disposable within a chamber (103) of a housing (101) and capable of being fluidly sealed with respect to the housing (101) when the valve is closed. At least one radial passage (117) is disposed in the valve body (100). At least one axial passage (119) is disposed in the valve body (100) in fluid communication with the at least one radial passage (117). When the valve at least partially opens, a fluid path opens between the valve body (100) and the housing (101), which fluid path (301) in is fluid communication with the at least one radial passage (117), and fluid from within the housing (101) is capable of passing out of the at least one axial passage (119).