Abstract: Systems and methods for producing organisms that lack one or more undesired traits are disclosed. In some implementations, a method includes obtaining (or producing) a first parent organism of a donor family of breeding stock and obtaining (or producing) a second parent organism from a receptor family of breeding stock. Some implementations of the method include mating the first parent organism with the second parent organism to produce a progeny organism. In some cases, a combination of genetic material from the first parent organism and the second parent organism in the progeny organism prevents development of the undesired trait (or of an organism having the undesired trait). Other implementations are also described.

Abstract: The disclosure generally relates to compositions, polynucleotides, kits, methods, and systems for generating clustered regularly interspaced short palindromic repeats (CRISPR) libraries. Disclosed are polynucleotides encoding for a constant region of a CRISPR single guide RNA (sgRNA) or CRISPR targeting RNA (crRNA) having a non-palindromic recognition site for a type II restriction enzyme oriented in a manner recognized by the type II restriction enzyme for cutting a site past an end of the polynucleotide. The methods include using the polynucleotide to prepare CRISPR libraries.

Abstract: The disclosure generally relates to compositions, polynucleotides, kits, methods, and systems for generating clustered regularly interspaced short palindromic repeats (CRISPR) libraries. Disclosed are polynucleotides encoding for a constant region of a CRISPR single guide RNA (sgRNA) or CRISPR targeting RNA (crRNA) having a non-palindromic recognition site for a type II restriction enzyme oriented in a manner recognized by the type II restriction enzyme for cutting a site past an end of the polynucleotide. The methods include using the polynucleotide to prepare CRISPR libraries.