Abstract: A heat and acid resistant probiotics microsphere having a size from 20 to 250 ?m that can readily be incorporated into food or beverages that subsequently undergo thermal treatment. The synbiotic core includes a seed layer formed from at least one polysaccharide. A probiotic microorganism is coated on the seed layer. An acid-resistant shell layer is positioned over the synbiotic core, the acid-resistant shell layer comprising one or more pH-responsive polymers. A heat-resistant bilayer shell is positioned over the acid-resistant shell layer, the heat-resistant bilayer shell including an inner shell layer and an outer shell layer, wherein the inner shell layer includes a heat-resistant liposome layer and the outer layer includes a heat-resistant disaccharide or polysaccharide.

Abstract: An inedible and dry dormant state encapsulated probiotic core-shell particle for external, non-mucosal skin application is provided. From inside out, the core-shell particle has a structure of a carrier particle core serving as a nutrient source for probiotics, a first layer including a dormant probiotic species for affecting epidermal biome and at least one prebiotic as a food source for the probiotic, a polymer layer positioned over the first layer, and a dissolvable protective layer for protecting the probiotic core-shell particle from oxidation, heat and humidity. By co-applying with a releasing medium, the dissolvable protective layer and the polymer layer are dissolved to expose the dormant probiotic containing layer, the first layer. Further, the releasing medium also is able to activate and reconstitute the dormant probiotic to a live probiotic on the applied non-mucosal epidermal surface.

Abstract: The present disclosure relates to salvianolic acid-gelatin conjugate load retentive hydrogel nanoparticles useful for oral delivery of salvianolic acid, pharmaceutical compositions comprising the same, and methods of use and preperation thereof.