Abstract: A method for ensuring that gene products used in cell therapy do not carry a risk of reduced efficacy or toxicity due to production of unintended variants. The method includes performing an in-silico analysis on the gene construct to identify and alter sequences likely to cause variants. Also, the method includes performing an in-vivo analysis consisting of RNA-sequence of construct based products. Variant detection may then be performed based on gapped reads from the RNA-sequence to determine variant expression levels, variant significance. The method may include repeating the in-silico analysis if identified variants are unacceptable.

Abstract: The debris removal container (10) includes interiorly projecting strengthening ribs, such as ribs (25-29) that reduce the frictional contact between adjacent containers when one container is being nestably stacked in a lower container. The removable fork receptacle feet (14 and 15) may be replaced when damaged. The nested containers are placed in an upright, nested, stacked relationship for transport by a truck (44) and the containers are formed of light weight material so that a rotary forklift can lift and manage a nested stack, by inverting or resting the containers on their sides on a horizontal surface and picking the lowermost container from the nested stack for distribution at a construction site.