Abstract: In a method for enhancing accuracy and efficiency in automated visual inspection of vessels, a vessel containing a sample is oriented such that a line scan camera has a profile view of an edge of a stopper of the vessel. A plurality of images of the edge of the stopper is captured by the first line scan camera while spinning the vessel, where each image of the plurality of images corresponds to a different rotational position of the vessel. A two-dimensional image of the edge of the stopper is generated based on at least the plurality of images, and pixels of the two-dimensional image are processed, by one or more processors executing an inference model that includes a trained neural network, to generate output data indicative of a likelihood that the sample is defective.