Patents by Inventor Jorg Windisch

Jorg Windisch has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Expression vectors, transformed host cells and fermentation process for the production of recombinant polypeptides
    Patent number: 7785830
    Abstract: The present invention relates to an expression vector, comprising a polynucleotide encoding a fusion protein comprising the signal sequence of the gac gene of Pseudomonas diminuta and a polypeptide of interest, a prokaryotic host cell transformed with such an expression vector and a process for production of a polypeptide of interest using said host cell and said expression vector.
    Type: Grant
    Filed: August 12, 2004
    Date of Patent: August 31, 2010
    Assignee: Sandoz AG
    Inventors: Jörg Windisch, Kurt Schoergendorfer, Norbert Palma, Franz Knauseder, Hans Boehling
  • Production of proteins by autoproteolytic cleavage
    Patent number: 7378512
    Abstract: The present invention relates to a process for the production of a heterologous polypeptide with homogeneous N-terminus in a bacterial host cell, wherein the heterologous polypeptide is autoproteolytically cleaved from an expressed fusion protein which comprises a polypeptide with the autoproteolytic activity of an autoprotease Npro of a pestivirus and the heterologous polypeptide by the Npro autoproteolytic activity.
    Type: Grant
    Filed: January 23, 2004
    Date of Patent: May 27, 2008
    Assignee: Biochemie Gesellschaft m.b.H
    Inventors: Tillmann Rumenapf, Heinz J Thiel, Jorg Windisch, Franz Knauseder
  • Expression vectors, transformed host cells and fermentation process for the production of recombinant polypeptides
    Publication number: 20060228783
    Abstract: The present invention relates to an expression vector, comprising a polynucleotide encoding a fusion protein comprising the signal sequence of the gac gene of Pseudomonas diminuta and a polypeptide of interest, a prokaryotic host cell transformed with such an expression vector and a process for production of a polypeptide of interest using said host cell and said expression vector.
    Type: Application
    Filed: August 12, 2004
    Publication date: October 12, 2006
    Inventors: Jorg Windisch, Kurt Schoergendorfer, Norbert Palma, Franz Knauseder, Hans Boehling
  • Production of heterologous proteins using an Npro autoprotease of a pestivirus and inclusion bodies
    Patent number: 6936455
    Abstract: The present invention relates to a process for the recombinant production of a desired heterologous polypeptide with a clearly defined homogenous N-terminus in a bacteria host cell. A fusion comprising a peptide with the autoproteolytic activity of an autoprotease Npro of a pestivirus and the heterologous polypeptide is initially expressed in the form of cytoplasmic inclusion bodies in the host cell, the inclusion bodies are isolated and subsequently treated so that the desired heterologous polypeptide is cleaved autoproteolytically by the Npro activity of the fusion protein.
    Type: Grant
    Filed: August 7, 2000
    Date of Patent: August 30, 2005
    Assignee: Sandoz AG
    Inventors: Günter Stempfer, Jörg Windisch, Franz Knauseder
  • Production of proteins
    Publication number: 20050186564
    Abstract: The present invention relates to a process for the recombinant production of a desired heterologous polypeptide with a clearly defined homogenous N-terminus in a bacterial host cell. A fusion protein comprising a peptide with the autoproteolytic activity of an autoprotease Npro of a pestivirus and the heterologous polypeptide is initially expressed in the form of cytoplasmic inclusion bodies in the host cell, the inclusion bodies are isolated and subsequently treated so that the desired heterologous polypeptide is cleaved autoproteolytically by the Npro activity of the fusion protein.
    Type: Application
    Filed: April 5, 2005
    Publication date: August 25, 2005
    Inventors: Gunter Stempfer, Jorg Windisch, Franz Knauseder
  • Method for producing a recombinant polypeptide
    Publication number: 20050084969
    Abstract: The invention relates to a method for producing a transformed eukaryotic host cell which expresses a recombinant polypeptide of interest which method comprises introducing into a eukaryotic host cell: (a) a first polynucleotide vector which comprises (i) a first nucleotide sequence which encodes a recombinant polypeptide of interest, and (ii) a second nucleotide sequence encoding a selectable marker, which second nucleotide sequence is amplified when the host cell is contacted with a selection agent; and (b) a second polynucleotide vector having essentially the same nucleotide sequence as the first polynucleotide vector except that the second nucleotide sequence is replaced with a third nucleotide sequence which encodes a different selectable marker; the first polynucleotide vector and second polynucleotide vector being stably integrated into the genome of the host cell.
    Type: Application
    Filed: November 26, 2002
    Publication date: April 21, 2005
    Inventors: Kurt Schorgendorfer, Jorg Windisch, Renate Kunert, Florian Unterluggauer
  • Production of proteins by autoproteolytic cleavage
    Publication number: 20040215008
    Abstract: The present invention relates to a process for the production of a heterologous polypeptide with homogeneous N-terminus in a bacterial host cell, wherein the heterologous polypeptide is autoproteolytically cleaved from an expressed fusion protein which comprises a polypeptide with the autoproteolytic activity of an autoprotease Npro of a pestivirus and the heterologous polypeptide by the Npro autoproteolytic activity.
    Type: Application
    Filed: January 23, 2004
    Publication date: October 28, 2004
    Inventors: Tillmann Rumenapf, Heinz Jurgen Thiel, Jorg Windisch, Franz Knauseder