Patents by Inventor Jongyoon Han

Jongyoon Han has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Patent number: 10697964
    Abstract: This invention describes a one-step technique for the simultaneous label-free detection and concentration of blast cells from a blood sample. Enrichment of blast cells is achieved using a closed loop microfluidics system, allowing continuous removal of waste and non-target cells to generate concentrated samples of high purity without the need for specific targeting of proteins by antibodies. The technique is highly effective for samples which cannot be purified in a single run. The application of detecting rare blast cells for monitoring minimal residual disease in leukemia patients is demonstrated. The sensitivity of the invention promotes the detection of blast cells in blood samples of early-stage patients.
    Type: Grant
    Filed: October 6, 2017
    Date of Patent: June 30, 2020
    Assignee: Massachusetts Institute of Technology
    Inventors: Bee Luan Khoo, Jongyoon Han, Kyungyong Choi, Hyunryul Ryu
  • Patent number: 10697871
    Abstract: The present invention encompasses a micro-fluidic system having a closed-loop configuration in which inertial micro-fluidic separation of particles and/or cells is continuously repeated by feeding part of the output back to the input so that the purity and/or concentration of the particles and/or cell is maximized. The invention also includes methods of using the micro-fluidic system.
    Type: Grant
    Filed: October 6, 2017
    Date of Patent: June 30, 2020
    Assignee: Massachusetts Institute of Technology
    Inventors: Hyunryul Ryu, Kyungyong Choi, Jongyoon Han
  • Publication number: 20200171488
    Abstract: Described is a multi-dimensional double spiral (MDDS) microfluidic device comprising a first spiral microchannel and a second microchannel, wherein the wherein the first spiral microchannel and second spiral microchannel have different cross-sectional areas. Also described is a device comprising a multi-dimensional double spiral and system for recirculation. The invention also encompasses methods of separating particles from a sample fluid comprising a mixture of particles comprising the use of the multi-dimensional double spiral microfluidic device.
    Type: Application
    Filed: November 14, 2019
    Publication date: June 4, 2020
    Inventors: Jongyoon Han, Hyungkook Jeon
  • Patent number: 10640744
    Abstract: Scalable, high throughput and power-efficient electromechanical lysis using low electric potential, which can be used for harvesting valuable intracellular biomolecules (DNA, RNA, and proteins) and metabolites (e.g., biodiesels, bioplastics, antibiotics, and antibodies), and for sterilizing large volume solutions (e.g. disinfection of bacterial contaminated drinking water). The method can be directly integrated with other microfluidic devices for all-in-one, fully integrated total-analysis systems for various bacterial (and cellular) studies and clinical applications.
    Type: Grant
    Filed: October 10, 2017
    Date of Patent: May 5, 2020
    Assignee: Massachusetts Institute of Technology
    Inventors: Minseok Kim, Jongyoon Han
  • Patent number: 10429467
    Abstract: In accordance with one aspect of this disclosure, there is provided a device for performing magnetic resonance relaxometry. The device comprises a radio-frequency spectrometer comprising at least one field-programmable gate array chip; a power amplifier electrically connected with the radio-frequency spectrometer and amplifying an electrical output of the radio-frequency spectrometer, thereby producing an amplified electrical signal comprising between about 0.
    Type: Grant
    Filed: September 25, 2014
    Date of Patent: October 1, 2019
    Assignees: MASSACHUSETTS INSTITUTE OF TECHNOLOGY, NANYANG TECHNOLOGICAL UNIVERSITY
    Inventors: Weng Kung Peng, Jongyoon Han, Lan Chen
  • Patent number: 10393684
    Abstract: A low-cost and bench-top magnetic resonance relaxometer can be used for ex-vivo biochemical stress tests on plasma/erythrocytes, enabling deep-phenotyping of an individual's oxidative status, susceptibility and capacity.
    Type: Grant
    Filed: April 23, 2016
    Date of Patent: August 27, 2019
    Assignees: Massachusetts Institute of Technology, National University Hospital (S) Pte
    Inventors: Weng Kung Peng, Jongyoon Han, Tze Ping Loh
  • Patent number: 10252924
    Abstract: A water stream is passed between two juxtaposed similar ion exchange membranes (AEMs or CEMs), forming an ion depletion and ion enrichment zones when an electric field is applied. As cations are selectively transferred through the CEMs, for example, anions are relocated in order to achieve electro-neutrality, resulting in the concentration drop (increase) in ion depletion (enrichment) zone. Trifurcation of the output channel allows collection of concentrated, dilute and intermediate streams, with the intermediate stream serving as input to the next stage of a serialized implementation.
    Type: Grant
    Filed: October 23, 2015
    Date of Patent: April 9, 2019
    Assignee: Massachusetts Institute of Technology
    Inventors: Bumjoo Kim, Jongyoon Han, Rhokyun Kwak, Bader Shafaqa Al-Anzi
  • Patent number: 10228313
    Abstract: In accordance with an embodiment of the invention, there is provided a method for: a) high-throughput, multiplexed, affinity-based separation of proteins—especially low abundance proteins—from complex biological mixtures such as serum; and b) high-throughput, multiplexed, affinity-based separation of cells—especially rare cells—from complex biological mixtures such as blood or blood fractions. The separation of proteins or cells is achieved based on differential binding to affinity-capture beads of different sizes and then sorting the protein-bound or cell-bound beads using the concept of centrifugal-induced Dean migration in a spiral microfluidic device. This method enables continuous-flow, high throughput affinity-separation of milligram-scale protein samples or millions of cells in minutes after binding.
    Type: Grant
    Filed: October 23, 2015
    Date of Patent: March 12, 2019
    Assignees: Massachusetts Institute of Technology, The Massachusetts General Hospital
    Inventors: Aniruddh Sarkar, Han Wei Hou, Jongyoon Han, Galit Alter
  • Publication number: 20180371400
    Abstract: Scalable, high throughput and power-efficient electromechanical lysis using low electric potential, which can be used for harvesting valuable intracellular biomolecules (DNA, RNA, and proteins) and metabolites (e.g., biodiesels, bioplastics, antibiotics, and antibodies), and for sterilizing large volume solutions (e.g. disinfection of bacterial contaminated drinking water). The method can be directly integrated with other microfluidic devices for all-in-one, fully integrated total-analysis systems for various bacterial (and cellular) studies and clinical applications.
    Type: Application
    Filed: October 10, 2017
    Publication date: December 27, 2018
    Inventors: Minseok Kim, Jongyoon Han
  • Patent number: 10099945
    Abstract: Between two juxtaposed similar ion exchange membranes (AEMs or CEMs), an ion depletion zone and ion enrichment zone are generated under an electric field. As cations are selectively transferred through the CEMs, for example, anions are relocated in order to achieve electro-neutrality, resulting in the concentration drop (increase) in ion depletion (enrichment) zone. The use of a sacrificial metal anode allows electrocoagulation (EC) concurrently with ICP thereby permitting concentration of both ionic and non-ionic impurities to occur at the same time within the same cell or device.
    Type: Grant
    Filed: November 17, 2017
    Date of Patent: October 16, 2018
    Assignee: Massachusetts Institute of Technology
    Inventors: Siwon Choi, Jongyoon Han
  • Patent number: 10098581
    Abstract: A system includes a microfluidic device configured to isolate one or more particles from a mixture, a flow rate matching device configured to match flow rate of the microfluidic device with flow rate of an electrical measurement device configured to measure an electrical property of the isolated particles, and an electrical measurement device configured to measure an electrical property of the isolated particles.
    Type: Grant
    Filed: January 16, 2015
    Date of Patent: October 16, 2018
    Assignee: Massachusetts Institute of Technology
    Inventors: Joel Voldman, Hao-Wei Su, Javier Lopez Prieto, Jongyoon Han, Lidan Wu
  • Patent number: 10077462
    Abstract: A method and microfluidic device useful for isolating microbes from a blood sample which includes introducing the blood sample into the sample inlet of a spiral microfluidic device; and introducing a second fluid into the sheath inlet of the microfluidic device, wherein the spiral channel terminates in a microbe outlet and a waste outlet, and wherein the spiral channel includes a length, height, and a width that define an aspect ratio adapted to isolate any microbes present in the sample along a first portion of the spiral channel terminating at the microbe outlet, and to isolate red blood cells and leukocytes along a second portion of the spiral channel terminating at the waste outlet; and collecting the microbes from the microbe outlet, thereby isolating the microbes.
    Type: Grant
    Filed: March 14, 2014
    Date of Patent: September 18, 2018
    Assignees: The Broad Institute, Inc., Massachusetts Institute of Technology, The General Hospital Corporation
    Inventors: Han Wei Hou, Jongyoon Han, Roby Bhattacharyya, Deb Hung
  • Publication number: 20180238915
    Abstract: The invention relates to generally applicable methods and platforms for the assessment of a wide variety of drugs, including biologic drugs. Aspects of the invention further relate to such methods and platforms capable of measuring not only the equilibria but also the kinetics of drug-receptor interactions. Such methods and platforms can be based on mobility-based assays, wherein species are separated along at least one separation dimension. By modulating the mobility, e.g., electrophoretic mobility, of at least one of a drug and a receptor, the separation of drug, receptor, and drug-receptor complex can be optimized, e.g., by enhancing spatial separation of one or more of the foregoing species from the others.
    Type: Application
    Filed: August 18, 2016
    Publication date: August 23, 2018
    Inventors: Wei Ouyang, Jongyoon Han
  • Publication number: 20180141832
    Abstract: Between two juxtaposed similar ion exchange membranes (AEMs or CEMs), an ion depletion zone and ion enrichment zone are generated under an electric field. As cations are selectively transferred through the CEMs, for example, anions are relocated in order to achieve electro-neutrality, resulting in the concentration drop (increase) in ion depletion (enrichment) zone. The use of a sacrificial metal anode allows electrocoagulation (EC) concurrently with ICP thereby permitting concentration of both ionic and non-ionic impurities to occur at the same time within the same cell or device.
    Type: Application
    Filed: November 17, 2017
    Publication date: May 24, 2018
    Inventors: Siwon Choi, Jongyoon Han
  • Publication number: 20180136210
    Abstract: This invention describes a one-step technique for the simultaneous label-free detection and concentration of blast cells from a blood sample. Enrichment of blast cells is achieved using a closed loop microfluidics system, allowing continuous removal of waste and non-target cells to generate concentrated samples of high purity without the need for specific targeting of proteins by antibodies. The technique is highly effective for samples which cannot be purified in a single run. The application of detecting rare blast cells for monitoring minimal residual disease in leukemia patients is demonstrated. The sensitivity of the invention promotes the detection of blast cells in blood samples of early-stage patients.
    Type: Application
    Filed: October 6, 2017
    Publication date: May 17, 2018
    Inventors: Bee Luan Khoo, Jongyoon Han, Kyungyong Choi, Hyunryul Ryu
  • Publication number: 20180128723
    Abstract: The present invention encompasses a micro-fluidic system having a closed-loop configuration in which inertial micro-fluidic separation of particles and/or cells is continuously repeated by feeding part of the output back to the input so that the purity and/or concentration of the particles and/or cell is maximized. The invention also includes methods of using the micro-fluidic system.
    Type: Application
    Filed: October 6, 2017
    Publication date: May 10, 2018
    Inventors: Hyunryul Ryu, Kyungyong Choi, Jongyoon Han
  • Patent number: 9880122
    Abstract: An electrochemical system with reduced limiting-current behavior is disclosed. The electrochemical system is useful for fuel cells and bio-sensors. In part, the invention relates a method of reducing or eliminating limiting-current behavior in the operation electrochemical systems, in particular those with ion-selective membrane or electrochemical electrodes, by spatially reducing the convection near the membrane or the electrode. The invention further relates to electrochemical systems in which micropores, microarrays or pillar arrays are used to reduce convection in comparison to conventional systems without microarrays, micropores or pillar arrays.
    Type: Grant
    Filed: May 1, 2014
    Date of Patent: January 30, 2018
    Assignee: Massachusetts Institute of Technology
    Inventors: Sung Hee Ko, Sung Jae Kim, Jongyoon Han, HiongYap Gan
  • Patent number: 9850146
    Abstract: Between two juxtaposed similar ion exchange membranes (AEMs or CEMs), an ion depletion zone and ion enrichment zone are generated under an electric field. As cations are selectively transferred through the CEMs, for example, anions are relocated in order to achieve electro-neutrality, resulting in the concentration drop (increase) in ion depletion (enrichment) zone. The use of a sacrificial metal anode allows electrocoagulation (EC) concurrently with ICP thereby permitting concentration of both ionic and non-ionic impurities to occur at the same time within the same cell or device.
    Type: Grant
    Filed: September 17, 2015
    Date of Patent: December 26, 2017
    Assignee: Massachusetts Institute of Technology
    Inventors: Siwon Choi, Jongyoon Han
  • Patent number: 9845252
    Abstract: Between two juxtaposed similar ion exchange membranes (AEMs or CEMs), an ion depletion zone (dde) and ion enrichment zone (den) are generated under an electric field. As cations are selectively transferred through the CEMs, for example, anions are relocated in order to achieve electro-neutrality, resulting in the concentration drop (increase) in ion depletion (enrichment) zone. The concentration drop (i.e. salt removal) is low and spatially gradual at relatively low voltage or current (i.e. Ohmic regime). However, at higher voltage or current (i.e. overlimiting regime), strong electroconvective vortex accelerates cation transport through CEMs, allowing us to “relocate” most salt ions. The flat depletion zone occurs with significantly low ion concentration, and corresponding strong electric field in the zone, and any charged agents (e.g. proteins and bacteria) cannot penetrate this flat zone.
    Type: Grant
    Filed: June 17, 2014
    Date of Patent: December 19, 2017
    Assignee: Massachusetts Institute of Technology
    Inventors: Rhokyun Kwak, Jongyoon Han
  • Publication number: 20170307488
    Abstract: In accordance with an embodiment of the invention, there is provided a method for: a) high-throughput, multiplexed, affinity-based separation of proteins—especially low abundance proteins—from complex biological mixtures such as serum; and b) high-throughput, multiplexed, affinity-based separation of cells—especially rare cells—from complex biological mixtures such as blood or blood fractions. The separation of proteins or cells is achieved based on differential binding to affinity-capture beads of different sizes and then sorting the protein-bound or cell-bound beads using the concept of centrifugal-induced Dean migration in a spiral microfluidic device. This method enables continuous-flow, high throughput affinity-separation of milligram-scale protein samples or millions of cells in minutes after binding.
    Type: Application
    Filed: October 23, 2015
    Publication date: October 26, 2017
    Inventors: Aniruddh Sarkar, Han Wei Hou, Jongyoon Han, Galit Alter