Abstract: In certain embodiments, the invention relates to systems and methods for in vivo tomographic imaging of fluorescent probes and/or bioluminescent reporters, wherein a fluorescent probe and a bioluminescent reporter are spatially co-localized (e.g., located at distances equivalent to or smaller than the scattering mean free path of light) in a diffusive medium (e.g., biological tissue). Measurements obtained from bioluminescent and fluorescent modalities are combined per methods described herein.

Abstract: The invention describes a device (1) for characterizing the rough profile of a tissue sample comprising: a laser source (2) that illuminates the surface (100) of the tissue; a photodetector (3) that receives the light backscattered by the surface (100) of the tissue; and further a displacement means (4) configured to alternate between a first position wherein a rotating ground glass (5) is disposed within the path of the laser beam towards the surface (100), a second position wherein a rotating half wave blade (6) is disposed within the path of the laser beam towards the surface (100); and a third position wherein within the path of the laser beam towards the surface (100) neither the ground glass plate (5) nor the half wave blade (6) are arranged, or the half wave blade (6) is arranged in a fixed non-rotating position.

Abstract: An objective lens switching device includes a prismatic cuvette perpendicular to a plane parallel to a direction of detection and a lighting direction, which are mutually perpendicular. The cross-section of the prism is a polygon with more than four sides with pairs of mutually perpendicular faces. At least several pairs of faces include one face designed to receive a planar light beam in the lighting direction and one face with a coupled lens or objective lens to detect fluorescent light in the direction of detection. The cuvette can rotate about an axis perpendicular to said plane, allowing a specific objective lens to be oriented in the direction of detection.

Abstract: In certain embodiments, the invention relates to systems and methods for in vivo tomographic imaging of fluorescent probes and/or bioluminescent reporters, wherein a fluorescent probe and a bioluminescent reporter are spatially co-localized (e.g., located at distances equivalent to or smaller than the scattering mean free path of light) in a diffusive medium (e.g., biological tissue). Measurements obtained from bioluminescent and fluorescent modalities are combined per methods described herein.

Abstract: In certain embodiments, the invention relates to systems and methods for in vivo tomographic imaging of fluorescent probes and/or bioluminescent reporters, wherein a fluorescent probe and a bioluminescent reporter are spatially co-localized (e.g., located at distances equivalent to or smaller than the scattering mean free path of light) in a diffusive medium (e.g., biological tissue). Measurements obtained from bioluminescent and fluorescent modalities are combined per methods described herein.

Abstract: The invention describes an objective lens switching device (1) comprising a prismatic cuvette (2) perpendicular to a plane parallel to a direction of detection (DD) and a lighting direction (DI) which are mutually perpendicular. The cross-section of the prism is a polygon with more than four sides with pairs of mutually perpendicular faces (2a, 2a?; 2b, 2b?; 2c, 2c?; 2d, 2d). Furthermore, at least several pairs of faces (2a, 2a?; 2b, 2b?; 2c, 2e; 2d, 2d) comprise one face (2a?, 2b?, 2e, 2d?) designed to receive a planar light beam (6) in the lighting direction (DI) and one face (2a, 2b, 2c, 2c) with a coupled lens or objective lens (9a, 9b, 9c, 9d) to detect fluorescent light (8) in the direction of detection (DD). The cuvette (2) can rotate about an axis perpendicular to said plane, allowing a specific objective lens (9a, 9b, 9c, 9d) to be oriented in the direction of detection (DD).

Abstract: The invention relates to a microscope and a method for producing 3D images of various transparent or semi-transparent samples, fundamentally comprising: causing a relative movement according to the detection direction between the sheet of light and the sample while maintaining a constant angle of acquisition; producing, for said angle of acquisition, a single 2D projection image formed by a representative parameter for each pixel; modifying the angle of acquisition by means of a relative rotation between the sheet of light and the sample, combined with a relative vertical translation between the sheet of light and the sample, and repeating the previous steps; and generating a 3D image of each of the samples from the set of 2D projection images that are produced.

Abstract: The invention relates to systems and methods for tomographic imaging of a subject comprising diffuse media by converting measurements of electromagnetic radiation, e.g., fluorescent light, obtained in free space exterior to the subject into data that would be measured if the subject were surrounded by an infinite and homogeneous diffusive medium, e.g., a medium with optical properties equal to the average optical properties of the subject. After applying a transformation to convert measurements to virtually-matched values, propagation of light is simulated from the index-matched surface to a set of virtual detectors exterior to the subject and arranged in a geometrically advantageous fashion, for example, in a planar array, thereby facilitating the use of fast reconstruction techniques.

Abstract: The invention relates to a microscope and a method for producing 3D images of various transparent or semi-transparent samples, fundamentally comprising: causing a relative movement according to the detection direction between the sheet of light and the sample while maintaining a constant angle of acquisition; producing, for said angle of acquisition, a single 2D projection image formed by a representative parameter for each pixel; modifying the angle of acquisition by means of a relative rotation between the sheet of light and the sample, combined with a relative vertical translation between the sheet of light and the sample, and repeating the previous steps; and generating a 3D image of each of the samples from the set of 2D projection images that are produced.

Abstract: A method for tomographic imaging of diffuse medium includes directing waves into a diffusive medium, solving a surface-bounded inversion problem by forward field calculations through decomposition of contributions from the multiple reflections from an arbitrary surface within the diffusive medium or outside the diffusive medium into a sum of different orders of reflection up to an arbitrary order, and using contact or non-contact measurements of waves outside said diffusive medium to generate a tomographic image.

Abstract: A method for tomographic imaging of diffuse medium includes directing waves into a diffusive medium, solving a surface-bounded inversion problem by forward field calculations through decomposition of contributions from the multiple reflections from an arbitrary surface within the diffusive medium or outside the diffusive medium into a sum of different orders of reflection up to an arbitrary order, and using contact or non-contact measurements of waves outside said diffusive medium to generate a tomographic image.

Abstract: A method for tomographic imaging of diffuse medium includes directing waves into a diffusive medium, solving a surface-bounded inversion problem by forward field calculations through decomposition of contributions from the multiple reflections from an arbitrary surface within the diffusive medium or outside the diffusive medium into a sum of different orders of reflection up to an arbitrary order, and using contact or non-contact measurements of waves outside said diffusive medium to generate a tomographic image.

Abstract: In certain embodiments, the invention relates to systems and methods for in vivo tomographic imaging of fluorescent probes and/or bioluminescent reporters, wherein a fluorescent probe and a bioluminescent reporter are spatially co-localized (e.g., located at distances equivalent to or smaller than the scattering mean free path of light) in a diffusive medium (e.g., biological tissue). Measurements obtained from bioluminescent and fluorescent modalities are combined per methods described herein.

Abstract: The invention relates to systems and methods for tomographic imaging of a subject comprising diffuse media by converting measurements of electromagnetic radiation, e.g., fluorescent light, obtained in free space exterior to the subject into data that would be measured if the subject were surrounded by an infinite and homogeneous diffusive medium, e.g., a medium with optical properties equal to the average optical properties of the subject. After applying a transformation to convert measurements to virtually-matched values, propagation of light is simulated from the index-matched surface to a set of virtual detectors exterior to the subject and arranged in a geometrically advantageous fashion, for example, in a planar array, thereby facilitating the use of fast reconstruction techniques.

Abstract: The invention relates to systems and methods for tomographic imaging in diffuse media employing a fast reconstruction technique. A hybrid Fourier approach is presented that enables the fast tomographic reconstruction of large datasets. In certain embodiments, the invention features methods of in vivo fluorescence molecular tomographic (FMT) reconstruction of signals, reporters and/or agents (i.e., contrast agents or probes) in a diffusive medium (e.g., a mammalian subject). The method preserves the three-dimensional fluorophore distribution and quantitative nature of the FMT approach while substantially accelerating its computation speed, allowing FMT imaging of larger anatomies.

Abstract: A method for tomographic imaging of diffuse medium includes directing waves into a diffusive medium, solving a surface-bounded inversion problem by forward field calculations through decomposition of contributions from the multiple reflections from an arbitrary surface within the diffusive medium or outside the diffusive medium into a sum of different orders of reflection up to an arbitrary order, and using contact or non-contact measurements of waves outside said diffusive medium to generate a tomographic image.

Abstract: The invention relates to systems and methods for tomographic imaging of a subject comprising diffuse media by converting measurements of electromagnetic radiation, e.g., fluorescent light, obtained in free space exterior to the subject into data that would be measured if the subject were surrounded by an infinite and homogeneous diffusive medium, e.g., a medium with optical properties equal to the average optical properties of the subject. After applying a transformation to convert measurements to virtually-matched values, propagation of light is simulated from the index-matched surface to a set of virtual detectors exterior to the subject and arranged in a geometrically advantageous fashion, for example, in a planar array, thereby facilitating the use of fast reconstruction techniques.

Abstract: The invention relates to systems and methods for tomographic imaging in diffuse media employing a fast reconstruction technique. A hybrid Fourier approach is presented that enables the fast tomographic reconstruction of large datasets. In certain embodiments, the invention features methods of in vivo fluorescence molecular tomographic (FMT) reconstruction of signals, reporters and/or agents (i.e., contrast agents or probes) in a diffusive medium (e.g., a mammalian subject). The method preserves the three-dimensional fluorophore distribution and quantitative nature of the FMT approach while substantially accelerating its computation speed, allowing FMT imaging of larger anatomies.