Abstract: A method including one or more steps of a procedure for preparing a microscopic sample for a high-pressure freezing process is provided, wherein the sample is provided using an arrangement comprising a middle plate of a high-pressure freezing cartridge and an incubation chamber, wherein the middle plate is attached to a lower surface of a bottom of the incubation chamber by an adhesive and detachable from the incubation chamber by effecting a relative movement between the middle plate and the incubation chamber, wherein the sample is provided on an enclosing element which is fitted into an opening of the middle plate, and wherein, when the sample is to be subjected to the high-pressure freezing process, the relative movement between the middle plate and the incubation chamber is effected, thereby detaching the middle plate with the sample from the incubation chamber. Means to implement the method are also part of the present disclosure.

Abstract: A method including one or more steps of a procedure for preparing a microscopic sample (1) for a high-pressure freezing process is provided, wherein the sample (1) is provided using an arrangement comprising a middle plate (33) of a high-pressure freezing cartridge (30) and an incubation chamber (100), wherein the middle plate (33) is attached to a lower surface of a bottom (120) of the incubation chamber (100) by an adhesive and detachable from the incubation chamber (100) by effecting a relative movement between the middle plate (33) and the incubation chamber (100), wherein the sample (1) is provided on an enclosing element (37) which is fitted into an opening (36) of the middle plate (33), and wherein, when the sample (1) is to be subjected to the high-pressure freezing process, the relative movement between the middle plate (33) and the incubation chamber (100) is effected, thereby detaching the middle plate (33) with the sample (1) from the incubation chamber (100).

Abstract: A system for irradiating biological material includes a platform for holding the biological material and a radiation source for directing X-ray radiation to the biological material. The platform includes platform elements each including a chamber for containing the biological material. The platform includes a frame structure for mechanically supporting the platform elements and for mechanically supporting a gas-supply system for supplying gas to the platform elements. The gas-supply system can be for example a gas cartridge. The frame structure includes gas channels for receiving the gas from the gas-supply system and for conducting the gas to the platform elements so as to provide a desired gas composition in the chambers during irradiation of the biological material.

Abstract: A cell culturing platform for modeling myelination in vitro comprises first cell chambers (102a, 102b) for containing somas of neuronal cells, a process chamber (103) connected to the first cell chambers with first channels, and a second cell chamber (105) for containing somas of the myelinating cells and connected with second channels to the process chamber. The first channels allow processes of the neuronal cells to enter the process chamber and inhibit the somas of the neuronal cells from moving from the first cell chambers to the process chamber. The second channels allow processes of the myelinating cells to enter the process chamber and inhibit the somas of the myelinating cells from moving from the second cell chamber to the process chamber. Hence, the process chamber, where the myelination takes place, is substantially free from the cell somas and thus detection of the myelination is facilitated.