Abstract: Topographically-guided cell migration devices include a substrate, an ion-permeable surface layer having a unidirectional textured first side, and an electrode pair having interdigitated electrode digits disposed between the substrate and the ion-permeable surface layer. The unidirectional texture of the ion-permeable surface layer is configured to guide migration of a cell culture in a substantially linear migration direction. Other topographically-guided cell migration devices include a substrate, a surface layer having a unidirectional textured first side, and a cell confinement structure with two walls that run substantially parallel to the unidirectional texture in order to guide migration of the cell culture in a substantially linear migration direction.