Abstract: The present disclosure concerns the development of personalised periodontitis score to assess the individual level of microbial imbalances in periodontal pockets and/or saliva of a specific individual at a defined stage of treatment, relative to a predicted expected score for the average patient with similar systemic and local medical characteristics and demographic profile. The microbial profile obtained from genetic/genomic information is combined with clinical and demographical information in a mixed-effect model to obtain a predicted value and a standard error for a given set of clinical parameters. The comparison between this predicted average score and the individual patient personalised periodontitis score informs about the relative periodontal disease activity in this site and/or patient and thus allows for the stratification of the local site and/or the patient into risk categories and for the recommendation of targeted patient-specific treatment modalities.

Abstract: The present invention relates to a DNA profiling assay comprising the following steps, providing a sample to be analyzed, providing reagents, enzyme and primeroligonucleotides which are necessary for simultaneous polymerase chain reaction amplification of at least 20 loci, amplifying the loci, detecting the amplification products, wherein the amplification products and the loci to be amplified are characterized by the following features, each locus to be amplified is characterized by at least one deletion-insertion polymorphism known to be present in the population, wherein the two alleles from each locus differ in size by more than 2 nucleotides and less than 100 nucleotides, a first set of at least two amplification products ranging in size from about 20 nucleotides to about 300 nucleotides stemming from at least two different loci carries a first label, a second set of at least two amplification products ranging in size from about 20 nucleotides to about 300 nucleotides stemming from at least two different

Abstract: The invention relates to a kit comprising, (a) a first group of containers, comprising two or more containers with human genomic DNA, wherein the human genomic DNA in each of the two or more containers is of different human origin and the amount of DNA in the container is defined, (b) a second group of containers, comprising two or more containers with human genomic DNA, wherein the concentration of the DNA varies between the containers and the concentration of the DNA in the container is defined and (c) a third group of containers, comprising two or more containers with human genomic DNA, wherein the human genomic DNA in each container is a mixture of genomic DNA and is from at least two different individuals. The invention also relates to an evaluation tool and methods of use of the kit.

Abstract: The present invention relates to a DNA profiling assay comprising the following steps, providing a sample to be analyzed, providing reagents, enzyme and primer oligonucleotides which are necessary for simultaneous polymerase chain reaction amplification of at least 20 loci, amplifying the loci, detecting the amplification products, wherein the amplification products and the loci to be amplified are characterized by the following features, each locus to be amplified is characterized by at least one deletion-insertion polymorphism known to be present in the population, wherein the two alleles from each locus differ in size by more than 2 nucleotides and less than 100 nucleotides, a first set of at least two amplification products ranging in size from about 20 nucleotides to about 300 nucleotides stemming from at least two different loci carries a first label, a second set of at least two amplification products ranging in size from about 20 nucleotides to about 300 nucleotides stemming from at least two differen

Abstract: The invention relates to a method for enriching and tracking pathologic modified prions-proteins (PrPsc) of living organisms.